We narrowed to 421 results for: SUA
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TypeBlog Post...samples. To minimize these issues, researchers usually include a separate normalization step where individual...
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New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post..., et al. “An optimized fluorescent probe for visualizing glutamate neurotransmission.” Nature Methods ... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post... site is limited, and we all know that science usually doesn’t follow our timelines! Prepare a research... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...different ways. This leads me to my last tip: It’s usually not worth connecting with people you don’t like... -
Cultivating Community Science at BosLab
TypeBlog Post...is not required. Our members are skilled with audiovisual media, software engineering, business consulting... -
How to Lead a Great Meeting
TypeBlog Post...employees. 15-30 minutes weekly or monthly will usually suffice to keep both parties on the same page. ... -
Management for Scientists: Delegating is Key
TypeBlog Post...’t delegate tasks assigned to you and you must usually take on all tasks that involve confidential information... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...concentration required for efficient selection. This is usually achieved by performing a "kill curve" (basically... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment ... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...resistance gene linked to an in-frame H2B-GFP. This visual read out allows for easy screening of different... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...assay paper turning it purple, allowing for easy visual detection of the presence of the norovirus. While... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...conditions. The properties of fluorescent proteins are usually only examined under a few conditions since it is... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue. References... -
Antibodies 101: ChIP
TypeBlog Post...selective nature of the cutting. While sonication is usually the preferred method, enzymatic digestion can be... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...page to a few key reminders on a sticky note Visual Planning Plate maps, pre-labeling tubes, and even... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post... you need a non-canonical amino acid, which is usually added to the media for uptake by the cells. Second... -
How Addgene Creates Content
TypeBlog Post...not shown. However, for our blog posts, we usually make figures in BioRender or use appropriately ... -
Plasmids 101: Screens vs. Selections
TypeBlog Post...screening experiment. Thus, a limited panel is usually selected. The method you choose to assess traits... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...plasmids to elucidate the role of 14 of the 16 non-visual heterotrimeric G proteins that can be activated... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...which grow optimally in moderate temperatures (usually defined between a fairly nippy 20°C to a decidedly...