We narrowed to 613 results for: SET
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TypeBlog Post... as to how the shared reagents should be used. Setting clear expectations from the beginning makes the...
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Viral Vectors 101: Transductions
TypeBlog Post...easily achieved with alternative methods. Ready, set, transduce! Making virus can sound daunting, but ... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...long email. If you have more to say, email again or set up a meeting. If you really got along well, suggest... -
Plasmids 101: TOPO Cloning
TypeBlog Post...polymerase using your favorite PCR protocol. 2. Set Up TOPO Cloning Reaction: Mix together the PCR product...topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening... -
Another Pathway into Cells: iTOP
TypeBlog Post...must be evaluated on a case-by-case basis. While setting up their CPP system to study protein transduction... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...with a different dCas9 binding module, alternative sets of transcriptional programs can be layered with ... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...then the chance is that you have handled large data sets and are familiar with statistical analysis. If you... If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers usually don't... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post... and detailed protocols on topics such as how to set up your digests and pouring/running a DNA gel. ...glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a visual reference... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post... to create a fully controllable and reproducible set up,” Bernach explains. Developing an artificial leaf... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...they developed CRISPRainbow (Ma et al., 2016), a set of gRNA scaffolds that contain two hairpin sequences..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...mutation rate by only ~2-fold over nCas9 levels. Two sets of modifications were also made to PolI3M to increase... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...and plasmids to researchers in resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid... -
DAP arrays for multiplex gene editing
TypeBlog Post... base editing and prime editing. How do you then set up an MBE system that contains a double-win for both... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...template for PCR amplification with a degenerate primer set to amplify IgG variable light (VL) and variable heavy... -
Hot Plasmids - January 2023
TypeBlog Post... Yingxiao Wang lab. The crux of the system is a set of heat-inducible lentiviral CAR constructs, the ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository... -
How to Lead a Great Meeting
TypeBlog Post...for team meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...and the CARE guidelines for case reports are two sets of guidelines frequently used by editors. Providing... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...series will cover 2 of the 7 mentoring questions I set out to answer. First, I will describe some of the... -
Brain Initiative Collection
TypeCollection...research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository.... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...Buy American, Hire American" Executive Order which sets broad policy intentions directing federal agencies...ensure that each candidate meets the requirements set forth by USCIS before engaging with clients. To learn...