We narrowed to 729 results for: STO

...well received, and then when the team started brainstorming ideas for the next workshop, they realized that...to practice and teach reproducibility An early milestone for the group was the “reproducibility for everyone... Schwessinger for helpful conversations on the history of the group, lessons learned, and to Sonali Roy...

...Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters enable constant...2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage...Synthetic Biology: A Conversation with MIT’s Christopher Voigt Resources on Addgene.org See the synthetic...

...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August... studies, the edited embryos proceeded to the blastocyst stage at similar rates to untreated controls....researchers were able to examine each individual blastomere to determine its genotype. However, this detailed...

...indicated below and pool-clone them into the pAWp28 storage vector (Figure 1): Forward oligo: 5’- CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC...bp barcode, respectively. Once cloned into the storage vector, the pooled, barcoded gRNA library is now...backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage vector to the pAWp12 lentiviral vector should retain...

...part of the recognition sequence, they can be customized to direct assembly of DNA fragments. When designed...utilize the BsaI and BsmBI Type IIS sites such that custom TAL arrays could be constructed quickly and efficiently...Nov 5. PubMed. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...

...experiences. Good coaches will have loads of success stories and plenty of people to vouch for them. Trust... of scientists here) I know many coaches in the Boston area. Sarah Cardozo Duncan is a proven coach who...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

...your target protein in each well. The reaction is stopped by the addition of hydrochloric acid (for more ...(TMB). The addition of hydrochloric acid, which stops the reaction, turns the solution yellow. The absorbance...serial dilution of your experimental sample, and/or stopping the HRP-TMB reaction more quickly. Just make sure...

...born before 1985, you might remember going to the store and buying CDs when you wanted to hear a piece of...technology makes it easy for scientists to share, remix, store, and track these essential components of the scientific...useful in perpetuity.  Second, plasmids are easy to store and share, making recombinant antibodies the most...

...plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand breaks...very long ncRNAs is impractical. Instead, tags or stop elements can be inserted to disrupt the transcription... adjacent genes are complex and not yet fully understood, so it is important to carefully select the target...

...When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with biotin. ...but labeling ceases when these two bait proteins stop interacting. This feature is particularly useful...

... In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication programs... and money to a full-time graduate course, UWE Bristol offers an intensive 4-day Masterclass in science...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

...science, dating back several hundred years, with historical ties to psychology and philosophy (the mind-body...in a certain type of behavior (for a review see Stoica et al., 2013). Vector delivery may also be achieved...service for their behavioral studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer...

...so many scientists who were potential Addgene requestors and depositors. China is in one of the top 10...good job of seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab ...can view public circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq...

...Women’s Hospital/Harvard Medical School and then Boston University (institutions where I’ve worked as a...became interim Chair automatically as it was an understood expectation of any Vice-Chair. Following an official...Juliet Moncaster is an Assistant Professor at the Boston University School of Medicine and is the former...

...spectral flow and can be thought of as a series of histograms flipped onto its side and compressed into a heat...for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody binding...: Secondary AntibodiesAntibodies 101: Buffers, Storage, and Conjugates Resources on Addgene.org Addgene's...

...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

...methods. First, its modular design allows for customization with strain-specific promoters, antibiotic resistance...species where its modular nature allows for the customization of guides, promoters, selection markers, and...(2005): 443. PubMed PMID: 15908923. Johnson, Christopher M., and Alan D. Grossman. "Integrative and conjugative...

... inner cell mass (ICM) of a 5-6 day old human blastocyst [6]. They are isolated mostly from abnormal and...PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective of stem cell research. Best Pract...Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med, 2004. 350(13): p. 1353-6. PubMed...

...approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months before... completed in one long day, or the virus can be stored at 4 °C and buffer exchanged the next day. Refer... comments below! Special thanks for Dr. Dan Stone and Harshana de Silva Feelixge at Fred Hutchinson...

...course, you will want to make sure that your final stocks only contain rAAV. To minimize contamination, use...preparation.  rAAVs  Now your AAV virions are ready to store or use. They are quite stable and will withstand...Londono, L., Arjona, K., Parianos, M., Truong, V., Rostonics, E., Hernandez, A., Boye, S. L., Boye, S. E., ...