Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,092 results for: addgene

Showing: 651 - 700 of 1092 results

  1. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...new X-CHIME systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment....1038/s41467-019-09656-2 More resources on the Addgene blog CRISPR101: Validating Your Genome Edit How...Genome Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions...

  3. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau, R.W., Lee-Gosselin, A....PubMed PMID: 29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...

  5. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...Editing the Epigenome (plus bonus podcast with Addgene Advisory Board member Alex Chavez) Day 11: Pooled...

  6. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...scientistswhotweet. And don’t forget to follow us @Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C....scientist? Check out this post. Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...

  7. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration...fluorescent molecule   Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

  8. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it...PMCID: PMC4252608. Additional Resources on the Addgene Blog Sign Up to Receive the Fluorescent Protein...about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein Empy Backbones Visit...

  10. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers...journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped...

  11. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...product for isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids...few. Have you used these tools in your own lab? Addgene would love to hear from you, our community, about...

  12. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...within an organization, there can be variance. At Addgene, our content team, where writing is a core skill...updating this post, I took an informal poll of Addgene hiring managers. All of them stated that a good... in May 2024.     Additional Resources on the Addgene Blog Learn to Develop Your Professional Network...

  13. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...your work. Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x cysteine...29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug, H., Görlich...PubMed PMID: 23495938. Additional Resources on the Addgene Blog DIY DNA ladders from Penn State University... protein expression Additional Resources on Addgene.org Find more nanobody expression plasmids here ...

  14. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...and Success in Management Resources at Addgene.org Addgene's Management Team ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...Business School Blog Additional Resources on the Addgene Blog Management for Scientists Series What Makes...

  15. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ... as Customer Support and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team...former Customer Support and Operations Manager at Addgene and is interested in nonprofit management and international...development.       Additional Resources on the Addgene Blog Get tips on writing a good cover letter Resume... careers Learn about mentoring Resources on Addgene.org Check out our careers page Watch our career ...

  16. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    ...plasmids and PHP.eB viral preps are available from Addgene. References Holmes, Christina, and William L....PMCID: PMC5088052. Additional resources on the Addgene blog Learn more about PHP.eB, and PHP.S, a peripheral...AAV, and much more! Find ready-to-use AAV from Addgene or check out protocols for packaging your own virus...for gene expression in mammals. Resources on Addgene.org Search for AAV vectors to use with your research...

  17. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Resources on Addgene.org Find protocols of molecular biology techniques Find plasmids at Addgene Make your...@rory_macneil.     Additional resources on the Addgene blog Find tips on choosing an electronic lab notebook...your work more accessible by depositing at Addgene ...

  18. Tips for arabidopsis transformation

    Type
    Blog Post
    ...Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing an expression system Choosing an appropriate...21 September 2018). Additional resources on the Addgene blog Engineering the plant genome using CRISPR...resource page Deposit arabidopsis plasmids with Addgene ...Using arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource...

  19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...Resources on the Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about...Find pooled libraries available at Addgene Find CRISPRko (Brie), CRISPRi (Dolomiti), and CRISRPa (Caprano...genome-wide screening using CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here Find...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...and PNS Resources on Addgene.org Find the Spaghetti Monster probes at Addgene Browse all AAV vectors...University. Find the Spaghetti Monster probes at Addgene Uses of smFPs 1. Multi-channel connectomic tracers...PubMed PMID: 18057027. Additional Resources on the Addgene Blog Rabies and neuronal tracing Retrograde AAV...

  21. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...in the dark compartment (Howerton at al. 2014). Addgene has several recombinase-expressing ready-made AAV...PMCID: PMC4299443.  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  22. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...standard FPs. The moxFPs are now available through Addgene Many thanks to our guest bloggers Erik L. Snapp...Central PMCID: PMC2150754. Resources Avaliable at Addgene Read Our Blog Post on Protein Tags Read Other ...Protein Pages Find the mOX Fluorescent Proteins at Addgene   ...

  23. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...number to the capsid. Most vectors provided by Addgene are pseudotypes using the genomic structure of ...system. Table 2: Availability of AAV serotypes at Addgene. Serotype In-stock viral prep Packaged on Request...capsid is a good option for your experiment? Use Addgene’s AAV Data Hub to find experimental reports on commonly...10.1128/jvi.00895-06  Additional resources on the Addgene blog Viral Vectors 101: An Introduction to AAV...validation data after your experiment! Thanks to Addgenies Brian O’Neill and Ina Ersing for contributing ... Vectors 101: Systemic Capsids Resources on Addgene.org AAV Guide AAV Data Hub AAV Packaged On Request...

