We narrowed to 1,054 results for: STI

...potential for therapeutic drug discovery as it is estimated that 75% of disease causing genes in humans have...Stainer group determined that there are actually two distinct populations of β-cells that originate in different...other model organisms. Worms have been used to investigate an array of biological systems and processes ...PubMed PMID: 9486653. Hesselson, Daniel, et al. "Distinct populations of quiescent and proliferative pancreatic...19706417. PubMed Central PMCID: PMC2736433. Howe, Kerstin, et al. "The zebrafish reference genome sequence...

...epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive lasting transcriptional...cofactor fused to a histone tail to recruit and stimulate the endogenous methyltransferase. The strategy...H3K4me0 on D3L, releasing the autoinhibition and stimulating the methylation of nearby CpG sites on the target...expression off. Plus, DNA methylation is a long-lasting repressive marker, allowing the team to develop...

...GFP-like probes by supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional...mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological Studies) Multiple uses: From...applications.  There is a whole set of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names...occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For...developed based on mIFP6. Learn more about the characteristics, performance, and applications of near-infrared...

...article is to provide enough background for you to distinguish the features of any common lab strain and determine...them in 1885. E. coli are mainly found in the intestinal tract of animals. There are many different naturally...hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated... Hte   High transformation efficiency deoR constitutive expression of genes for deoxyribose synthesis...strains; however, these tables are by no means exhaustive! For a more comprehensive list and additional...

...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...downstream characterization of, isolating 2 or 3 distinct clones to perform key experiments on is a good...principle holds true - the higher the number of distinct editing events, the lower the likelihood of an...transduction control, a different gRNA would have distinct off-target sites, eliminating its effectiveness...

...chemical input to an RSM drives expression of a distinct large serine recombinase that recognizes its own...demonstrates an example of a register that enters distinct states when treated with different combinations...designs, we have observed that certain GRSMs may still not perform as expected, perhaps due to idiosyncrasies...the same gene regulation program. We recommend testing multiple register designs for the same gene regulation...described in the literature (5, 6) and may be substituted for one another in an RSM. The predictability...

...was fascinated by CRISPR and rushed to test it. I still remember the day, March 16, 2014, that I got my ... in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...transmission of the transgene, this technique is still in need of improvement. Also when working with CRISPR... discovered in an obscure bacterium and that we still have much to learn from biology in all its forms...editing.     Haoyi Wang is a professor at the Institute of Zoology, Chinese Academy of Sciences, and he...

... transcription factors, or when the gene is constitutively-active or a dominant-negative mutant. Reducing... TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely seamless...researcher may subsequently kill off any cells that still contain the donor plasmid after excision by PBx,... technology using a site-specific nuclease to stimulate homologous recombination and Footprint-Free™ removal...and I. M. Verma, “Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from...

...Fellow in the Church and Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is...CRISPR during his postdoc 12:01 - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why .... 13:22 - 22:07 : Alex's projects at the Wyss Institute - New CRISPR tools and the importance of moving...26 - 33:08 : Plans for future work at the Wyss Institute. 33:09 - end : How Alex hopes to help Addgene ...

...far? Do you have any suggestions for this year’s teams that may be procrastinating choosing their costume...these. Addgenies have been organizing Halloween festivities for years – searching our photo archives I found... ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, ...

...service and is ready to use out of the box. We estimate that providing cloning grade DNA could save scientists...Plasmids offered as cloning grade DNA As we begin testing out this service, we’re starting with 210 plasmids...molecular biology experiments such as cloning, digestions, PCR etc. and/or directly long term storage. ...service in the future using the Cloning Grade DNA Suggestion Form.  Additional resources on the Addgene blog...

...our in-stock service: ddPCR, purity testing, and sterility testing, plus viral genomic sequencing to confirm...webpage to get started with your browsing and requesting. If you’re curious about the service, read on...on Request AAVs are a big deal, so if you have questions, we have plenty of information available for you...

... service, AAV Packaged on Request  has seen a fantastic response from the research community. Customers...minBG-jRGECO1a-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14360:...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14361: ...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...

...science policy career and this seemingly simple question is a moving target. There are a multitude of options...policy? I’ve intentionally started with the ‘who’ question because the science policy community is the best...start. What is science policy? THE IMPOSSIBLE QUESTION. Science policy is the work you do to creatively... I would argue that this is the most important question that you can explore. The answer will help you...interviews or essays. Note: the answer to this question may change over time and that’s perfectly normal...

...hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ... ́-phosphate to the ligation). The structural/mechanistic reason for this differential is not known for...Despite this higher fidelity, Taq DNA Ligase can still detectably ligate many T:G, T:T, and A:C mismatches...In this method, substrate pools were prepared consisting of one target (template) strand and four upstream...Reaction Temperature Calculator can be used to estimate an appropriate incubation temperature when using...

...development of phage-based therapeutics. After a brief stint at the venture capital firm, Flagship Ventures, ...opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens in food products prior to Sample ... from developing therapeutics to developing a diagnostic? 36:53 - 38:00 : As an entrepreneur, is it more...Read Sample 6's Paper on Bacteriophage-based Diagnostics Additional Resources on the Addgene Blog Check...

...plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries. We continue to ship...plasmids, analyze QC sequence, answer scientists' questions, manage our website, and continue to improve the...plasmids. The partnerships and collaborations with institutions and tech transfer offices that facilitate MTAs...improve, so thank you. 500,000x Thank You! Have a question? Email: [email protected] Call: +1 (617) 225...

