We narrowed to 365 results for: Fus
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TypeBlog Post...different applications such as fluorescent protein fusion studies and the generation of mutant cell lines...
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Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...system indicates that it is very tricky to use and fussy to optimize. One reason could be the instability... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...construct is inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post... single point mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these... -
Bacterial Expression Systems
TypeCollection...between the RNAP subunit α fusion protein in pBRα-β flap and the λCI fusion protein in pACλCI-β-flap results...interest are fused to AraC or a dominant negative AraC. Preferential association of AraC-fusion homodimers... expression of an N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease ...and expression of a C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease ...fluorescent proteins Various PIs Fluorescent protein fusion: contains empty vectors with N and C-terminal fluorescent...that can be used to create fluorescent protein fusions with your protein of interest. See an overview ... of interest once its appropriate cDNA has been fused to the MS2 binding sites within this plasmid. pZE31... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...Cas9 expression is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post... bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...inquiry – purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post... Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...directed binding site. These methods include the fusion of an activation domain such as VP16 to dCas9 or... -
CRISPR Plasmids - Mammalian Expression
TypeCollection...CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers...Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine ...Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help visualize... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection... is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this fusion protein...examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy...Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various organelles...organelles or subcellular structures. You should fuse your protein of interest with a fluorescent protein that...Membrane CARMIL2 BH domain tdTomato John Cooper *Fusions to other FPs can be found by searching the complete...Microtubules ensconsin mCherry William Bement *Fusions to other FPs can be found by searching the complete...Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions to other FPs can be found by searching the complete... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...e.g. GFP) can be used in many ways. Often a FP is fused to a protein of interest to elucidate the subcellular... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...economic importance as harmful weeds. The parasitic fusion is such that the two plants share RNA, proteins... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...Specifically, SHH is a morphogen, a chemical signal that diffuses down a concentration gradient exerting a different... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... Our Stem Cell Collection Transcription Factor Fusions for Enhanced Reprogramming Find plasmids from the... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...also small enough to deliver with AAV. This system fuses catalytically dead dCas13b to the catalytic domain... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...Editing for Programmable A to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post.... They don’t simply rely on chance (or random diffusion) to make this happen. There are several different...