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We narrowed to 819 results for: cell

Showing: 131 - 140 of 819 results

  1. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...Table 1 from the paper. 2. Manipulate your cell of interest: Cell-type specific expression of molecular tools...in their cell bodies. Optical stimulation in a different brain area can then reveal the cells bodies of...with AAV: 1. Getting your viral vector into your cell of interest: AAV tropism and route of administration...transduction, the viral capsid proteins bind to the target cell’s surface proteins and this interaction determines...efficiency of the AAV serotype used for a specific cell type. Find some transduction characteristics of ...by restricting expression to genetically defined cell types. Specific transgene expression can be also...different approaches can increase the specificity of cell targeting even further. For example, injecting AAVs...

  2. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...animation.)  Many types of immune cells, from mast cells to B cells, express Fc receptors that bind to...opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive when holes...(NK) cells expressing Fcγ receptors binds to IgG and release cytotoxins that kill the target cell. If ...response, by binding to Fc receptors on other immune cells. (psst! Need a quick review of the different immune...pathogen and physically prevent it from entering a cell, a non-Fc-dependent effector function known as neutralization...pathogen and mark it as a target for other immune cells, in a process known as opsonization. In antibody-dependent...shaping the immune response by driving particular cells or responses. And there are still antibody isotypes...

  3. Viral Production

    Type
    Collection
    ... preparations are added to cells in culture. Three to five days later, cell cultures are inspected for... a new vial of early passage cells. Approximately 2 weeks post-thaw, cell culture supernatant is tested...involves transducing cells with serial dilutions of the lentiviral vector, harvesting cells several days later...see maps section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre... (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445...Production Lentiviral preparations are produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging...packaging system developed by the Trono lab . Cell culture medium (OptiPro SFM) containing lentivirus is ...

  4. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...epithelial cells, liver, CNS (limited penetration), retina, hematopoietic cells, and immune cells. B) AAV11...the acquisition of leukemic driver mutations. Cell Stem Cell, 32(4), 564–580.e6. https://doi.org/10.1016...therapy and ex vivo cell engineering applications and is especially well-suited for lung cells via airway administration...Sanchez, P. S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing stem cell heterogeneity dictates clonal...plant genome editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging...filaments with mGold2. Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s, or ...many barcoding libraries are available for tracking cell lineage, different applications require careful ...

  5. Antibodies 101: Isotypes

    Type
    Blog Post
    ...the surface of basophils and mast cells, two types of white blood cells involved in allergic reactions. ... is important because it determines what immune cells and molecules are recruited by the antibody to help...each of these functions: Recruitment of immune cells that express receptors that recognize the Fc portion...special receptors that transport antibodies through cells and into different body compartments, such as into... during a primary immune response because all B cells begin by expressing IgM. IgM has a pentamer structure...coexpressed with IgM on the surface of most immature B cells, its function is unknown. It has a monomer structure...Isotype switching Isotype switching occurs when a B cell changes which heavy chain constant domain it pairs...

  6. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ... quantified for individual cells, and the number of 'normal' looking cells can be counted. This assay ...living cells. Strikingly, the OSER assay reveals that tagRFP does not act as a true monomer in cells (Constantini...homodimerize in cells. To understand how well fluorescent proteins approach this ideal situation, cellular assays...tolerated in some applications (e.g. labeling of cells or tracking promotor activity), but it is problematic...fluorescent proteins interact or oligomerize in cells? This question is largely unanswered. Ideally, fluorescent...proteins could properly form microtubule networks in cells. Other efforts tested whether FP fusions to connexin...present in EGFP. Constantini et al, (2012) developed a cell-based assay that does a better job of detecting ...

  7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...for neurons. Non-neuronal cells do this too. It's just that for non-neural cells, ion-based communication...between many cells (through gap junction proteins which connect the cytoplasm of two cells) can also affect...more depolarized (positive) the cell is, the more embryonic, stem cell-like, or cancerous it is (Levin...happens at a slightly slower scale.  All living cells with a membrane possess an electrical state. This...charge between the inside and the outside of the cell. This imbalance is maintained by ion channels and...proteins cx43 GJB1 GJB2 Bioelectric states can alter cell types and patterning There is mounting evidence ...more polarized (negative) the action potential of a cell, the more differentiated and quiescent it is, and...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...be applicable to studies of cell cycle progression, epigenetics and cellular reactions to external stimuli...Weissman JS, Qi LS, Huang B. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Pubmed. Find...LS, Weissman JS, Vale RD. Cell. 2014 Oct 23;159(3):635-46. doi: 10.1016/j.cell.2014.09.039. Epub 2014 Oct...labeled Cas9 to label multiple genomic loci in live cells. While other systems can be used to label loci, ...CRISPR/Cas9’s ease of use and ability to label live cells make this system truly advantageous. This new technique...nuclear organization varies across the life of a cell, or how organization may be altered in disease states...dynamics, and how they change in response to the cell cycle or other stimuli.   To create a colorful Cas9...

  9. Zebrafish Plasmid Collection

    Type
    Collection
    ... and track clones of cells over time. Examples of useful tools for studying cells that share clonal origin...Shendure Lab Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain - Alex...Mumm Lab. NTR 2.0/MTZ-based cell ablation system that allows sustained cell loss and ablation of ...Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies on genetic...below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation - Jeffrey...Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled...

  10. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...endocrine cells. It follows that the specific targeting of these voltage indicators needs to be cell type ...Indicators As previously mentioned, cellular voltage is measured at the cell membrane, and this voltage is ...electrical events! Voltage indicators are voltmeters for cells! In this blog we will review how these indicators...related to the electrical impulse of the cardiac cells. To study this phenomenon you will likely want to...the voltage change across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by... electrical charges propagated through multiple cells to do things like translate perceived signals to...Understanding neuronal pathways and the function of certain cells depends on the ability to monitor their signals ...
Showing: 131 - 140 of 819 results