...to be used as research tools is particularly interesting. Although humans have known about bioluminescent...bioluminescent organisms and their characteristic radiance for some time – there are records describing them dating...ring that glows with a faint green light. To investigate this green glimmer, Shimomura collected many,... Roger Tsien resulted in much of our current mechanistic understanding of GFP’s function. His lab described...

...microscope designs may further exploit the unique characteristics of fluorophores to block out of focus fluorescence...for a new sample I always start by asking two questions: 1) Is the sample dynamic or static? 2) Is the...the sample thin (< 15 μm) or thick? These questions will place each sample into one of four categories ...blurry out-of-focus light. The most difficult characteristic to capture in these types of samples is their...from a well-established microscopist at your institution when heading into a new set of experiments. ...

...proteins, so you can't distinguish the different proteins from each other. To distinguish different target ...inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...fluorophore? Strong brightness makes it easier to distinguish a signal from noise; therefore, it is recommended...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

...digital PCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...

...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...representing core nodes that, when over-expressed, constitutively activated or inactivated the pathway. “The ...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs...

...Maintaining Institutional Knolwedge and Continuity - SITN's key technique for retaining institutional knowledge...mosquitoes, allergies, & more! We briefly answer the question, will the effects of climate change make put Cambridge...junior and one senior.  To learn more about the STIN's podcast, email [email protected]    For more...

... Twitter.” This paper addresses the following questions about scientists on Twitter: who are they? What...they are based on the US Occupational Employment Statistics (OES), but international Twitter users were included...orange)  scientists largely cluster by discipline, suggesting that scientists tend to follow scientists within...out the Top 50 Science Stars on Twitter Want suggestions on how to use social media as a scientist? Check...

...                           National Institute for Physiological Sciences Broad Institute                                       ...Hospital                           California Institute of Technology             Johns Hopkins University...Academy of Medical Sciences University of Texas at Austin University of California, San Francisco  University...

... the panelists were interesting enough to make up for the somewhat naïve questions. During the panel, ...their seminal, cooperative paper in Science.  Interestingly, this project started with their labs studying...for billions of years (…) Bacteria are really sophisticated”.     New to CRISPR/Cas9 Technology? Learn ...

...14] Hepatocytes [15] Pancreatic β-cells [16] Intestinal tissue [17] Lung epithelial cells [18] Red ...approaches differ in several ways. Below is a table listing some of critical differences (adapted from Zhou...Zhou and Melton, 2008, [43]): Characteristic Reprogramming+ Transdifferentiation in vivo or in vitro...differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 2011. 470(7332): p. ...37. Merrell, A.J. and B.Z. Stanger, Adult cell plasticity in vivo: de-differentiation and transdifferentiation...

...property by including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is flanked... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...sites. To introduce a gene of interest into the destination plasmid, recombination sites must first be added...

...composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly species that jump... is a lot of excitement around using sdAbs in diagnostic applications. For example, fluorescently and ...generate new sdAbs. There has also been some interesting work done developing transgenic mice that express...S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https...

... fastest way for us to release your plasmids is still for you to notify us as soon as your article is ...a project isn’t working out as planned, but you still hope somebody will find the plasmids useful, we’...article, a preprint, or without any paper at all. Posting preprints and openly sharing materials are both...think. We appreciate your feedback. If you have questions about depositing, check out our website, Help ...

...mitigating noise generated by off target digestion. After MNase digestion, fragments are released from nuclei... DNA around the target protein. This targeted digestion is controlled by the addition of calcium, which...measure of DNA binding sites in combination with existing ChIP-seq data sets, of which there are many readily...

... some special characteristics of scientists in the work place as described by Sebastiano Massaro in his...some of these common pitfalls of management and still not get the most from your team. It is pretty clear...learned to get results with the carrot, and not the stick.     This is the first in a 5 part series on Introduction...

...are on the right track if they start asking you questions. Remember that people like a good story and science... this case you – on a quest to ask and answer questions and hopefully discover something new. Science ...out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ...

...Makarova et al. grouped the systems into three distinct types and subtypes using polythetic classification... not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between...viral anti-CRISPR genes. These data highlight an interesting "arms race" between phages and prokaryotes; the... and further develop the CRISPR/CAS toolkit by posting generic lab protocols, providing tips from experts...

...University, and the Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition...Nathan Shaner and colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...

... mountain – with many routes up and only one destination – a career in the sciences is more like an intergalactic...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual... weird and rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination...with things like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance...

...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...were unable to deduce the mechanism responsible we still believe that these results will be beneficial to...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...

... have the potential to provide improvements to existing technologies based on polymerase processivity....stable, highly soluble, and non-immunogenic yet are still just as specific to their target as regular antibodies...podcast segment Steven Carr’s lab at the Broad Institute focuses on proteomics: developing and applying...analysed. In this work, the authors specifically investigated protein binding at the TERT gene, but this method...

... replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies...Patrick says researchers can ask themselves a few questions to get started: Will the plasmid be used exclusively...that plasmids with low to medium copy numbers can still express massive amounts of protein given the proper...cells have not started to die off. Have any questions? Need something clarified? If so please, post ...

...tips for scientists working at home, and you will still find this useful. Now that we’ve been doing this...just need to know you well enough to ask good questions and hold you accountable for your goals.  I’ve...interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation builds...edit everything you’ve tried to draft, but you’ll still be practicing and getting familiar with the process...