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We narrowed to 967 results for: ARC

Showing: 261 - 280 of 967 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Plasmids from:  March (Q1) | June (Q2) | September 2016 (Q3) | December 2016 (Q3) MARCH 2016Enhancer activity... much literature searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore... states. Alexander Stark and colleagues at the Research Institute of Molecular Pathology have developed... library     CRISPR pooled libraries enable researchers to screen the entire genome for genes that regulate...newly available PRESTO-TANGO kit which allows researchers to interrogate the druggable human GPCR-ome using...utility of this platform for drug discovery. Researchers can use plasmids from the PRESTO-TANGO kit directly...-disciplinary Integration of Design Automation Research lab Moduclar Cloning (CIDAR MoClo) Parts Kit. ...

  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...various light-controlled CRISPR tools available to researchers - some readily found at Addgene. Shining light...position to activate gene transcription. The researchers tested a variety of combinations to optimize ...pattern during blue light exposure (470nm), the researchers showed that expression of the human ASCL1 gene...cells to induce expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after ...cycled on-off-on. Using a slit photomask, the researchers also demonstrated the ability to spatially control...-mediated CRISPR labeling to study chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins...can potentially alter the genome and chromatin architecture, it is useful to regulate the time in which ...

  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...whole-genome sequencing becomes more relevant (Garcillán-Barcia et al., 2023). In this post, we’ve grouped...https://doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F...experimental tools. They certainly are very handy in research, but we’re really borrowing these tools from Mother...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA...plasmids can be dangerous, they also provide researchers with new means to deliver DNA and other molecules...tend to be small and poorly studied, but some researchers have suggested they may have relevance to human...natural plasmids and their functions has enabled researchers to develop and deposit the many plasmid tools...

  4. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...Addgene to accelerate their research. Read on to learn how these researchers plan to use plasmids from ...Genomics Institute to provide plasmid grants to researchers working on rare diseases through the BeHEARD ...from Addgene to advance their research on diseases of glycosylation, arginine mutagenesis, and neural development...She leads the Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences (UCIBIO)... (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals and Patient... Colorado, USA. The primary focus of his lab’s research is the mechanisms by which proteins assemble into... a professor at the University of Buffalo. His research focuses on understanding the structural determinants...

  5. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs...for your research during this holiday season and we look forward to seeing what awesome research you and... offering to share their stories – about their research, their job hunts, and their tips for experiments...you have any questions or if you have a topic, researcher, or technology you would like us to feature in...looking for guest bloggers who want to share their research with the community, so email us at [email protected] Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick Will You Be My Mentor? Finding and Asking...

  6. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...increasingly popular in fundamental and applied research since their first use in the early 90’s to genetically...inefficient at infecting non-dividing cells and so researchers set out to create a better, safer system. Unlike...than the first one and can be used routinely in a research laboratory. The risk of generating RCL is low ...technique, there’s always a small chance that a researcher could infect him or herself with the oncogenic... generation lentiviral vectors can be used for research and for clinical purposes, however improving the...safety of these vectors is still an active area of research due to the possibility that mutation or recombination...lentiviruses with human tropism can still infect the researcher and insert into her/his genome - this is a biosafety...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... large-scale profiling of many GPCRs, Mikel Garcia-Marcos’ lab recently developed a collection of BRET-based.... E., Zhang, H., Layne, M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-...biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots By Emily...New recombinant antibodies for neurodegeneration research   By Ashley Waldron We recently added two antibodies...against important targets for neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both ...hope these will become valuable tools for your research! If they do (or if they don't!), please consider...consider sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  ...

  8. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...inhibition of action potential generation. Archaerhodopsin (Arch) is activated at a similar spectrum as HR... the first time in the history of neuroscience research we have a technology that allows us to show causality...discarded but clearly we are in an era of neuroscience research where optogenetics will be a standard tool used...the National Institutes of Drug Abuse Intramural Research Program (NIH-NIDA IRP), with the stated goal of...they can be used is to consult the many primary research papers that have been published at this point....through hydrogen ion efflux; evidence suggests that Arch may more effectively silence neurons than HR. Delivering...construct using stereotaxic surgery is a neuroscience research technique often used beyond just optogenetics....

  9. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage...plasmids every quarter. The “hot plasmids” from last March were quite popular and represent the diversity of...academic job interview including defining your research vision, organizing your vision into a chalk talk...many model organisms people are using for their research. In this blog post, we’ve covered five model organisms...yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics... technique in molecular biology and scientific research. Get an overview of these methods in this blog...fantastic year and happy reading. Best wishes on your research in 2020! Additional resources on the Addgene ...

  10. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...contributed by guest bloggers Marcelle Tuttle and Alex Chavez, researchers at the Wyss Institute for Biologically... thanks to our guest bloggers Marcelle Tuttle and Alex Chavez! Marcelle Tuttle is a medical student at...domain (Fig 1). Two ways to use CRISPRa for your research CRISPRa for specific gene targets CRISPR activation...scale of activation allows for exploratory-based research as well. For example, CRISPRa can be used to find... at Tufts University and former researcher at the Wyss Institute for Biologically Inspired Engineering...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and transcription...

