We narrowed to 731 results for: Sis;

1. Phage Directory: From Phage Therapy to a Repository of Phage Information

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   Blog Post

   ...Twitter to find phages to treat an antibiotic resistant bacterial infection, she shared that tweet with...

2. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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   Blog Post

   ...are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and S-CHIME, we discovered that...

3. Six Spooky Science Stories and Halloween at Addgene

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   Blog Post

   ...encode P450 enzymes that mediate steroid hormone biosynthesis in Drosophila melanogaster." Molecular and cellular...

4. Science as Art

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   Blog Post

   ...motivated to get data, publish in time, defend the thesis, etc. Photography is critically important in many...

5. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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   Blog Post

   ...require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1 cleavage assay, sequencing...

6. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

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   Blog Post

   ... Be?” In this excellent essay Acclimatrix, an assistant professor at a research university in the northeast...

7. Scientific Sharing in the Time of COVID-19: Databases and Resources

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   Blog Post

   ...researchers in other disciplines (bioinformatics, image analysis, AI…).  How to put the wider scientific community...

8. What's New in CRISPR - March 2020

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   Blog Post

   ...expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian transcriptional repressor...

9. Working 9-5: an Addgene Co-op

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   Blog Post

   ...uses something called “core hours”. Core hours consist of the time between 10 AM and 3 PM, when most meetings...

10. New Norepinephrine Indicators: nLightG and nLightR

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    Blog Post

    ... Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References and resources...

11. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

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    Blog Post

    ...imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the...

12. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post

    ...chromosomal translocations or chromosomal shattering (chromothripsis). As this study joins the pantheon of useful...

13. Future of Research Conference - Remarkable Opening Session

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    Blog Post

    ... away from PI driven basic research, there is resistance to change, public support is weak, government...

14. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

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    Blog Post

    ...nature, with the ultimate goal to improve the diagnosis of disease, and cancer in particular. They recently...

15. 9 Tips to Achieve Success in Academia

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    Blog Post

    ...aware of careers outside of academia. How do PIs assist their mentees who have goals outside of the university...

16. Handling Plasmids from Addgene - Purifying Plasmid DNA

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    Protocol

    ...like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA Purification Streaking and ...purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the following solutions...phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom phase...

17. What's the Best Way to Elute and Store Your Plasmid DNA?

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    Blog Post

    ...mechanism called depurination. In depurination, the hydrolysis of the glycosidic bond leads to the release of...

18. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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    Blog Post

    ...2011) Power tools for gene expression and clonal analysis in Drosophila. Nature Methods 9:47–55 . https:...

19. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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    Blog Post

    ...configuration. In addition, D10A editing efficiency is consistently higher than that of H840A, especially with smaller...

20. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    Blog Post

    ...through our Snapgene powered maps and sequence analysis tools.     The main ways we use SnapGene for...

21. Hot Plasmids and Viral Preps - July 2021

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    ...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease....

22. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    Blog Post

    ... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

23. CRISPR 101: Drug discovery and CRISPR

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    Blog Post

    ..., fast, and easy. In cases where the mutational basis of a disease is known – repeat expansion, gene knock...

24. Cellosaurus: A Cell Line Information Database

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    Blog Post

    ...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...

25. Hot Plasmids - October 2022

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    Blog Post

    ...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...

26. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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    Blog Post

    ...Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure. ReFigure...

27. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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    Blog Post

    ...from home and looking for a job.” But Caitlin’s persistence paid off: “I spent about 6 months just editing...

28. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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    Blog Post

    ...performance data, protocols, and even certificates of analysis.” Han’s biggest piece of advice for those looking...

29. Understanding GPCR Signaling Using TRUPATH

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    Blog Post

    ...(Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and...

30. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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    Blog Post

    ...for the user. This approach serves to maintain consistency with published screen interpretations and allows...

31. Bricking Science: Portraying Scientific Reality Through LEGO

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    Blog Post

    ...is the most approachable for LEGOs. Stop motion consists of taking many pictures, one for each position...

32. Running for Rare Disease, Running for FOP, Running for AJ

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    Blog Post

    ...put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined...

33. Management for Scientists: Giving Feedback

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    Blog Post

    ...Feedback is help and it should result in authentic assistance for the recipient to become more effective and...

34. Choosing Your Perfect Empty Backbone

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    Blog Post

    ...with the proper selection marker or antibiotic resistance. Next, consider the expression level of your ...

35. Advice for Choosing a Research Project

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    Blog Post

    ...to use to reach these milestones? Can you get assistance from people who have the required expertise to...

36. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post

    ...before the advent of techniques like PCR and DNA synthesis. Most molecular biologists bought the pricey stuff...

37. Virus Protocol - Generating Stable Cell Lines

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    Protocol

    ...lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...

38. Plasmid Cloning by PCR (with Protocols)

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    Protocol

    ...molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the primer assist with restriction...selecting restriction sites, you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer , to...

39. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    Blog Post

    ...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

40. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    Blog Post

    ...fluorophore, or APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP...