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We narrowed to 728 results for: STO

Showing: 391 - 420 of 728 results

  1. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation... fused to a dead Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase...

  3. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...little simplified): Find the appropriate glycerol stock within our freezers Pick the sample into the appropriate...intentionally trivial, but they're here to help tell this story. In this first example to the right (# this will...

  5. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...drug resistance, Bhang says “the [drug] resistance story panned out quickly,” and was summarized in a recent...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

  6. A Look at Addgene's QC Process

    Type
    Blog Post
    ...Eventually, these errors could cause the plasmid to stop functioning properly or be something completely ...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains...

  7. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred... Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). A cryptic plasmid...

  8. Hot Plasmids - October 2020

    Type
    Blog Post
    ...2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies...color-changing fluorescent timer (FT) protein to the histone H2B that allows for  observation in live cells....

  12. CRISPR in the Clinic

    Type
    Blog Post
    ...Additional ongoing trials The CRISPR therapies don’t stop with the list above! There are many, many more in...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...

  13. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Resources References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity... https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ...

  14. Engaging with science and society at pgEd

    Type
    Blog Post
    ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical... talk about science in a language that can be understood by those who are not experts in particular scientific...

  15. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ... we'll provide an overview on how transcription stops, or termination. Read on to learn more! Download...basic processes for eukaryotic termination are understood and it has been noted that each eukaryotic RNA...

  16. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...genetics and anatomy, which are not yet fully understood. We attempt to deconstruct and understand this... into it! If you’re in the Victoria area, please stop by. And if not, I suspect you can find a makerspace...

  18. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies have shown that AAV11...purification, and titration. Can't wait? Browse our In-Stock AAV inventory or use our Packaged on Request service...

  19. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... cleaving of the target strand is not as well understood but likely occurs in similar regions. This combination...up to hundreds or even thousands of base pairs, stopping either at boundaries defined by anti-CRISPR proteins...

  20. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...particles you need are not available, or the cost of custom viruses are too high for your budget. In those ...round provides the added benefit of producing a stock P1 virus, so that if you ever need to remake the...

  23. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...late 1960s during research at hot springs in Yellowstone National Park.  In addition to Taq DNA polymerase... low during the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction...

  24. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...to tailor your presentation so that it can be understood by your audience. Use your poster as an aid: ... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often...

  28. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

  30. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid, and...
Showing: 391 - 420 of 728 results