...structures demonstrating “the structure is strikingly similar to the design model at atomic resolution” (Kuhlman... design is possible. Although Top7 was designed simply to be stable and novel, the principles that created...

...in the biological sciences and techniques that simplify and expedite research. We recognize, however, ... blog! To sign up to write for the Addgene blog simply email us. We can’t guarantee that all those who...

...that within that article you’ll find new plasmids. Simply click on the badge to be taken directly to the ...an account on PubMed. Once you have an account, simply click on your account name in the top right corner...

...application. The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate...can work through it at your own pace. To submit, simply click "Contribute Data."    Figure 6: Contributing...

...ladders are not next to empty wells. Most people will simply load an extra ladder or technical replicate (ie... leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget to start it...blocking time! Fill the time with smaller tasks, or simply enjoy some well-deserved down time while waiting...

...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...

...advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase reporters...swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited spectral...

...DePace’s yearly planning meetings revolve around two simple worksheets, each of which is filled out separately...Extracurricular Activities for a Strong Science Career Path 7 Simple Strategies to Resolve Conflict With Difficult Supervisors...

... it to fruition. The concept, though relatively simple, changed the course of scientific research. What...of requesting and sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey...

...cerevisiae and S. pombe. The protocol is surprisingly simple, requiring only a PCR product containing the modifying...ensure that all desired tags have been integrated. Simply design your amplification primers with the desired...

...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased...AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is a very common...

...this simple act directly associates you with a network of researchers who may be working on similar projects...

...this over 30 minutes, which was fun to watch. A simpler 1 protein - 1 RNA system Drs. Dounda and Charpentier...into one RNA to direct cleavage the result was a simpler 1 protein - 1 RNA system.  Dr. Charpentier advocated...

...designed in the same vector backbone with very similar restriction enzymes. You can essentially cut and...quickly. Researchers should keep in mind that a very simple sequential or double restriction enzyme digest ...

...analyzing deposits with many plasmids that contain similar features and it allows you to more quickly identify...size of the plasmid and the insert. By using the “Simulate agarose gel” option with the desktop version of...

...spent collecting it will have been worthless. But simply writing down your data doesn’t guarantee a publication...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...

...such as ChR2 and NpHR, can even be expressed simultaneously and still be individually activated with different... By looking at groups of neurons that elicited similar tail movements, they were able to narrow down the...

...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At...Suitability for in vivo experiments, due to their simple structure    sdAbs in research These advantages...

...even blogged about this here and here); however, simply put, I moved into this position because I want ...will find useful and interesting than that will simply get people ordering plasmids. The need to keep ...

...e.g. titer, injection site, animal strain used). Similar to the goodwill of Addgene plasmid depositors, ...this resource for the community. There are a few simple steps you can take to input your data for sharing...