We narrowed to 365 results for: Pol;

1. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are having difficulties...

2. Viral Vectors 101: An Introduction to AAV

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   ...vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually...

3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...spectroscopic methods (based on fluorescence polarization) can be used to detect homodimerization. However...

4. CRISPR 101: Cas9 vs. The Other Cas(s)

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   ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

5. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...used to create any program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the...

6. Career Coaching for Scientists: Why and Where Do I Find One?

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   ...PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats,...

7. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   Blog Post

   ... P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong ...

8. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   Blog Post

   ...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

9. Open Resources and Plasmid Tools For Studying C. elegans

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   ... C. elegans 3’UTRs structures, alternative polyadenylation for all protein coding mRNAs, and annotations...

10. CRISPR in the Clinic

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    Blog Post

    ...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

11. A Look at Addgene's QC Process

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    ... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...

12. Design Tips for Prime Editing

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    ... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

13. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier...

14. Hot Plasmids and Viral Preps - January 2021

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    Blog Post

    ... CRISPR human genome-wide library deposited by Leopold Parts. This library delivers 2 randomly paired ...

15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

    ...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...

16. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    Blog Post

    ...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...

17. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...

18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

19. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    ..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

20. Magnetic Control of Proteins: More than a Dream

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    Blog Post

    ...References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C....