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We narrowed to 801 results for: Eng

Showing: 261 - 280 of 801 results

  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ... that both the peak excitation wavelengths and peak emission wavelength of the two proteins should be ...with shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like...the best protein for a given application is a challenge. To help keep you abreast of the latest fluorescent...

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...components of EvolvR and its potential applications. Challenges of existing mutagenesis methods Several continuous...drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...recombineering and Multiplex Automated Genome Engineering (MAGE) will always be limited by the size of ...investigate non-coding segments of the genome, or engineer microbes to perform tasks. EvolvR could also be...about other CRISPR methods for bacterial genome engineering   Learn more about lambda red recombineering ...

  3. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary...to making cancer precision medicine a reality. Challenges for cancer variant characterization For many ...validation experiments. But, there have been two huge challenges preventing us from testing this hypothesis: ...Accelerator Plasmid Collections To overcome this first challenge, we’ve developed the Broad Institute Target Accelerator...cancer variants To overcome the second major challenge, we developed assays to test large numbers of ...

  4. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...insoluble granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones...editing for psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins...community-centric science, our team has continuously engaged with a variety of stakeholders from industrial ...harmonious progress.     We’re always delighted to engage with potential collaborators and interested audiences...synthetic biology experiment Read our Genome Engineering Guide ...

  5. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...all”. Each team member has unique motivations, strengths, and learning styles. For example, during my time...have led to frustration, micromanagement, and disengagement. By tailoring my leadership approach to each...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...team meeting to highlight their complementary strengths. By encouraging alignment on goals and clarifying...

  6. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...Sample6 still leverages the core engineering and scientific concepts to engineer bacteriophages and use those... As a biomedical engineer and scientist, I like to envision that my research will have a direct impact...himself from the idea of starting a company around engineered bacteriophages. He decided to quit his "safety...his co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...

  7. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...recombineering-based MAGE (Multiplex Automated Genome Engineering) technology. pMA7CR_2.0 expresses lambda Red ...References 1. Maddalo, Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with ..., et al. “Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids.... Sakuma, Tetsushi, et al. “Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector...plasmids from this paper at Addgene. 5. Tsai, Shengdar Q., et al. “Dimeric CRISPR RNA-guided FokI nucleases...tools for efficient germline and somatic genome engineering in Drosophila.” Proceedings of the National Academy...

  8. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering instead ...reduced-in-size options often have reduced binding strength, half-lives, and/or specificity as well. They’... repeat proteins, or DARPins, are genetically engineered small affinity proteins, around 14-15 kDs in ...produced in E. coli, and are both soluble and easily engineered.  Monobodies are ~10kD synthetic binding proteins...

  9. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... more about how you approach new challenges.     Know your strengths! Research experience presents ample...matter of applying that framework to the myriad challenges we encounter. As you spend time in a lab learning...yourself if you missed any important details. Or challenge yourself to think of more creative solutions. ...employer that you are well equipped to work through challenges from day one in your new position. Of course,...

  10. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...elegans Neuronal Gene Expression Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene...characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For more information...information about the aims and approach of CeNGEN, check out their publication.   3’UTRome The 3’UTRs of...popular! References Hammarlund, Marc, et al. "The CeNGEN Project: The Complete Gene Expression Map of an...

  11. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...emphasizing the antibody’s specificity. Strength and accuracy The strength is based on binding specificity and...expression constructs to be conveniently modified and engineered.   Fig. 1: Antibody production methods... developed a criterion that includes accuracy, strength, and reproducibility. We test the antibodies on... negative control also determines the signal’s strength. Exposure time is the amount of time the sample...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...Here's what you'll find in this post: Epigenetic engineering with single-pot assembly Tracking cell-cell interactions...New items from the viral service Epigenetic engineering with single-pot assembly By Eric Perkins Though...epigenetic researchers custom design their own engineering plasmid tools in about a week. Taking advantage...H3K27me3), a linker between these modules (linker length can and should be varied as it can have important...in AAV1!  New ready-to-use AAV prep from Hongkui Zeng's lab are available for dual expression of cytoplasmic-tdTomato...

  13. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... sensor (GEX), where one wavelength changes with Ca while another wavelength is insensitive. Additional...requiring only a simple measurement. However, single wavelength measurements are more susceptible to artifacts...calcium reporter derived from troponin C. It is engineered with a reduced number of calcium binding sites...Cre-dependent expression. These sensors have also been re-engineered to measure changes in calcium specific to the...

  14. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...Most things in biology are not so simple, and engineering complex cellular behaviors requires making complex...output, etc., which can support a larger cellular engineering goal, several of which are described below. Multigene...sometimes many times), and isolated afterwards. To engineer these production pathways, MoClo has been used... an array of distinct goals within metabolic engineering, synthetic product production, and gene editing...  References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular...

  15. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...infrared light to dissipate excess energy at wavelengths of low photosynthetic activity. This still allows...allows photosynthetically active wavelengths to reach zooxanthellae, the coral’s symbiotic dinoflagellates...Forster’s lab used codon optimization programs to engineer 14 chromoprotein sequences into E. coli BioBrick... Wistrand-Yuen, P., … Forster, A. C. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial...bacterial synthetic biology. Journal of Biological Engineering, 12(1). https://doi.org/10.1186/s13036-018-0100...

  16. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...fluorescent signal can be affected by AT-rich DNA and the length of the amplicon and it has a higher rate of false...background noise, and is not affected by AT content or length of the amplicon. As the probes can have several...available, ddPCR may be a better option for more challenging cases.  Price point If budget is a concern...confounding by AT-rich DNA and by variation in amplicon length. Am J Trop Med Hyg. 2008 Dec;79(6):887-92. PMID...Abu Bakar, S., AbuBakar, S., Hollox, E. J., & Boon Peng, H. (2015). A comparison of assays for accurate ...

  17. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell 157.6...not perform functional tests. The newest genome engineer sharing the stage with much-lauded CRISPR-Cas9...cleavage (1). Both the CRISPR-Cas9 and NgAgo genome engineering methods have their own pros and cons (2). The...of a sunny day in Edinburgh.   References 1. Gao, Feng, et al. "DNA-guided genome editing using the Natronobacterium...

  18. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...and offered engaging examples of how understanding genetics is so timely.” Genome engineering, and many ... an authority in this area. What is genome engineering? How does CRISPR work? This section was essentially...computers and the internet before it, genome engineering technology is moving along much faster than it...much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad...

  19. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...and projects. It is crucial to identify these strengths within yourself, and to demonstrate them to the...collaboration and fail to identify it as a personal strength until it is identified by another person. Similar...situations where you can learn more about possible strengths and weaknesses, and view everything as a learning...presents itself even outside of work, consider challenging yourself, take the opportunity, and discover ... strengths...

  20. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...to solve a problem. But how do you intentionally engage in a creative problem-solving process?  You may... needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding ...you can, set aside time to prepare for future challenges. This doesn’t have to be time-consuming or even... keeping your science going throughout these challenging times.  Take a Break! If all else fails, one ... Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your ...
Showing: 261 - 280 of 801 results