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We narrowed to 916 results for: Tro;

Showing: 651 - 700 of 916 results

  4. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region will ...  Susanna Stroik...

  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...sites on the target protein they tend to have a strong signal and to work in a variety of applications...Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283...

  11. Lab Automation at Addgene

    Type
    Blog Post
    ...into the robotics spirit include: Start using electronic multichannel and single channel pipettes. These...by one. It seems very simplistic and it isn’t electronic but it is one of the most helpful machines I ...

  12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems added another ... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the cleavage...

  14. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...use of viruses allows much better spatiotemporal control over labeling methods as compared to transgenic...

  15. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...ll need to produce your viruses in-house.   Introducing the systems There are many ways that viruses ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

  16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...not that I would know about this… A no template control (NTC) should always be included. While the physical...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...protein’s structure into experimentally determined electron density, have been known to say something silly...educators. Using these hands-on tools, educators introduce their students to this otherwise invisible molecular...

  19. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...used to tag endogenous genes and simultaneously introduce a selection marker for screening modified strains...with a Cas9 expression vector. They can also be introduced directly into a Drosophila S2 cell line stably...

  21. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems has added another... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the cleavage...

  23. Tips for arabidopsis transformation

    Type
    Blog Post
    ...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ...

  25. DNA Purification Without a Kit

    Type
    Blog Post
    ...but the addition of a high concentration of a chaotropic salt (guanidine hydrochloride for the plasmid... glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two...

  28. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...measurements. Extra care should be taken so as not to destroy the donor molecule. Fluorescence lifetime imaging...

  29. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). The reversion of this...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

  31. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

  32. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...alongside standard surface labels. Here, I will introduce you to a few useful labeling and staining techniques...Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

  33. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...If you read my eBook and it helps you, please introduce yourself to me by email or in person. If you feel...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

  34. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...evident that widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR... need for thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the...

  35. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...women), visibility (how to get it, especially if introverted ), and networking (how to get people to remember...

  37. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools...

  38. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...constructs were designed to be packaged into a retrovirus for infection of host cells. The fluorescent ... is composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase...

  39. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Research requires dedication and effort, so building a strong understanding of what you’re getting yourself into... what I don’t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what...

  40. Finding Your Science Policy Path

    Type
    Blog Post
    ...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and everything...policy training programs and fellowships have a strong focus on early career researchers who have decided...

  42. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...specify and correct single-base mutations without introducing DSBs at all. Base editing to the rescue! Base...editor. Dotted red arrows indicate the change introduced to the opposite strand following base editing...

  43. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ...immunocytochemistry, and flow cytometry. It's a great introduction to — or quick refresher on — these common antibody...

  44. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...nomenclature to popular cloning techniques and troubleshooting tips. The fourth edition has been fully updated...

  48. CRISPR Guide Update

    Type
    Blog Post
    ...Please check out the updated guide for a great introduction to what CRISPR technologies have to offer. Happy...
Showing: 651 - 700 of 916 results