  11. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...  Easily search for plasmids (and more!) The main goal for the homepage was to improve search and browse...redesign, the new search bar is now front and center, with a clear call to action - Search! Along with this...It became clear to us that we needed to improve search and navigation, while also updating brand messaging...Community, sharing, and fun Along with improving search and navigation, we also wanted to emphasize the...the Addgene blog Learn more about Addgene's new search tool Read more about other features of Addgene'...website Resources on Addgene.org Try out the new search on Addgene's homepage ...

  12. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...in full-length publications. As early career researchers, we wondered how we could make the insights gained...around reading through entire papers, perhaps you search google images for specific experimental results...These data are valuable but remain hidden from research papers as they are not included in traditional...study with multiple patients was published, Zika researcher Christa Osuna collated Zika case studies, making... the NIH and Wellcome Trust now accept interim research products (pre-registration, preprints, data, software...micropublications) in grant proposals. ReFigures are one such research product which can convince reviewers of your expertise...recommend sharing ReFigures on your Linkedin and ResearchGate profile. To see how to create ReFigures in a...

  13. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...your favorite search term, i.e. “biology + masters.” For lab-based roles, look for “research associate,”...have masters by using the search term “master’s degree.” Use the LinkedIn search filters to limit results...postings  Look at master’s-level job postings on a job search website like Indeed. Do the job descriptions sound...manuscript and grant preparation, and lead your own research project.  3. Do you prefer being a generalist?...variety of projects and in different fields of research is one of the best parts of my career with a master...master’s. While it's possible to change fields of research with a PhD, a master’s is less specialized so ...

  14. p53 Pathway

    Type
    Collection
    ... Cancer Research p53 Pathway p53 Pathway You May Also Like: Cancer...

  15. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates with homology...

  16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...mechanism of incorporation is not well understood, researchers have been using AAV vectors for gene editing ...iPSCs from ~0.2% to ~1.5%. In more recent work, researchers have combined AAVs with CRISPR systems. Ohmori...an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to deliver CRISPR components...delivery method frees up space in the AAV allowing a researcher to fill it with the repair template. Bok and ...hematopoietic stem cells respectively. Future researchers could apply this technique to efficiently deliver... continue to use and develop AAVs for your own research and encourage to deposit any new AAV tools you...Cancer Center, Georgetown School of Medicine. His research currently focuses on determining the mechanisms...

  17. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...10.1016/j.cell.2020.12.015 Garcia-Beltran WF, Lam EC, St. Denis K, Nitido AD, Garcia ZH, Hauser BM, Feldman...current vaccines differed depending on the variants (Garcia-Beltran et al., 2021). Antibodies from individuals...the P.1 and B.1.351 variants tested. Image from Garcia-Beltran et al., 2021 with permission.   The...quantified in terms of luminescence. Image from Garcia-Beltran et al., 2021 with permission.   Mutations...sera with various pseudotyped virus. Image from Garcia-Beltran et al., 2021 with permission.   Difference...plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope that by sharing the plasmids other scientists...146. https://doi.org/10.1038/s41586-021-03471-w Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller...

  18. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Fluorophores In this post, Luke Lavis from Janelia Research Campus reminds us that, although fluorescent proteins.... To learn how you can use minigenomes in your research, check out this post! R Bodies: Membrane-Rupturing... post, Jessica Polka, formerly a postdoctoral researcher in the Silver Lab at Harvard University, describes...CombiGEM is a technology that can be used to quickly barcode a set of DNA sequences. In this post, Alan Wong... Lab at MIT) shows how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening...Genome Editing CRISPR has revolutionized how researchers make knockouts and other types of genome edits...In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab, describes how you can better...

  19. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...database is to browse or search information in more powerful ways, and the advanced search page simplifies the...contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School. The need ... most indispensable tools in modern biological research.  Any microscopist will tell you that selection...has been very useful over the years, and when researchers come to us for advice on choosing a protein, ...particular FP; this can be done directly using the search bar on the homepage (and at the top of all pages...the process of building advanced multi-parametric search queries.  Looking for a bright, monomeric FP published...guest blogger Talley Lambert! Talley Lambert is a Research Associate at Harvard Medical School. He manages...

  20. The MTA at Addgene

    Type
    Blog Post
    ...agreement that states the terms of use for a tangible research material that is shared between two parties. There... for organizations to make policy decisions on research use agreements. The UBMTA, which is the MTA most...well-understood and accepted by the majority of research institutes.  Pro tip! In most academic and nonprofit...Transfer department is responsible for managing research use agreements like MTAs.  Addgene does a lot ...plasmids at Addgene are shared under the UBMTA, researchers additionally have the option of distributing ...which allows the plasmids to be distributed for research use only to industry-based scientists. Some organizations...OpenMTA, which allows plasmids to be used for both research and commercial purposes. It’s important to note...
Showing: 261 - 280 of 967 results