We narrowed to 372 results for: Fus

1. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...system indicates that it is very tricky to use and fussy to optimize. One reason could be the instability...

2. PITChing MMEJ as an Alternative Route for Gene Editing

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   Blog Post

   ...construct is inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific...

3. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   ... single point mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these...

4. Antibodies 101: Producing Recombinant Antibodies

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   ... bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin...

5. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ...inquiry – purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and...

6. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   Blog Post

   ... Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...

7. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   ...sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining...

8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...directed binding site. These methods include the fusion of an activation domain such as VP16 to dCas9 or...

9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...Cas9 expression is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic...

10. Hot Plasmids Spring 2024

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    Blog Post

    ...Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined...

11. Bioinformatics at Addgene

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    ... containerization, but haven’t tried it or are confused by the concept, don’t worry–you are not alone!...

12. Viral Vectors 101: Pseudotyping

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    Blog Post

    ...envelope protein causes cells that it infects to fuse together and form syncytias that cause the cells...

13. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...also small enough to deliver with AAV. This system fuses catalytically dead dCas13b to the catalytic domain...

14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post

    ...Editing for Programmable A to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific...

15. Plasmids 101: Plasmid Incompatibility

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    Blog Post

    .... They don’t simply rely on chance (or random diffusion) to make this happen.  There are several different...

16. An Inside Look into Addgene's Dev Team

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    Blog Post

    ...might be calling it an aliquot. This can lead to confusion as to what the code is supposed to be doing. When...

17. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...e.g. GFP) can be used in many ways. Often a FP is fused to a protein of interest to elucidate the subcellular...

18. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    Blog Post

    ...economic importance as harmful weeds. The parasitic fusion is such that the two plants share RNA, proteins...

19. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post

    ...Specifically, SHH is a morphogen, a chemical signal that diffuses down a concentration gradient exerting a different...

20. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

    ... Our Stem Cell Collection Transcription Factor Fusions for Enhanced Reprogramming Find plasmids from the...