We narrowed to 794 results for: Tec

...measurements such as patch clamp recordings. However, technical difficulties such as small fluorescence changes...QuasAr-mOrange and MacQ-mCitrine. All of these sensors can detect action potentials in worms with fluorescence changes...

...limitations. First, FRET signals are often difficult to detect, and thus quantify, due to small fluorescence changes...induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours, a time consistent...

...particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI.   Additional...resources Find protocol videos to teach basic lab techniques Plasmid Land: The plasmid cloning game ...

...viral particles per cell, you may not be able to detect AAV-mediated gene expression in a target cell. ...confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production...

...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020...

...conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...you stay connected. If possible, test out the technology before the conference begins.“There were some...

... eat, and rest. Try the Pomodoro Technique or the Flowtime Technique. Co-work virtually. With co-working...

...responsiveness, they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and...iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and beyond. These...

...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9... for microscopy by: Susanna Stroik Microscopy techniques have been moving at the speed of light over the...

...contrast to recent genome engineering technologies, stem cell techniques are neither fast nor inexpensive....

...Culture by Gibco Whenever I start to learn a new technique, I try to look for a resource to help me. I’m .... When I started learning proper cell culture technique, I tried to find an app that could help me with...

...thought that they function as a kind of armor to protect their host from predation (Petroni et al 2000)....the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest ...

...fluorescent proteins whose spectra overlap, there are techniques, like spectral unmixing, which can be used to...green and red proteins are generally the most detectable and so should be used to tag your least abundant...

...introduction of DNA into bacterial cells); however, the techniques and reagents vary. Plasmid transfection into ...positive selection; however, negative selection techniques can be used in conjunction with positive selection...

...With increasingly sophisticated visualization techniques it is possible to follow the interactions between...microvillar search and stabilization during ligand detection by T cells." Science 356.6338 (2017): eaal3118...

...patient’s tumor sample, use cutting edge genomic technologies to map the mutations that are present, and use..., these early pilot studies have demonstrated technical feasibility and proof-of-concept. If such approaches...

...Without these scientists and the support of their technology transfer offices, Addgene would not be able to...educational materials, and provides viral vector technical service (details about virus production protocols...

...infrared fluorescent protein tag which let the team detect its co-localization with a membrane-localized mCherry...about using the secondary nanobody toolbox for immunodetection Resources on Addgene.org Find nanobody expression...

...into bacteria or eukaryotic cells is a common technique in molecular biology and scientific research. ...page, which has both protocols and videos for techniques in basic molecular biology, plasmid cloning, ...

...use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...

...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a long gene (>...

...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

...also activating or inhibiting gene expression, detecting neuronal signaling, or manipulating any other ...Others have noted a lack of transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe...

...restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The other component...Addgene Blog Keep up with the Latest Cloning Techniques Learn to Troubleshoot Your Sequencing Results...

...For insertions, it’s possible to use the same technique just using a large primer known as a megaprimer...Addgene Blog Read about Other Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to...

...overview of general molecular biology knowledge and techniques. If you are interested in reading more, you can...Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated list of Bacterial...

...involved “plates,” and our scientists and lab technicians always knew from context what a “plate” was. ... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...

...Tools for studying bioelectricity Dyes and other technologies are now available for fluorescent reporting ...synthetic bioengineering." Current opinion in biotechnology 52 (2018): 134-144. PubMed PMID: 29684787. Oviedo...

...the steps you will have to take. Decide which technique you are going to adopt (i.e. restriction cloning...Find how-to videos for molecular biology and lab techniques ...

...PubMed PMID: 29043675.Grefen C and MR Blatt. Biotechniques 2012. PubMed PMID: 23066669.Hecker A et al, ...Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify novel...

...exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective small molecule...author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet.   References...

...laboratory situation or tissue collection method, so biotechnology companies have marketed several products that...These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen, are ammonium sulfate-based solutions...

... Cas14 makes it a promising detection tool for rare, often hard to detect variants such as single nucleotide...

... the open windows in the middle of your talk. Technical difficulties turn your slide deck into a chalk...everyone! Everybody makes mistakes, encounters technical difficulties, and runs into unexpected stumbling...

...Proteins with a Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff...growth factor I from culture medium of E.coli. Bio/Technology. 1987, 5:379-382. Bornhorst JA and Falke JJ. ...

... will not recognize a Fab fragment. In order to detect a Fab fragment, it either needs to be directly ...identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem Cytochem...

...error rate of current NGS approaches allows for a detection limit of roughly 0.1%. Thus, rare, pre-existing...prior to treatment, there are small (previously undetectable) populations of cancer cells that are resistant...

...control. But even with the advent of CRISPR/Cas9 technology, inserting large tags into a precise location...plasmid into the cells we use electroporation, a technique that briefly destabilizes the cell’s outer membrane...

...hexon proteins. The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid... assays are commercially available for faster detection of RCA. 2nd Gen AdVs were designed to have increased...

...10.1021/acssynbio.9b00395 O’Brian, SP, et al., Biotechnol J.  2014. https://pubmed.ncbi.nlm.nih.gov/24390935...as time-lapse imaging or with high light power detection of molecules.  Lee, J. et al., Science Advances...