We narrowed to 443 results for: DUR
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TypeBlog Post...delegating for success. Meet regularly before during and after the assignment to discuss goals, expected...
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Plasmids 101: Mammalian Vectors
TypeBlog Post...however, may not be a problem depending on the duration or other parameters of your experiment. Mammalian... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...because they are only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...off-target cleavage should be an important consideration during any experiment. Sensitivity Cas9 sensitivity is... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post... Addgene, we now use next-generation sequencing during our QC process. This way, we can confirm the sequence... -
Viral Vectors 101: Viral Vector Elements
TypeBlog Post... supplied by the production cell line (HEK-293) during production. Fun fact: There are 57 human adenovirus... -
Viral Vectors 101: Biosensors
TypeBlog Post...stimuli and as well as changes in cellular dynamics during so-called housekeeping activities. ... -
Viral Vectors 101: Types of viruses
TypeBlog Post...do not require breakdown of the nuclear envelope during cell division to enter the nucleus as gamma-retroviruses... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...breakdown of the nuclear envelope, which occurs during mitosis. The most well-known retrovirus is HIV.... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...you are studying a protein that may bind a ligand during your experiment, then you’d want to make sure that... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...double-checking details, or just taking a moment to relax. During really stressful days, I would even take advantage... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...motivations, strengths, and learning styles. For example, during my time as a lab scientist, I worked with a diverse... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...’re here for and we’re glad to be able to do so during this critical time. Visit our COVID-19 Plasmids... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...materials in our catalog are very well-characterized. During this time, we’ve also created a variety of educational... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...only increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...unfollow those who do not capture your interest during that week.” You can also find Dr. Knoepfler on ... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post... ligation step determines how ends are resolved during nonhomologous end joining. Nat Commun 5: . https... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post...love to help especially when solving problems. During the conference The conference may start in the... -
Plasmids 101: Yeast Vectors
TypeBlog Post...however, they tend to be unstable and may be lost during budding. Yeast Centromere plasmids (YCp): These...