We narrowed to 911 results for: Tro;

...favorite gene. But have no fear - I’m here to introduce you to a simpler, yet more effective, CRISPR-based...Ming G-L, King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA...

...activation can occur through fusing dCas9 with VP64, a strong transcriptional activation domain. Guided by dCas9...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https:...

... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems added another ... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the cleavage...

...who spend most of their time moaning about their troubles, Silent or unresponsive clams, who seem to ignore...Indecisives, who keep changing their minds and have trouble making decisions. In my work as a thesis coach...

...ll need to produce your viruses in-house.   Introducing the systems There are many ways that viruses ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

...apart is the way they generate their color: they strongly absorb visible light to give colors in ambient...genome, it is not expressed as it is under the control of a rhamnose-inducible promoter. The resolution...

...not that I would know about this… A no template control (NTC) should always be included. While the physical...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

...protein’s structure into experimentally determined electron density, have been known to say something silly...educators. Using these hands-on tools, educators introduce their students to this otherwise invisible molecular...

...used to tag endogenous genes and simultaneously introduce a selection marker for screening modified strains...with a Cas9 expression vector. They can also be introduced directly into a Drosophila S2 cell line stably...

...research moving. Below, you’ll find some great introductory resources. As you dive deeper into Drosophila...TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock...

...Importantly, all of these assays are performed in vitro. Assays to detect FP dimerization in in vivo cellular...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...use of viruses allows much better spatiotemporal control over labeling methods as compared to transgenic...

...False, embed_button=False, autoplay=False, hidden_controls=False, loop=False, muted=False, width='600'... impacted. The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect...

...but the addition of a high concentration of a chaotropic salt (guanidine hydrochloride for the plasmid... glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two...

...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ...

...can occur in two different orientations, but is strongly biased towards T-RL, where the R end is proximal...coli (Klompe et al., 2019). Recently, the lab introduced a streamlined expression approach (Fig. 2A), ...

...anyone can find enhancers with Quilt. Seek and destroy enhancers with CRISPR Armed with the genome math...ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as HIV. By design, our lentivirii...

... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems has added another... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the cleavage...

...glycerol stocks, prepped DNA ready for our quality control processes - all of these samples have the 2D barcodes...along the way involves good aseptic technique and controls to ensure that nothing is contaminated. We use...

...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

...years of experience in my job, I was willing to introduce myself to someone new and ask for help. That was...confidence to meet someone new, as they’d been introduced to several people by their mentor, as well as...

...defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). The reversion of this...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...measurements. Extra care should be taken so as not to destroy the donor molecule. Fluorescence lifetime imaging...

...alongside standard surface labels. Here, I will introduce you to a few useful labeling and staining techniques...Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

...If you read my eBook and it helps you, please introduce yourself to me by email or in person. If you feel...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

...evident that widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR... need for thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the...

...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...women), visibility (how to get it, especially if introverted ), and networking (how to get people to remember...

...exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or absence of dNTPs. Secondly...assembly a ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in...

...Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools...

...Structure-guided directed evolution If you desire a bit more control, structure guided directed evolution might be more...evolution, all while maintaining a higher degree of control and efficiency! Screening mutant libraries After...

...constructs were designed to be packaged into a retrovirus for infection of host cells. The fluorescent ... is composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase...

...Research requires dedication and effort, so building a strong understanding of what you’re getting yourself into... what I don’t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what...

...culture spilled immediately. Water baths, liquid nitrogen containers, reagent bottles and other sources ...Mycoplasma Infections from Continuous Cell Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290...

...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and everything...policy training programs and fellowships have a strong focus on early career researchers who have decided...

...specify and correct single-base mutations without introducing DSBs at all. Base editing to the rescue! Base...editor. Dotted red arrows indicate the change introduced to the opposite strand following base editing...

...immunocytochemistry, and flow cytometry. It's a great introduction to — or quick refresher on — these common antibody...

...nomenclature to popular cloning techniques and troubleshooting tips. The fourth edition has been fully updated...

...some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this...

...scientists everywhere. In it, you can find an introduction to viral vectors and their elements; resources...

...resources on the Addgene blog Antibodies 101: Introduction to Antibodies New Antibody Animation ...

...Please check out the updated guide for a great introduction to what CRISPR technologies have to offer. Happy...

...Aavery!       Original post: In December, we introduced you to Blugene’s new virus friend (they/them/...

...McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author: Lila WittTips for Getting a Faculty ...

...We first “went viral” in 2016 when we introduced the viral service, and now we’re “going viral” again...

...Addgene gave to me: ways to use optogenetics to control CRISPR editing.  On the fifth day of CRISPR, Addgene...

...Science in the News, Part 1 Podcast breakdown Introduction to Vini, Amy, and SITN The Most Difficult thing...

...goals, both from the Baker Lab and others. The introduction of de novo designed proteins also helped researchers...

...sensorRUBY-Red Siliques: Guest blogger Andrew Willoughby introduced us to RUBY-red, a bright red pigment reporter...

...many steps in the viral production and quality control process to improve both the yield and quality of...

...protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new...