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We narrowed to 712 results for: STO

Showing: 261 - 280 of 712 results

  1. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...experiences. Good coaches will have loads of success stories and plenty of people to vouch for them. Trust... of scientists here) I know many coaches in the Boston area. Sarah Cardozo Duncan is a proven coach who...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

  4. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...your target protein in each well. The reaction is stopped by the addition of hydrochloric acid (for more ...(TMB). The addition of hydrochloric acid, which stops the reaction, turns the solution yellow. The absorbance...serial dilution of your experimental sample, and/or stopping the HRP-TMB reaction more quickly. Just make sure...

  6. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand breaks...very long ncRNAs is impractical. Instead, tags or stop elements can be inserted to disrupt the transcription... adjacent genes are complex and not yet fully understood, so it is important to carefully select the target...

  7. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ... In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication programs... and money to a full-time graduate course, UWE Bristol offers an intensive 4-day Masterclass in science...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

  8. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...so many scientists who were potential Addgene requestors and depositors. China is in one of the top 10...good job of seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab ...can view public circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq...

  9. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...Women’s Hospital/Harvard Medical School and then Boston University (institutions where I’ve worked as a...became interim Chair automatically as it was an understood expectation of any Vice-Chair. Following an official...Juliet Moncaster is an Assistant Professor at the Boston University School of Medicine and is the former...

  10. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...methods. First, its modular design allows for customization with strain-specific promoters, antibiotic resistance...species where its modular nature allows for the customization of guides, promoters, selection markers, and...(2005): 443. PubMed PMID: 15908923. Johnson, Christopher M., and Alan D. Grossman. "Integrative and conjugative...

  12. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months before... completed in one long day, or the virus can be stored at 4 °C and buffer exchanged the next day. Refer... comments below! Special thanks for Dr. Dan Stone and Harshana de Silva Feelixge at Fred Hutchinson...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

  14. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...course, you will want to make sure that your final stocks only contain rAAV. To minimize contamination, use...preparation.  rAAVs  Now your AAV virions are ready to store or use. They are quite stable and will withstand...Londono, L., Arjona, K., Parianos, M., Truong, V., Rostonics, E., Hernandez, A., Boye, S. L., Boye, S. E., ...

  15. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...spectral flow and can be thought of as a series of histograms flipped onto its side and compressed into a heat...for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody binding...: Secondary AntibodiesAntibodies 101: Buffers, Storage, and Conjugates Resources on Addgene.org Addgene's...

  16. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...biology also falls within this section. The GFP story has its own Franklin in Doug Prasher, who originally...describes the detection of gravitational waves--a story I was much less familiar with as a biologist--as... feel slightly dated by next week.   A stepping stone When I discussed The State of Science with the five...

  17. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...for a scientist. I think that establishing this history helped demonstrate that a) I was a real person ...which demonstrated to the students that scientific history was very much in the making. One of the exciting... in an exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes...

  18. Tips for arabidopsis transformation

    Type
    Blog Post
    ...using and whether you are inoculating from a frozen stock or a colony from an LB plate. In my hands, a 5 mL...culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once: Dip your plants...University. She studies cell fate maintenance in the stomatal lineage and has made countless transgenic plants...

  19. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ....io beta platform for sharing, annotating, and storing life science protocols. Since we began sharing ...management and you want the works: organizing reagent storage and inventory, managing orders, tracking students... scientists workflow in mind and can offer a one-stop solution to your lab's organization and management...

  20. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...different codons that encode 20 amino acids and three stop codons, meaning that the same amino acid can be ...codons that encodes the 20 amino acids and three stop codons. (B) Example of codon usage among different... References Athey J, Alexaki A, Osipova E, Rostovtsev A, Santana-Quintero LV, Katneni U, Simonyan V...
Showing: 261 - 280 of 712 results