We narrowed to 480 results for: Mag;

...schools. I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of...optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance ...

...cellular repair mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette...

...cargo with the excision-only piggyBac™ transposase. Image courtesy of Transposagen.   How does piggyBac... selection cassette by excision-only piggyBac™. Image courtesy of Transposagen.   How does the piggyBac...

... and obtain a knockout mouse in about 3 months. Image courtesy of Wenning Qin. Single-stranded DNA...electroporation is performed immediately after injection. Image from Ohtsuka et al., 2018. Used with permission ...

...aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly.... These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for...

...5, 6, 8, and 9 in two different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used...both oligodendrocytes (Olig2) and neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under ...

... is twice the length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to ...multimer identification.   Fig. 2: Gel image of Plasmid 61564: pBAMD1-2 (~4.7 kb) digested with...

...paper. Pretty much like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas...chemiluminescence, is detected on x-ray film or with specific imaging devices. In most cases, the primary antibody, which...

...and nearest sample. To minimize the risk of DNA damage, it is best to limit the UV exposure of the DNA...DNA. Therefore, it is a bad idea to use a gel imager to take a picture of the gel before cutting out the...

...: PMC146043. 9. Nilsson, Stefan V., and Göran Magnusson. "Sealing of gaps in duplex DNA by T4 DNA ligase... using a capillary tube, probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical...

...and viability. If mycoplasma has made permanent damage to the cell culture it is irreparable. Some cells... Biotechnology.       References Degeling MH, Maguire CA, Bovenberg MSS, Tannous BA (2012) Sensitive ...

...scientist was like. The word “scientist” conjured up images of men like Albert Einstein and Bill Nye in their...postdocs I’ve met have done things that I never even imagined before I set foot in the lab. They’re also more...

...plate was scanned for V5 tagging using an Operetta imaging system. Dewari et al, eLife 2018;7:e35069 DOI: ...clonal lines must be cautiously analyzed for any damage (indels) to the promoter region of the non-HDR ...

...set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant... deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...

...solution and then rinse it in water before you can image the gel. Pour the agarose into a gel tray with the...expose for as little time as possible to minimize damage to the DNA. Note: When using UV light, protect ...

...dimers. (c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma...allows, you can fit in a quick game of Connect Four! Image created in BioRender.   Passive Reference Dyes...

...Device Roller Spatula Platform shaker Cold room Gel imager -80 °C freezer Reagents 1X PBS Lysis buffer e.g...Cover the membrane with clear plastic and use a gel imager with chemiluminescence detection or a dark room...

...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...

...temperature, as prolonged storage in the cold room may damage it. Using a pipettor, transfer the top, aqueous...

...that responds to general signals of infection or damage. Insertional mutagenesis Random insertion of DNA...