Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 710 results for: BASE;

Showing: 261 - 280 of 710 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...resistance cassette) in pUC19 based entry vectors, as well as the pGreen-IIS based destination vectors. To ...CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means...ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based chemogenetic technologies After several years...up to six DNA fragments at a time. This method (based on the Golden Gate technology) exploits the ability...reconstitution across synaptic partners) technique based on functional complementation between two non-fluorescent...tags in the FP sequence for separation from Cre-based Brainbow-expressing cells. Autobow plasmids are ...The expression plasmids for a given species are based on the same backbone to permit expression levels...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...is less than half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging...was shown to be effective for CRISPR activation, base editing, and genome editing in mammalian cells. ...had the highest activity for gene activation  and base editing, whereas a different set of mutations (the...

  4. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...recombination of the targeting vector. CRISPR/Cas9-based systems have been developed for generating knockin... for gestation. All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also introduce...in which the conditional recombination can occur based on the cells in which Cre is expressed.  Several...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...resolution for in vivo work. APEX2, a faster peroxidase-based labeling approach, is an alternative option but ...signaling. Limitations of RaPID Like other BirA*-based proximity labeling methods, RaPID cannot distinguish...reported by other research groups that performed BirA*-based affinity purification mass spec experiments. These...

  6. Antibodies 101: Conjugation

    Type
    Blog Post
    ...change, so they are typically used in microscopy-based assays like immunohistochemistry, though they are....  Biotin Biotin is used in a range of antibody-based assays. Biotin itself is not visible or capable ...But hopefully, this introduction provides a good base from which to dive deeper!References and resources...instruments, each other, and your target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...gradient with different concentrations of your protein-based buffer (FBS, milk, etc.) against the same amount...instead of concentrations. Most labs use protein-based buffers, so here’s a quick tip: Keep a bottle of...even give gelatin a shot.  Remember that protein-based blockers degrade fairly quickly, even at 4 °C, so...peroxidase protein (HRP), have a limited dynamic range based on the solution used to expose them. You’ll need...(if available) and note in analysis Plasmid-based proteins: plasmid sequence not accurate Check ...and/or concentration Try a different protein-based buffer (eg, milk instead of BSA) Nonspecific...buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular fractional kit or...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...diffusion rates. Smaller genetically encoded probes are based on cofactor binding proteins as described above....the His tag and several others. Another peptide-based strategy uses an enzyme to covalently label the ...: 9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion...PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single...protein are available (Cranfill et al., 2016 and FPbase.org). A number of variants remain fluorescent under...

  9. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...expression of many different genes was predictable based solely on the promoters and Shine-Delgarno sequences...Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end or paired-end...segments that can be engineered to bind specific RNA bases. By swapping out these different modular repeat ... the variant PUF segments required to bind any 8 base RNA sequence. The segments are provided in Gateway...

  10. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...Medical School/Brigham and Women’s Hospital (BWH). Based on her experience co-directing Mentor Circle Programs...-Europe professional collaborations, setting up bases and contacts with Spanish laboratories to start ...key to the success of this program. As we build a base of former mentees, we encourage them to get involved...consulting firm focused on new health care models based on integrative and eco-social health strategy, and...their degree. Students filled in a pre-program, baseline self-assessment survey to determine their expectations...expectations of the program and their baseline skills. Mentors were Spanish scientists (MD, PhD and MD/...defining expectations of the program and their baseline skills and a post-program self-assessment, participants...

  13. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ... after I arrived, I was looking through a paper-based, affordable, and portable microscope called the ... known as gas vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial...

  14. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... serine recombinases and tyrosine recombinases, based on sequence similarity and the amino acid residue...the search bars or sort buttons to find plasmids based on your expression system of choice, promoter, or...

  15. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...ends are compatible, meaning that the overhanging base pairs on the vector and insert are complementary... to adjust the amount of DNA used in a ligation based on the length of the DNA to get a proper ratio of...

  16. A Look at Addgene's QC Process

    Type
    Blog Post
    ...in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature” library — with some... the deposited scientist  The backbone elements based on the information provided and vector type  Any...assembly issues that arise due to the short 200–250 base pair reads sequenced on the Illumina MiSeq.  Resolving...

  17. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...have proposed developing new cloning techniques based on these vectors (Shareck et al., 2004). Plasmid...al., 2023). In this post, we’ve grouped plasmids based on the phenotype they offer their host cell, but...Lee, B., & Miguez, C. B. (2004). Cloning Vectors Based on Cryptic Plasmids Isolated from Lactic Acid Bacteria...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... that need them. Adeno-associated virus (AAV) – based delivery techniques may hold the key. In the simplest...effective. Challenges for AAV gene therapy Although AAV-based gene therapies are promising, scientists are working...should be taken into account when developing AAV-based therapies. Furthermore, deeper understanding of ...

  19. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...Francois St-Pierre’s lab developed a new form of FACS-based cell screening, Single-cell Phenotypic Observation...Plasmids and Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair) is a double-strand ...double-strand break repair assay for mammalian cells. It's based on the introduction & resolution of two DSBs in a...eliminate competing mobile genetic elements. Image by Basem Al-Shayeb in the Doudna Lab. Scientists looking...

  20. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ... you. Traditional restriction enzyme and ligase based cloning is a relatively inexpensive way to get the...a try. This method, developed by Invitrogen and based on lambda phage integration sequences, relies on...inserts a breeze. Among them are some of the homology based methods, like Gibson or SLIC, since one can pool...
Showing: 261 - 280 of 710 results