We narrowed to 362 results for: tac

1. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   ...familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas ...

2. Developing Lab Management Software for Biology

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   Blog Post

   ... valid technical reasons to choose a non-Python stack, but if you're working with scientists it's unlikely...

3. Gendered Innovations: Why Does Sex of the Cell Matter?

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   Blog Post

   ... gender analysis for innovation and discovery. Tackling sex and gender in research Governments, universities...

4. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   Blog Post

   ...understand complex biological systems. He can be contacted at [email protected].   References 1. Cheng, Allen A...

5. Overcoming the Challenges of Lentiviral Production

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   Blog Post

   ...supernatant is collected, filtered, and titered. A major obstacle in obtaining usable lentivirus is producing a ...

6. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   ...cell. Their Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of...

7. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   ...a gene while leaving other features of the gene intact. Here, we show an example gene targeted for tagging...

8. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   ...expression manipulation. They are injected using a stereotactic frame into a defined brain region that is suspected...

9. Corralling and Cornering the Goldilocks Antibody

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   Blog Post

   ... antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman is a research assistant in...

10. A Look at Addgene's QC Process

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    Blog Post

    ...If the QC team identifies a QC issue, they will contact the depositing lab to review the discrepancies....

11. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post

    ...effects since promoter and enhancer sequences remain intact (Engreitz et al. 2019). The interactions between...

12. Viral Vectors 101: Calcium Sensors

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    Blog Post

    ...indicators, these DNA constructs can be delivered to intact tissues and organs noninvasively and target a defined...

13. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    ...higher risk cargo for a researcher to come into contact with than GFP. Similarly, if the viral vector carries...

14. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ...pattern and an auxin inducible degron (AID) sequence attached to your protein of interest. TIR1 interacts with...

15. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...and having fun with technology, so feel free to contact your local space and get involved! Derek Jacoby...

16. Fluorescent Protein Guide: Biosensors

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    Collection

    ... GFP-Based cGMP Sensor for Real Time Imaging in Intact Caenorhabditis elegans. Genetics. 2019 Jul 22. ...

17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ...available near the locus you’d like to modify. To tackle the PAM problem, researchers have engineered alternative...

18. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    ...target site, Cas9’s DNA binding capability remains intact while its nucleolytic activity is eliminated (Fig...

19. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    ...or questions from your poster viewers and trade contact information if your conversation is particularly...

20. Degrading DNA with Cascade-Cas3

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    Blog Post

    ...portion can be bypassed by leaving out Cas3 — a tactic some CRISPR transposase systems utilize with type...