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Showing: 261 - 280 of 621 results

  1. How to Lead a Great Meeting

    Type
    Blog Post
    ...everyone gets a chance to talk and less vocal team members still have their voices heard. Here's a template...of the agenda, and assign each one using group members’ initials. If appropriate, email this around after...to help keep you accountable for moving along. Remember, many of the more in-depth discussions can happen...

  2. Lentivirus ddPCR Titration

    Type
    Protocol
    ...of DMEM complete instead of a viral dilution). Remember, this is an additional 10-fold dilution of the...

  3. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...viral envelope, which fuses with the target cell’s membrane, allowing the viral particle access into the cell...inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses can accommodate...of murine leukemia virus DNA depends on mitosis.” EMBO J. 12 (1993) 2099-2108. PubMed PMID: 8491198. PubMed...

  4. Clear and Accessible Writing

    Type
    Blog Post
    ...and improving accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and ... at the Society for Neuroscience conference in November 2023, we hosted our very first professional development...clear writing Let’s use Figure 1 as our example. Remember, our goal is to use words to describe the image...

  6. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...and names, swapped leadership, and grown to 25 members.  What is Sample6? Sample6 is an enrichment-free...technologies, effectively communicating the advantages embodied, and driving the enterprise value of Sample6.  ...Read On! Synthetic Biology & the Importance of Assembly Standards Story of a SynBio Startup: RevBio's ...

  7. Brain Initiative Collection

    Type
    Collection
    ...-GfaABC1D-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000-AAV1...

  9. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... indirect (secondary) methods. In those cases, remember that a secondary should be used when signal amplification...price point, shipping time, or other factors. Just remember that these are secondary considerations to validation...validated using one or more of the proposed pillars.  Remember, validation does not mean that the antibody is...

  10. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... grow. We are hiring again and getting ready to embark on new exciting projects. Right now we don’t .... We are making an impact.  {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist..._playlist=True, viral_sharing=False, embed_button=False, width='1280', height='720', player_id='7039679868...

  11. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...found at Addgene! Figure 1: Strategy for assembling the barcoded gRNA library pool. (Adapted from...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12...your experiments. Figure 2: Strategy for assembling barcoded combinatorial gRNA libraries. (Adapted...

  12. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...While this method seems simple, I found it hard to remember to describe all four parts of a STAR answer once...Writing a rough outline of these stories helped me remember them for the interview.  Prepare a list of questions...then thank them once the interview is complete. Remember, your interviewers took time out of their day ...

  14. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...poster readable While we are talking about font, remember this: bigger is better! Your poster should be ...poster more navigable.  Choose your content wisely Remember that less is more! Posters should not look like...easily read. Similarly, be cognizant of audience members that are color-blind; avoiding red and green is...

  15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...Protein A and B interact, the FP fragments can assemble the full structure of the fluorescent protein,...Split superpositive GFP Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting...behavior of the split FP in your system of interest. Remember that the FP fragments themselves can sometimes...

  16. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic... thawed more than once. Fibronectin is another membrane-interacting protein that can be used to enhance...receptor-independent virus adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. ...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...In cell-free expression systems, proteins are assembled in vitro using purified components of the transcription...Cell-free expression systems are ideal for fast assembly of more than one protein in one reaction. A major...advantage of these systems system is their ability to assemble proteins with labelled or modified amino acids...

  18. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...designing primers, and running analyses. New team members have taken up to a month to design a single 96-...began building CrispyCrunch in July 2018 and in November, we began using it internally. Guiding principles...biopython, bwa, samtools, bowtie, UCSC genome browser, Ensembl, and others. It builds on existing popular web ...

  19. PhD Applications After COVID

    Type
    Blog Post
    ...always a good idea to introduce yourself to a few members of your desired department during the application... Different platforms have different quirks, so remember to practice on the program you’ll be using for... be difficult. However, an online meeting with members of your desired program will allow for you to have...

  20. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...Jorgensen Lab recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR genomic...SapTrap offers scientists a convenient one tube assembly reaction to generate high-throughput targeting...templates. A final ligation reaction correctly assembles the final targeting vector (Fig. 1, Step 4), and...
Showing: 261 - 280 of 621 results