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  1. Immunology Research Plasmids and Resources

    Type
    Collection
    ...immunoglobulin heavy diversity 3-22 IGHD322 IGHD3-3 immunoglobulin heavy diversity 3-3 DXP4, IGHD33 IGHD3-9 immunoglobulin...VH IGHV3-30-3 immunoglobulin heavy variable 3-30-3 IGHV3-3, IGHV3303 IGHV3-30-5 immunoglobulin heavy variable...motif) ligand 3-like 2 G0S19-3, LD78gamma, SCYA3L2 CCL3L3 chemokine (C-C motif) ligand 3-like 3 464.2, D17S1718... variable 3-30-5 IGHV3-3, IGHV3305 IGHV3-33 immunoglobulin heavy variable 3-33 IGHV333, VH IGHV3-35 immunoglobulin...lambda variable 3-9 (gene/pseudogene) IGLV39, V2-6 IGLV4-3 immunoglobulin lambda variable 4-3 IGLV43, V5-1..., MRS DEFA3 defensin, alpha 3, neutrophil-specific DEF3, HNP-3, HNP3, HP-3 DEFA5 defensin, alpha 5, Paneth...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library 140633 Knockout Human Wei 3rd 3 58,630 Human...SAM v1 - 3 plasmid system 1000000057 (Zeocin) 1000000074 (Puromycin) Activation Human Zhang 3rd 3 70,290...70,290 SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid...177663, 177664, 177670 Inhibition Human Adamson 3rd ∼3 366 or 1,573 Activity-optimized genome-wide library...92380 (Set B) Activation Human Doench and Root 3rd 3–6 56,762 (Set A) 56,476 (Set B) Broad GPP activation...92384 (Set B) Activation Mouse Doench and Root 3rd 3–6 67,187 (Set A) 66,889 (Set B) Broad GPP inhibition...92386 (Set B) Inhibition Human Doench and Root 3rd 3–6 57,050 (Set A) 57,011 (Set B) Broad GPP inhibition...

  3. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ...RLD-1 nuclear receptor subfamily 1, group H, member 3 NR2E1 Plasmids NR2E1, RP3-429G5.1, TLL, TLX, XTLL ...group E, member 1 NR2F1 plasmids NR2F1, COUP-TFI, EAR-3, EAR3, ERBAL3, NR2F2, SVP44, TCFCOUP1, TFCOUP1 nuclear...NR3C1, GCCR, GCR, GR, GRL nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) NR3C2...MCR, MLR, MR, NR3C2VIT nuclear receptor subfamily 3, group C, member 2 NR4A1 Plasmids NR4A1, GFRP1, HMR...subfamily 4, group A, member 1 NR4A2 Plasmids NR4A2, HZF-3, NOT, NURR1, RNR1, TINUR nuclear receptor subfamily...nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling Atlas (NURSA...associated with numerous disease pathways. In collaboration with research labs from around the globe, Addgene...

  4. CRISPR Guide

    Type
    Collection
    ...EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus...SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3' NGCG SpCas9...jejuni (CJ) 3' NNNNRYAC Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW...characterized *In the table above, 3' and 5' indicate on which end of targeted sequence the PAM is located... DNA target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting sequence) will begin to...gRNA will continue to anneal to the target DNA in a 3′ to 5′ direction. The location of any potential mismatches...matters. Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...

  5. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...with four different phosphobinding domains (14-3-3β, 14-3-3σ, the WW2 domain of NEDD4, and the WW2 domain...111881 pNAS1b split mCherry 14-3-3σ Plasmid 111880 pNAS1b split mCherry 14-3-3β Pooled Library 111704 Mode...111705 Mode #2 Library (14-3-3β) Pooled Library 111706 Mode #2 Library (14-3-3σ) Pooled Library 111707 ...iSPI_pSer_Subpool#2 Pooled Library 188528 iSPI_pSer_Subpool#3 Pooled Library 188529 iSPI_pSer_Subpool#4 Pooled Library...52055 EcAR7 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid 112030 pNAS1b split mCherry NEDD4 ...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

  6. Ras Pathway

    Type
    Collection
    ... AKT3 v-akt murine thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35... factor receptor FLT3 Fms related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ...PDGFRB Platelet derived growth factor receptor PDPK1 3-phosphoinositide dependent protein kinase 1 PEBP1 ...known as PI3K; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I...cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763 . Do you have suggestions... Frederick National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...sequence in the 3' UTR David Bartel 12179 pIS1 Renilla Insertion of target sequence in the 3' UTR David Bartel...the middle of the Firefly luciferase gene.. TREAT (3( T hree)′- R NA E nd A ccumulation during T urnover... pIS2 Renilla Insertion of target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly...Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of...Atkins 167576 mpCHECK2 Firefly/Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly...Macho 136010 psi-CHECK3 Firefly/Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly...Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016 blog post...

  8. mTOR Pathway

    Type
    Collection
    ...homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as MAPKAP1...PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I...Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3):373-9. doi: 10.1016/j.cmet.2014.01.001. PubMed PMID... the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein...mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many...ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...Lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, and Blasticidin...tTA at its 5' end and an inducible promoter at its 3' end, which controls GFP expression tTA Off Verma ...contains insulator sequence; plasmid pEnt L1L3 rtTA-3 is a rtTA version tTA Off Hsiao 34856 fos-tTA Mouse...plus part of the first intron (-764/+918) and fos 3'UTR tTA Off Mayford 38056 pXCX.CMV.tTA Adenoviral;...Oesch F, Zabel B. Physiol Genomics . 2002 Dec 3;11(3):115-32. PubMed . Tetracycline derivatives: alternative... or shRNA; see article for detailed information about cloning and additional plasmids tetR-KRAB On Aebischer...

