We narrowed to 579 results for: SUB

...CLOVER Center at CalTech? If so, you may have submitted a question that didn’t get answered live - but...Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...

...freeze-thaw-freeze cycle before, or otherwise been subject to mishandling?  Is the experiment you’re planning...the same assay. This is one of the most complex subjects in antibody-based protocols - but thankfully this...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... Weiss’s group used these reporters to define a subset of active miRNA’s unique to HEK293FT and HeLa cell...

...promoters commonly used in plant research. Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit ... releases heat shock factor 1 (HSF-1), which subsequently binds to heat shock elements in the promoter...

...documents the target substrate (DNA/RNA and strandedness) along with its action on the substrate (nick, bind, ...

...β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside... genes that allow for the use of a particular substrate in defined medium, without which the cells cannot...

...for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-ligase IV); together with some...Aprataxin, Tdp2) that function to make ends better substrates for ligation. Although we do not describe these...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...Assembly of plasmids using the Cyanogate Kit and subsequent transformation into cyanobacteria. Image from...

...secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4. This can be important...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...photo-reversible protein-protein interactions can control subcellular localization processes in vivo. They deposited...

...caution, since the difference between studies is substantial (e.g, the poor performance of mRuby2 is detected...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...analysis of the kinetics of chromatin regulation and subsequent mathematical modeling. Its reversibility allows...

...helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...

...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...experiments. These background proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications...

...other references to scientific literature to substantiate the facts upon which it builds. This means you...need to be on a network with an institutional subscription), archived on PubMed Central, or in preprint...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...-wrmScarlet labeling proteins with different subcellular locations. Image from Goudeau et al., 2020. ...

... which proteins assemble into functional multi-subunit macromolecular complexes, but a serendipitous discovery...sites in T4 lysozyme by deficiency-creating substitutions." Journal of molecular biology 277.2 (1998):...

...experiencing sustained and substantial relief from both disorders. As of publication, submissions to the UK, EU, ...

...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...want to consider using an aliquot that has been subjected to a freeze/thaw cycle for titering as this will...

...Essentially this reporter construct consists of one substrate (the histone-derived peptide) which can localise...together and causes an increase in the FRET signal. Subsequent demethylation separates the fluorescent units...