We narrowed to 768 results for: LIS;

... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...containing that enzyme, and embedded in the References lists on every page you can find direct links to plasmids...content and much, much more.     Fig. 2 – List of resources and properties for a Cas entry (SpyCas9a...      CRISPRpedia IGI’s digital resources in English/Spanish  ...

...Tools to Try  Checklists Found in surgical suites, air fields, and military bases, checklists are the ultimate...accurately. Thankfully, you should already have a checklist for everything you do in the form of a protocol...protocol a few times, you no longer need the full checklist. Great! But don’t just stop using it cold turkey...

...take a course through the school of journalism on science journalism.” She also interned two days a week...Susan  finds important for her work include: “Listening. Evaluating evidence. Critical thinking. Being...Addgene Blog Volunteer to write for the Addgene blog Listen to Our Podcast Episode on Science Communication...

... vice versa, let’s take a look at cancer cell metabolism. Cancer cells promote oxidative stress leading...of cells affect tumor progression, enzymes and metabolism reprogramming aren’t the whole story. It’s essential...Portugal. She is particularly interested in science journalism and writing about biomedical issues. Follow her...260. PubMed PMID: 16807804. Hanahan, Douglas, and Lisa M. Coussens. "Accessories to the crime: functions...

... for more offerings. The viral vector team is publishing research, hosting conferences, and are expanding...documentaries in 3 languages (Japanese, German, and English) and even on 60 Minutes. We are blessed with a ...often focus on the excitement of what we are accomplishing and what more we might do. I am grateful every...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...

...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...gene expression (see www.nursa.org for a curated list of coregulators). Coregulators can function as coactivators... regulatory regime (such as genes involved in metabolism, growth or cytokine action). Primary or core ...the NURSA website will feature direct links to listings of available plasmids for our curated NRs and ...

...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...misconduct. Past ethical breaches such as the Tuskegee syphilis experiment, the case of Henrietta Lacks, the Havasupai...between these communities and the biomedical establishment. This, along with concerns about the use of ...obtaining my PhD and working in digital academic publishing for a year, I’ve since returned to pgEd as a ...

...dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids...designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little more complicated. These...the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the dsRNA from bacteria and..., is available from Addgene. If you use these published protocols and want to share pictures, feedback...

...team from China’s Ribobio, the illustrious speaker list attracted a host of sponsors based all over the ...one take dried seahorse for?), but there were no English speakers around. Opening talks – Genomics discoveries...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...resistant bacteria. See the picture to the left for his list of the bacterial species that “ESKAPE” antibiotic...

...enrichment via enzyme-linked immunosorbent assay (ELISA) The enriched sybody pools from the phage display...the protein of interest. The Seeger lab used the ELISA technique described in their protocol because it...starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the ...detected using a colorimetric reaction. After the ELISA step, the narrowed-down pool (usually 10-30) of ...

...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still... antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...

... is a list of poster titles or abstracts available before the conference, comb through that list and note... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...been an area of intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection... transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated... to perform, often show low efficiency, while biolistic bombardment, which can have high efficiency, requires...

...’ button a new content-aware suggestion dropdown list will let you jump directly to the type of information...to the plasmid page at the bottom of the dropdown list: The search bar will suggest direct links to other... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...the ‘X’ next to any filter to clear it. Here’s a list of available filters to refine your search results...

... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the...purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western blots...tags and fusion proteins as examples. The tables list more common tags than are described in the post,...Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification is provided...

...fluorophores are constantly being engineered. Table 1 lists a few common fluorescent proteins and their mutations...mutations relative to wildtype GFP. Although not listed here, many permutations within each color also exist...plasmids as a means to many functional ends. We’ve listed our favorites below, but many other uses currently...Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing the best...

...reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized microtubule...transport, and cell division, but given the long list of roles played by microtubules, Opto-katanin will...understand their roles in forming the cilia that help establish left-right organization of the embryo (Aljiboury...the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically controlled phospholipase...

... contest is now closed. Stay tuned to see the finalists and vote for your favorite! Our mascot who has...neutral name. The Addgenies will choose up to four finalists and we’ll ask you to vote for your favorite name...We hope you submit your best ideas because the finalists will receive some newly designed Addgene swag....

...Mammalian Cells Note: This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong...However, proper control experiments are needed to establish the dynamic range of your assay. These experiments...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure... developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental...

...Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist, Rebecca. They will be working...is rounded out with our science communication specialist (you'll know me by my Tweeting — er, posting!...