  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Mammals. Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression...Mammals. Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial...Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2...During Nutrient Transitions. ACS Sens. 2020 Mar 27;5(3):814-822. Bas Teusink ATP (extracellular) ecAT3.10...accumulation in bacteria. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose Green fluorescent MBP-based...Learning. Cell. 2020 Dec 12. pii: S0092-8674(20)31612-3. Lin Tian Serotonin Serotonin (5-HT) sensor psychLight...biosensor. Cell. 2021 Apr 8. pii: S0092-8674(21)00374-3. Lin Tian Serotonin Cilia-targeted serotonin sensor...

  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a 114477 Anti-TRPML3/Mucolipin-3 [N268/19R] TRPML3... victoria Mouse IgG2a 114493 Anti-NGL-3/LRRC4B [N51/6.2R] NGL-3/LRRC4B Rat Mouse IgG2a 114494 Anti-VGlut3...Rat Mouse IgG2a 114518 Anti-Neuroligin-3 [N110/29.4R] Neuroligin-3 Rat Mouse IgG2a 114519 Anti-RGS14 [N133...149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse IgG2a 149453 Anti-Kv4.2...Bassoon Rat Mouse IgG2a 177471 Anti-Synapsin-3 [L125/121R] Synapsin-3 Human Mouse IgG2a 177472 Anti-Pan-Synapsin...Mouse Mouse IgG2a 206608 Anti-Synapsin-3 [L125/76R] Synapsin-3 Human Mouse IgG2a 206610 Anti-Cav1.3 Ca2...Mouse Mouse IgG2b 206652 Anti-Neuroligin-3 [N110/29R-2b] Neuroligin-3 Rat Mouse IgG2b 206653 Anti-HCN4 [N114...

  12. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...pX330 vector using BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after the CACC sequence and ...nucleotide after the CACC sequence. Add a C at the 3’ end of the reverse complement oligo ( e.g., sgRNA-A...pX330 using a Golden Gate assembly cloning strategy 3 . Prepare a 50 μl reaction mix: 100 ng circular pX330...ml ampicillin and incubate O/N at 37 °C. Pick 2 - 3 colonies and inoculate into a mini-prep culture. Perform...50 μm filter into a FACS tube. FACS sort the top ~3% of GFP positive cells in order to enrich for cells...plate. Allow the bulk cells to incubate at 37 °C for 3 - 7 days and allow the clones to incubate at 37 °C...article from Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell lines using ...

  13. Lentiviral Prep Service

    Type
    Collection
    ...Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding...barcoding library. Version 3 of the Celltag library contains 5737 barcodes to combinatorially index cells for...are reported with each shipment. For more details about our viral vector packaging service, visit our Viral...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...) and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 alone: This plasmid contains...site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found that...Targeted gene activation using SAM There are two sets of 3 vectors each available for mammalian endogenous gene...sequence (21-22bp) and need to be followed on the 3' end by NGRRT or NGRRN PAM sequences. We have found...Jiang W, Marraffini LA, Zhang F. Science . 2013 Jan 3. DOI: 10.1126/science.1231143. PubMed . DNA targeting...Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids...

  15. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3):463-71. PMID: 19066593 Other citations include: Bogner...cell entry characteristics. Gene Ther . 2020 Apr;27(3-4):127-142. PMID: 31611639 AAV2(trpYF) When using ...Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. PMID: 26390090 Other citations include: Scalabrino...Information The plasmid below is available in each of the above serotypes and buffers. Please click on the plasmid...

  16. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... actual oligo you should order is: 5'-CACCGN...N-3' 3'-CN...NCAAA-5' You need to add the CACC and AAAC...region, will they also be targeted? The Cas9 cuts 3-4bp upstream of the PAM sequence. There can be some...)01015-5 As for target selection, we usually pick 3-6 guides around the region to find the most efficient... Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR technology. Genome..., February 2013 ), has assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ...development (embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding...spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor ...zebrafish strains and materials and information about zebrafish research. China Zebrafish Resource Center...America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A database of CRISPR...gene expression database that provides information about when genes are active in developing embryos. zfRegeneration...

  18. Viral Production

    Type
    Collection
    ... that titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard... appear darker than full vector particles. Figure 3: Electron micrograph of AAV vector preparation shows... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...

  19. Open Enzyme Collection

    Type
    Collection
    ...(exo-) DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-) 165512 pOpen-THEAQpolA Taq DNA Polymerase...MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) 165556 pOpen-MMLV_RT (lack H) Moloney Murine...engineering and directed evolution. More information about the collection can be obtained at the Open Bioeconomy...

  20. MAPK Plasmids

    Type
    Collection
    ...p38Beta MAPK12 ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3 MAPK13 RP1-179N16.4, MAPK 13, ... point for multiple biochemical signals. In collaboration with numerous research labs from around the ...
Showing: 1 - 20 of 40 results