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Showing: 261 - 280 of 582 results
  1. How Addgene Creates Content

    Type
    Blog Post
    ...It should be no surprise then that a lot of our subject matter comes straight from you, whether you realize...second review from someone less familiar with the subject for clarity. Our content covers a lot of very ...
  2. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the 2’ position...advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient and less toxic to...
  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...CLOVER Center at CalTech? If so, you may have submitted a question that didn’t get answered live - but...Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...
  4. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside... genes that allow for the use of a particular substrate in defined medium, without which the cells cannot...
  5. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-ligase IV); together with some...Aprataxin, Tdp2) that function to make ends better substrates for ligation. Although we do not describe these...
  6. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...experiments. These background proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications...
  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...
  8. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4. This can be important...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...
  9. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...-wrmScarlet labeling proteins with different subcellular locations. Image from Goudeau et al., 2020. ...
  10. CRISPR in the Clinic

    Type
    Blog Post
    ...experiencing sustained and substantial relief from both disorders. As of publication, submissions to the UK, EU, ...
  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...want to consider using an aliquot that has been subjected to a freeze/thaw cycle for titering as this will...
Showing: 261 - 280 of 582 results