We narrowed to 650 results for: EPO
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TypeBlog Post...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...
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Using Addgene's New Viral Service for Your Research
TypeBlog Post...money producing virus from select vectors in the repository yourself, you can now order ready-to-use virus...of the most popular lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...webpage for any specific recommendations from the depositing lab. If your vector is large (>10 kb) or has ...4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...robust immune response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog Post...While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...Resources Learn More about Viral Vectors Browse the Repository ... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx... -
Hot Plasmids - January 2023
TypeBlog Post... plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed...ubiquitous promoter drives expression of an mCherry reporter. After using mCherry for FACS sorting of the ... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with all ...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ... -
25 Work From Home Tips for Scientists
TypeBlog Post...career exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for...experiments carefully. Consider submitting a registered report, where peer review is based on the methods, not... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post... fields, including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...Purification Protocol Video rAbs in the Addgene repository... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...ability to infect cells with a high efficiency and deposit a DNA/RNA cargo into their targets makes them attractive...Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...s take a look at a few examples with plasmids deposited at Addgene. PRESTO-TANGO: The ligands that activate...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate...control protein activity Read about RANbodies, reporter nanobody fusions Learn more about using the secondary... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I led. ... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post... information on each cancer clinical sequencing report includes rare variants that haven’t been characterized...MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319. PubMed Central PMCID... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...antibodies expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the... VH and VL sequence. These plasmids have been deposited with Addgene for distribution to the scientific... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...make your mentor want to keep working with you is report back with progress. Tell them how their advice ... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...research. Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule...