We narrowed to 532 results for: abo.3

...Teacher, Vol. 79 No. 3, March 2017; (pp. 208-223). Link: http://abt.ucpress.edu/content/79/3/208 2. Fire, S....Nature, 391 (1998), pp. 806-811. PubMed PMID: 9486653. 3. Newmark, Phillip A., et al. "Ingestion of bacterially...experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology... Biology with BioBuilder 3D Models for learning about CRISPR Resources at Addgene.org Find plasmids ...

...implement RSMs in E. coli with two plasmids (Figure 3 below): Input Plasmid: The input plasmid (high copy...and promoters with buffer sequences on their 5’ and 3’ ends (for insulation). A list of promoters and terminators...hammerhead ribozyme parts are described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register...PMID: 25417166. PubMed Central PMCID: PMC4265554. 3. Nielsen, Alec AK, et al. "Genetic circuit design ...certain cellular behaviors, but little is known about the order or timing of these factors. Recombinase-based... three methods of state interrogation mentioned above (Sanger sequencing, qPCR, and fluorescent reporter-based...Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from Grad Student to SynBio...

...covered (to avoid drying out) while 3 ml of melted agarose are mixed with 3 ml of 2X DMEM in a 50 ml sterile...easily achieved by splitting transfected cells into 3 plates at different densities for selection). A 2%...specific genes. Many years ago, people in the laboratory became frustrated with using glass cloning rings...the hood. A 48-well plate is also prepared with about 200 ul of culture medium containing serum in each...incubator, additional growth medium is added to about halfway up each well. Tips for using the agarose...with the firmer 2% agarose gel solution discussed above for picking. Many thanks to our guest blogger Iris...

...Examples of these approaches are given in Tables 3-7. 3. Making use of AAV’s selectivity for neurons: targeting...Learn more about retrograde AAVs Resources on Addgene.org Read our AAV guide Learn more about our viral...

...transgene contains a splice site acceptor followed by the 3’-end of the transgene. When a cell is transduced by...80% of muscle cells expressing mini-dystrophin.  3. Hybrid  Overview: The hybrid approach combines the...region of homology, a splice acceptor sequence, the 3’ half of the transgene. The full length gene is reconstituted...upstream of the 5’ splice donor and downstream of the 3’ splice acceptor of the transgene to help prevent ...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

...efficiency by approximately 15-fold (Chen et al., 2006). 3. Transduction efficiency depends on viral titer Both...approximately 20–50% of the packaging cells after 1–3 days of selection. A lower percentage of surviving...." Cell 113.2 (2003): 141. PubMed PMID: 12705861. 3. Chen, Sifeng, et al. "Freeze-thaw increases adeno-associated...grown at 30 °C instead of 37 °C. If you’re unsure about the integrity of your virus, perform a diagnostic...Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus New to Virus? Check ...Check out Our Primer for Beginners! Learn All about Lentiviral Vectors Additional Resources on the Addgene.org...

...with us about adding new scientists to their labs. Read All the Posts in this Blog Series Our 3 PIs answer...'ve heard of labs where new hires spend the first 3 months reading and writing a proposal before even ...research question that people (especially you!) care about. Have you critically evaluated your plan? Have... to complete and has multiple benchmarks. Think about what your personal goals are and set milestones .... Take the time to generate new ideas and think about them critically before diving in. Research projects...

... Core hours consist of the time between 10 AM and 3 PM, when most meetings are scheduled to take place...For example, if I needed to go to an appointment at 3 PM, I could make up the hours I had missed at another...intimidating to me, as I had a hard time thinking about how I could fill this time. I was relieved to find...attend to other commitments, without feeling guilty about missing work. As long as my work was finished on...

... microscopy. Nanobodies can be labeled with up to 3 dyes, yielding similar signal strength to a standard...nanobodies are useful for colocalization labeling of up to 3 targets (Pleiner et al figure 4D). Unlike large secondary...PMID: 26633879 PubMed Central PMCID: PMC4755751   3. Muyldermans, Serge. “Nanobodies: natural single-domain...Control Protein Activity Learn more about protein tags Read about E. coli strains for protein expression..., if not better, than antibodies for all of the above listed molecular techniques, but they can also be...protein purification methods. Read on to learn more about nanobodies and how their structure and function ...between a fluorescent tag and the target antigen to about 2 nm, which can lead to higher resolution for super-resolution...

...selection techniques to come up with new colors (Fig. 3), such as Radish and Carrot, and further means to ...imaging in situ”. Nature Methods 9, 787–790 (2012). 3. Filonov, Gregory S., et al. “In-gel imaging of RNA...RNA-Modifying Enzymes.” Cell Chem Biol. 2016 Mar 17;23(3):415-25. PubMed PMID: 26877022. PubMed Central PMCID...target metabolite creates an aptamer that will only bind to DFHBI in the presence of that metabolite (Fig...approach less sensitive in most applications. Learn About Fluorescent Biosensors Light-up aptamers (or, fluorescing...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein Activity with GFP Resources...