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We narrowed to 444 results for: CRISPR

Showing: 261 - 280 of 444 results

  1. Addgene: The First Twenty Years

    Type
    Blog Post
    ...And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs....org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online course...

  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...assemble and test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered...etc. MoClo is especially useful for more complex CRISPR experiments (e.g., many guides/parts delivered)...delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, ...

  3. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...morning, John Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this... Institute lab is doing creating well-optimized CRISPR screening technology. Addgene got several shout-outs...Use Addgene's Microbiology Pages Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...

  4. CRISPR Plasmids - Plants

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Plants CRISPR Plasmids: ...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...CRISPR expression plasmids that have been designed for use in plants. CRISPR...: Plants Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  6. Lentiviral Prep Service

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    Collection
    ...Packaging Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes...libraries 92385 Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A Human CRISPR inhibition library...Root 92386 Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set B Human CRISPR inhibition library...genes. For more information on CRISPR plasmids and function, see our CRISPR plasmids resource page . Cas9...Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control...gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes...92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...

  8. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...induced pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations...looking at a bunch of different strategies with CRISPR-Cas9. There are so many different variants and ...functional role of those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse...

  9. Brzezinski Lab CRISPR Collection

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    Collection
    ...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...genomes using exogenous enzymes: Lambda red and CRISPR Developed over the past 20 years, Lambda Red recombineering...used  (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation ... "RNA-guided editing of bacterial genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): ...15109831. Pyne, Michael E., et al. "Coupling the CRISPR/Cas9 system with lambda red recombineering enables...blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn more about Lambda Red recombineering...

  11. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...101 blog series Learn more about CRISPR and genome editing in our CRISPR 101 blog series Resources on Addgene.org... in gene modification tools and methods such as CRISPR have vastly improved scientists’ ability to use...use in the axolotl including plasmids for use in CRISPR and Tol2 transposase induced transgenesis.  Xenopus...Xie, Z., Zhang, C., & Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional...

  13. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...Addgene Blog Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture...; more recently we have also been using Addgene CRISPR vectors to generate cell lines with knockouts of...the 48-well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western...

  14. CRISPR Plasmids - Prime Edit

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    Collection
    ... CRISPR Prime Edit CRISPR Plasmids: Prime Edit Browse CRISPR Plasmids By Function ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime... . Browse, sort, or search the tables below for CRISPR prime editing plasmids. Plasmids are available ...

  16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation...developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish...systems, and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite... Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection Read our molecular biology reference...

  17. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for making...localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab...

  18. Resolute Plasmid Collection

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    Collection
    ...Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled libraries are useful ...Human SLC Activation Library 132561 Superti-Furga CRISPR activation library targeting human SLC proteins...Human SLC Knockout Library 132552 Superti-Furga CRISPR knockout library targeting human SLC proteins. ...Mouse SLC Knockout Library 141436 Superti-Furga CRISPR knockout library targeting mouse SLC proteins. ...Knockout Libraries 213695 221409 221410 Superti-Furga CRISPR knockout library targeting human transmembrane ...

  19. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400 citations in its first...much, you can see the 2013 ‘bump’ related to the CRISPR/Cas papers and Addgene deposits, but papers published... Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (2013): 819-823....

  20. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering...contrast to using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering... et al. "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643...
Showing: 261 - 280 of 444 results