We narrowed to 424 results for: SUA

1. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   Blog Post

   ...dL5** was tagged with mCerulean3 (mCer3) for visualization and expressed from a heart-specific promoter...

2. Pushing the Limits of DNA Assembly

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   Blog Post

   ...cost effective and eliminates the annealing step usually used to create a double-stranded oligo. Generating...

3. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

   ...samples. To minimize these issues, researchers usually include a separate normalization step where individual...

4. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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   Blog Post

   ...FlipGFP-based caspase reporter can be used to visualize apoptosis of enterocytes in the midgut of Drosophila...

5. The Importance of High Titer for AAV Transductions

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   Blog Post

   ...than others. High titers are often necessary to visualize expression of fluorescent proteins, for example...

6. Cultivating Community Science at BosLab

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   Blog Post

   ...is not required. Our members are skilled with audiovisual media, software engineering, business consulting...

7. What Good Citizenship Can Do for Reproducibility in Science

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   Blog Post

   ... struggles with reproducibility at some point. Usually it comes up when a researcher decides on a new ...

8. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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   Blog Post

   ..., et al. “An optimized fluorescent probe for visualizing glutamate neurotransmission.” Nature Methods ...

9. Networking Follow-up: I Met a Person. Now What?

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   Blog Post

   ...different ways. This leads me to my last tip: It’s usually not worth connecting with people you don’t like...

10. How to Lead a Great Meeting

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    Blog Post

    ...employees. 15-30 minutes weekly or monthly will usually suffice to keep both parties on the same page. ...

11. Management for Scientists: Delegating is Key

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    Blog Post

    ...’t delegate tasks assigned to you and you must usually take on all tasks that involve confidential information...

12. Plasmids 101: Mammalian Vectors

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    Blog Post

    ...concentration required for efficient selection. This is usually achieved by performing a "kill curve" (basically...

13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post

    ...is high enough to negate the need for selection usually required in recombineering. REPLACR-mutagenesis...

14. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

    ...domain of one ITR. These small deletions are not usually an issue as they are thought to be self-repaired...

15. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post

    ... you need a non-canonical amino acid, which is usually added to the media for uptake by the cells. Second...

16. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post

    ...resistance gene linked to an in-frame H2B-GFP. This visual read out allows for easy screening of different...

17. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ...which grow optimally in moderate temperatures (usually defined between a fairly nippy 20°C to a decidedly...

18. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    Blog Post

    ...conditions. The properties of fluorescent proteins are usually only examined under a few conditions since it is...

19. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment ...

20. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    Blog Post

    ...assay paper turning it purple, allowing for easy visual detection of the presence of the norovirus. While...