...genetically encoded way. SpyTag is nice and small, just 13 amino acids, but SpyCatcher is a protein. You...SpyCatcher onto things, but even better if one could just join one peptide tag to another peptide tag. Addgene... there any major hurdles along the way? Howarth: Just the usual blood, sweat and tears. (Thanks to our...of CTCs to cope with the variation in cancer, not just the CTCs with high marker expression level. Labs...

...containing substantial autofluorescence background.” Just last month in Scientific Reports, Verkhusha’s team...fluorescent proteins, iRFP670 and iRFP720, as photoacoustic contrast agents for two-color imaging in animals...millimeters – not single cells (yet) but small clusters of cells. Looking ahead What’s next, you might...: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent...

...other summer programs. Amy took into consideration just how busy I was and ensured that my decision deadline...program, teaching me the basics of physics and sustainable design while allowing me to do my internship ...opportunities and support, puts into perspective just how much one’s environment changes them. Addgene...-Villagran is a rising senior in high school who just completed their summer internship at Addgene. They...

...repository onto one handy Microbiology Resource page and, just a few weeks ago, we attended the American Society...applying temporary tattoos on the spot with our trusty spray bottles! We also raffled off one of our highly...see below) on my way back from getting a badge illustrated with my favorite microorganism (Rhodobacter sphaeroides... deposit and distribution service! Did you see a must-have plasmid during ASM? Let us know what’s missing...

... email to deliver corrective feedback. A manager must be able to respond to the employee’s level of discomfort...do differently? Beware of indirect language You must be honest about performance problems. While giving...indirect language from Steven Pinker. As a manager you must be able to state your observations truthfully and...helpful feedback gets easier with practice so you must build it into your routines. This is the third ...

...finding a job. Many people in science will work in industry or take on collaborative roles in academia, and...know how to talk about science to everyone – not just to others working in your own field. Alternative...It’s useful for non-scientists to understand not just the details of your science but also something about...communicating your research by guest blogging here, just leave a comment if you're interested and our team...

...applications can be exhausting. A quick search for "CRISPR", short-hand for Clustered Regularly Interspaced...to ask questions and get answers on the fly. But just like the growing PubMed article list, the Genome...forum's most popular questions and answers. Here are just 4 of the CRISPR-Cas9 FAQs. If you still have more...

...begin distributing a material, a deposit agreement must be put in place. And subsequently, every time that...requested, a material transfer agreement (usually) must be executed. The Golden Stopwatch Award highlights... Addgene Master Agreements. By default, your TTO must sign an agreement each time a material is being ...Deposit Agreements, your TTO signs each agreement just once, covering nearly all future requests/deposits...

...months past a worldwide shift to work-from-home and just now realizing that we can’t bide our time and wait...need to be more senior or more “important”, they just need to know you well enough to ask good questions...be as successful as in person groups. Maybe it’s just that it’s easier for everyone to attend all the ...with the process. Bonus points for being able to just look at a paragraph and do a character count in ...

...the conference, I think I may have finally grasped just how complicated DNA repair pathways are. I fully...There is so much more to working with CRISPR than just designing a repair template that contains your desired...Maybe you’d like to avoid editing the genome and just stick to RNA? Cas13 may be able to help you do that...Cpf1. This tool supports over 250 genomes, and they just released a brand new CRISPOR manual to kick off ...

...discernible paths. The first leads into the world of industry. Along this road, science communication graduates...respond to issues of unsettled science. Understanding just how the public utilizes insights gleaned from scientific...stroll from my office to the tearoom would often illustrate the breadth of knowledge and passion on display...day, it has never been more crucial to understand just how we receive, process and respond to that information...

...contaminating AAV DNA. In addition, we wrote an open-source custom Python script that uses the VGS data to determine...we think of the AAV vector genomes as containing just the expression cassette that we want, it also includes...data in minutes and the scripts  can be easily customized to detect other sequences of interest.   While...Ayuso E, Vandenberghe LH (2017) AAV-ID: A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation...

...ordering viral vectors. Virus production can be a frustrating process that requires a large investment of time...have experience with it. We’re reducing this frustration and getting researchers to the experiments they... lentiviruses are used in experiments other than just those involving CRISPR and so we also have lentivirus...viral transductions, and overviews for beginners just starting work with virus. You can find all of our...

...Reddit AMA Add a little formality to mentoring Just getting together and chatting with another person...it to clarify and discuss values. The activity is just the start – it is the discussion after that will...mentoring journal will help a Mentee track progress, adjust expectations, and record milestones in reaching...to complete action items and make progress. To illustrate this point, consider that I try to exercise every...

...student hasn’t been asked the question: “Academia or industry?” Once academia was the clear answer with a well-defined...uses it to match qualified scientists to jobs in industry, academia, and government. This great resource...pathways, education and mastering new skill sets must occur by doing work outside of the lab. Extracurricular... more information Follow people in academia, industry, and government on social media (Twitter, Facebook...

...scientist has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA...process can be a bit confusing, and it’s certainly frustrating to find out your plasmid hasn’t shipped because...have the option of distributing under Addgene’s Industry MTA (IMTA), which allows the plasmids to be distributed...distributed for research use only to industry-based scientists. Some organizations choose to share their...

...image below shows:    This system ablates tumors just as effectively as standard CAR-T cells, but it reduces... is elevated at stable levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one...antibodies. But sometimes, one antibody per target just isn’t enough and we recently released Anti-GFP [...monoclonal. Another reason is that some antibodies may just not perform as well in one application as another...

...reads, this analysis must be done in an automated manner. Addgene commonly uses a custom script derived from...And since the library contains many plasmids, care must be taken to ensure that all of these plasmids are...individual plasmid in the library. Bacterial growth must also be optimized to maintain library diversity....

...Follow your contact’s company on LinkedIn (or via industry newsletters such as FiercePharma), or sign up ..., suggest a coffee chat in the near future. Even just one more meeting (in person or virtually) will make...introduction that helped someone get a job interview. Even just a thank you email would have been enough, but I’...and interests. Offer help when you can, instead of just asking for favors.  Don’t focus on networking with...

...involvement, research, and consensus can happen. We just have to look to the United Kingdom’s Nuffield Council...things happened nearly simultaneously: The Wellcome Trust funded a center for mitochondrial research to investigate...decide on the future of genome editing technology. We must stop punting decisions on scientific, ethical, regulatory...utilizes her firsthand experience in the software industry to inform her work on emerging issues in novel...