...Addgene, who will be taking over the distribution to expand the network of scientists working on this even more...throw a therapeutic monkeywrench into the virus’ hijacking of human cells. Even better, more than half the...the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one FedEx package at a time Since...interest, because it means more scientists will be working on this problem more quickly. Figure 2: Getting...headache receiving constant email requests and tracking their fulfillment manually in a shared spreadsheet...interested in facilitating collaborative science, and making it accessible to all people, not just scientists...

...Review dropped a bombshell report. A scientist working in China was using CRISPR/Cas9 in an attempt to...can happen. We just have to look to the United Kingdom’s Nuffield Council. While the Nuffield Council has...tasked two separate non-governmental groups with seeking “public views on emerging IVF techniques designed...addition to thorough ethical considerations, in making sure that the research has been adequately proven...experiment on humans, and they are not remembered kindly. However, despite many headlines questioning if...otherwise, the scientific process does seem to be working. I would argue that one of the takeaways from He...whom are named Charlotte, all of whom are still working on theirpenmanship. Additional resources on the...

...distanced work more creative. An example could be making lab meetings more interactive and creating space...interaction and brainstorming. This could be achieved by asking each presenter to come up with a scientific question...: “Science is increasingly done in large teams, making it more likely that papers will be written by several...taste of that casual kitchen chatter, ultimately sparking new ideas. Social no-science-y meetings Set up...probably lost during these weird times of shift working and social distancing. The take home message is...are endless. Let’s adapt and embrace new ways of working, reconnect as human beings and colleagues, eventually...confidence to launch new and diverse careers by taking part in career panels, and volunteering for scientific...

...projects range from hacking probiotics to filming educational lab videos, while some are looking to develop new...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate in the... gene drive paper was very popular. People kept asking if we were teaching classes at the lab, so in December...That’s right, we have non-traditional scientists working in our laboratory! Don’t worry though, we make ...transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse range of...

...for tagging experiments and it’s already looking like it’s making a big difference. We can now look at many... an adjunct faculty member) have together been working toward this goal for years as members of the ENCyclopedia...actually use this for tagging any protein in all kinds of different assays, not just transcription factors...variations on the plasmids we’ve developed. We’re working on that, but the system is so flexible. Other investigators...Fragment from Integrated DNA technologies. Generally speaking, anyone using CRISPR/Cas9 in the lab already should...Thank you to Professors Mendenhall and Myers for speaking with us! Find the Plasmids: Plasmids for protein...

...interested in the same phosphate of your favorite kinase is a good start. Check out Joanne's Reddit AMA ...general intro until you know if the person you are talking to is really in your direct area.  Review the conference...see when your connections post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn...colleague. Recommended Reading & More: Scientist Networking: What is an Informational Interview? Crash Course...Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships for Success" webinar... improving your networking and soft skills ... scientist networking...

...continued working for Palladian Partners remotely, but started looking for a new job because “it kinda sucked...social media manager. “I usually start out my days looking through our Twitter feeds and dealing with social...difficult pieces: “It’s probably the best.” Besides working through the tough assignments, the skills that ...include: “Listening. Evaluating evidence. Critical thinking. Being able to learn things quickly. Story telling...government, so it was a really good environment to be looking for a job.” Thanks to Susan for not only being ...

...Coaching can be an excellent tactic for scientists making a career shift or who wish to improve their current... responsibility seriously. As I make my rounds speaking at research institutions and at scientific conferences...take some research.  It helps to spend some time thinking about what your goals are for the coaching relationship...out of academia (because so many scientists are making this transition). Another coaching group is Still...Humphrey from Jobs on Toast is based in the United Kingdom and scientists there would be lucky to work with...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. I...Entropia Consulting works remotely, will travel for speaking engagements and has many areas of specialty from...

...pretty good about sticking to that. If you email us at noon and then call back at 1PM asking why you haven...Tweeting and texting, actually writing an e-mail or making a phone call is a big commitment when you’re busy...doctoral dissertations. Streamline the question asking process Having thought through the above, don’t...soon as possible!” and I always begin my responses asking for more specifics. We can help you faster if we...how long it will take to get an answer, consider asking to avoid unrealistic expectations. And finally... Eric J. Perkins...

...with different kinetic parameters. There are three GCaMP types based on their kinetics: GCaMPf (fast) ...bones and teeth. It is an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator...sensor to use for your studies, you can start by asking ‘What is the biological phenomena I want to observe... a sensor that is tuned to your parameters.   Kinetics How fast is the process you would like to monitor...jGCaMP7 and jGCaMP8 are also available with improved kinetic and sensitivity parameters. Sensitivity and Localization...to variance in sensor expression if you aren’t working with a stably integrated biosensor. Ratiometric...

...ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand is also important for ...delayed and takes on the order of minutes. Studies looking at immediate effects of specific neuron firing ...blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies is whether or ... are viable for translation to human patients. Picking receptors The receptor you choose is largely dependent... you to directly manipulate ion channels. When working with PSAM receptors, your impact on the cell is...Liss B, Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic and pharmacodynamic actions of clozapine-N-...

...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion...light is more phototoxic to cells, most scientists working on in vivo imaging models perfer the PA-RFPs over...from a dark state to a bright fluorescent state (“kindling”), and from a bright state to a dark non-fluorescent...fluorescence usually means more photons are being emitted, making it easier to capture a clear image above background...for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred for studying...PMC2901231. 3.Verkhusha, Vladislav V., and Alexander Sorkin. "Conversion of the monomeric red fluorescent protein...Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast Saccharomyces cerevisiae...

...governing policies are developed primarily by wishful thinking. The March for Science is about fighting scientific...postdoctoral researcher at Harvard Medical School working on human artificial chromosomes. Montreal, QC, ...scientists, we work tirelessly in the lab where the groundbreaking research happens, sometimes forgetting how ... I was scared. I worried that, because we were speaking out against legislators and because the march ...AggleDetector on Twitter) is a graduate student at Johns Hopkins University, where he is trying turn a few exoplanets...it takes to complete your resume, without ever thinking that research scientist would be a career that...NIH budget were proposed. I spent a lot of time asking why. As scientists we spend years being taught ...

...the intensity of fluorescence signal and enables tracking of single molecules within living cells without...roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell cycle. Good...different chromosomes. The advantage of having this kind of system that works in live cells is that one can...used with your own reporters to decipher their trafficking routes. This collection is now available at Addgene...indicators should possess fast voltage sensing kinetics and have no spectral overlap with the optogenetic...and an endoplasmic reticulum export motif and trafficking sequence for improved targeting to the plasma...CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator, QuasAr...

...hardware sold by the manufacturer. Blocking solutions Your blocking options come in two categories: protein-based...make when designing a western blot.  Psst! Looking for a western blot protocol? Check out Addgene'...antibodies are relatively cheap and easy to source, making it easy to switch from one readout method to another...re running native (non-reduced) proteins or are looking to preserve specific protein modifications, we ...single-percentage gel, which is cheap and useful when you’re looking at proteins all roughly the same size, or a slightly...information. Many antibodies will have a recommended blocking buffer and a recommended concentration. That makes...greater dynamic range than chemiluminescence enzymes, making it easier to accurately visualize proteins of wildly...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...undergrads back in grad school. Both were hard-working scientists-in-training who got the job done, but...left him/her with small tasks and s/he was always asking for more work (this is a big hint that you are ...on what you should be doing : writing a grant, thinking about the next project, reaching out to other ...really helpful. “What is the best/worst part of working with X?”  is going to get you what you really want...grateful for the feedback, but at least you can start making changes for the best. Positive feedback is equally...Scientists Series What Makes a Good Mentor? Tips for Working in Industry and Success in Management Resources...

...the conformational dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage of the self-labeling...and more photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques...deposited an array of constructs for tagging the kinesins KIF5A and KIF5B as well as backbones for generating... will be useful not only for future studies of kinesins but for many other proteins of interest too! Find....A.S., Craggs, T.D., & Twelvetrees A.E. (2024) Kinesin-1 is highly flexible and adopts an open conformation...voltage imaging. A) ASAP5 selected for its improved kinetics and responsivity in screening ASAP3 variants. ...different illumination regimes with fast activation kinetics and high responsivity near the resting membrane...

...researchers learn many transferable skills while working in the lab and by offering advice on areas where... jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists negotiate...approached it as a creative problem-solving exercise? Asking questions like the below can help you understand...you are in the middle of a frustrating situation. Asking for opinions from a neutral third party can be ...offer conflicts with a pre-planned vacation I’m looking forward to. How can I negotiate my starting date... a tenured professor or signing contracts when working for a business development team, negotiation is...strengthen your relationships with others who know that working with you will benefit them too. Ed's note: Salary...

...structural biology, Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution...model. Until then, you can only wonder what I am talking about. How do researchers use physical models of... over and over in their hands. They then start talking to themselves as they compare the features displayed...magical teaching moments. The models function as thinking tools – eliciting questions like “what does this...investigated in their labs. Soon after we began working with educators in our Center for BioMolecular Modeling...complementary DNA strand. And this is why we love working with researchers on a model. The little knowledge...Jennifer Doudna’s new book A Crack in Creation. Making models more useful through flexibility It is common...

...of His-tagged human kinases in bactera. Listen to Our Segment on The Human Kinase Domain Constructs Kit...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ...CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized point mutations. Listen to Our...Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's Lab -...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...