We narrowed to 381 results for: BRE

1. Targeted Mutagenesis with EvolvR

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   Blog Post

   ...tracing cells that do not tolerate double-strand DNA breaks by introducing a unique tag into a genomic site...

2. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

   Type

   Blog Post

   ... it available to everyone, that creates more breakthrough and innovation. With any kind of open platform...

3. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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   Blog Post

   ...These cells, presumably the cell you want, cannot break down X-gal and are white. Cells that don’t contain...

4. Building and Validating Recombinant Antibodies: A Case Study

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   Blog Post

   ... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...offers high efficiency of inducing double strand breaks and more opportunity for homologous recombination...

6. Plasmids 101: Blue-white Screening

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   Blog Post

   ... When expressed, the β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo...

7. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   ... you are interested in and this is a great ice breaker. Of course, real relationships go further and grow...

8. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ...the day cannot be developed through conventional breeding; synthetic biology is required to achieve this...

9. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   Blog Post

   ...(A) Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible. ...

10. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ...schedule might work. If not, perhaps a 3 hour breakfast once a month. All participants must commit to ...

11. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...

12. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    ...transcription instead of introducing a DNA double strand break. Transient introduction of CRISPRi or inducible ...

13. RNA Extraction Without A Kit

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    Blog Post

    ...broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late 1970’s, Chirgwin...

14. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ... different stages of development, ensuring the breadth and versatility of this tool. Find CreLite at Addgene...

15. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...obvious explanation, but more often than not I had to break down exactly what I had done in order to identify...

16. What's New in CRISPR - November 2021

    Type

    Blog Post

    ...transcriptase (RT), which induces two double-strand breaks and deletes the intervening sequence. As with other...

17. dTAG - You're it!

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    Blog Post

    ...not necessary for the proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693....

18. Visualizing Protein Turnover In Situ

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    Blog Post

    ... tags influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific...

19. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ...study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors...

20. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...function of guiding Cas9 to generate a targeted break. These advances in gRNA technology have enhanced...