  25. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Additional resources on Addgene.org CRISPR guide CRISPR protocols by Addgene depositors gRNA design software...further consolidate these FAQs, Le Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the ...homology of the protospacer followed by PAM. See Addgene's CRISPR resources page for gRNA design software...scientific community and sharing these resources with Addgene. Thank you, Le! This blog post was updated to include....cell.2013.08.022  Additional resources on the Addgene blog CRISPR Cheat Sheet Build Your CRISPR Vocabulary...

  26. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...plasmids, they don’t go to Wikipedia; they go to Addgene.”-Ryan Mehl, Professor, Oregon State University...

  27. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...experiment. Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors? Check ...the viral vector information that's out there, Addgenie Leila Haery has summed up some of the most important...

  28. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...10.1177/38.7.2355173  Additional resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence...

  29. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...integration, transposon, and many more! Check out Addgene’s catalog to find trapping tools for your next experiment...Plasmids 101 e-book Additional resources on the Addgene blog Plasmids 101: Using Transposons in the Lab....1105/tpc.12.7.1007  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids...

  31. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...Reading, & Organizing Write for the Addgene Blog Resources on Addgene.org Check out Our Revamped Protocol...100percentSDS.     Additional Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific...

  32. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...that can be used in research or for therapeutics Addgene has compiled various educational resources to facilitate... facilitate plasmid use in the lab. Addgene’s Molecular Biology Reference includes information about molecular...

  33. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...communications efforts.  Scicomm definitions (as used by Addgene)    Science communication, or science writing, ... you next time!  Additional resources on the Addgene blog 10 Ways to Share Your Science Science Communication...

  34. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ... References and Resources More resources on Addgene’s blog Antibodies 101: Monoclonal Antibodies Antibodies...

  35. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...PMCID: PMC6092020. Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  37. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...CellTag, you can find the CellTag libraries at Addgene. The Morris Lab's website also has several useful...bioRxiv (2019): 335547. Additional resources on the Addgene blog Learn more about induced pluripotent stems...cell dynamics in model organisms Resources on Addgene.org Visit our stem cell page Find plasmids for reprogramming...

  39. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... currently an Outreach Data Curation Intern at Addgene. She is also a graduate student at the University...PMCID: PMC3207894. Additional Resources on the Addgene Blog Plasmid Cloning by PCR Perform Site Directed...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find Additional...

  40. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...instructions on how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used...natural resistances.  Additional Resources on the Addgene Blog Read other Plasmids 101 Posts Learn How to...Resistance on Our Plasmid Pages Resources on Addgene.org Browse Our Molecular Biology Reference Pages...

  41. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to...presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already...Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein of interest,...PubMed Central PMCID: PMC4380784. Resources on the Addgene Blog Get Tips for Using FRET in Your Experiments...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...

  42. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski,...collection of epitope tag antibodies available through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version... chain and light chain are also available from Addgene!   Figure 1: Experimental validation for...

  44. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...training in a variety of transferable skills. At Addgene we're helping to fill this gap by both helping ... had over lunch are just as valuable! In 2019, Addgene implemented a company-wide New Ideas Survey. Anyone...a-brief-exercise-to-spur-innovation-on-your-team   Additional Resources on the Addgene Blog Transferable Skills Guide: Problem-Solving...from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for example) and let your...

  45. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...microbiology Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids from Addgene ...Plasmids for each of these systems are available from Addgene. If you’ve given any of these systems a try, we...acssynbio.0c00073   Additional resources on the Addgene blog: Browse CRISPR articles from our CRISPR topic...

  46. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...share the plasmids created under this programs via Addgene. New organisms for genotype to phenotype studies...minute) talks so we got to hear from everyone. Addgene is certainly going to have to find ways to tag ...lab? Let us know! Additional resources on the Addgene blog Check out our other model organism blog posts...

  47. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...Bassik CRISPR Knockout Library are available from Addgene! References 1. Morgens, David W., et al. "Genome-scale...Central PMCID: 3652613. Additional Resources on the Addgene Blog Learn to conduct genome-wide screens with...CRISPR Pooled Libraries currently available from Addgene Other Resources Watch Dr. Michael Bassik’s lecture...Guide to All Things Lentivirus Resources on Addgene.org Check out this guide to pooled plasmid libraries...

  49. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...open access journals.   Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching ...Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...

  50. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...
Showing: 651 - 700 of 1092 results