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Showing: 261 - 280 of 578 results

  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside... genes that allow for the use of a particular substrate in defined medium, without which the cells cannot...

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-ligase IV); together with some...Aprataxin, Tdp2) that function to make ends better substrates for ligation. Although we do not describe these...

  3. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...MFI makes it easier to identify and study these subtle shifts. Additionally, you can gate off the histograms...the presence/absence of your marker but there are subtle shifts in expression, using a histogram plot and...

  4. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR; Long terminal repeats; U3...bigger than ∼3 kb are packaged less efficiently. Subcomponents: U3; Unique 3'; region at the 3' end of viral...

  6. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...CLOVER Center at CalTech? If so, you may have submitted a question that didn’t get answered live - but...Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...

  7. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...freeze-thaw-freeze cycle before, or otherwise been subject to mishandling?  Is the experiment you’re planning...the same assay. This is one of the most complex subjects in antibody-based protocols - but thankfully this...

  10. How Addgene Creates Content

    Type
    Blog Post
    ...It should be no surprise then that a lot of our subject matter comes straight from you, whether you realize...second review from someone less familiar with the subject for clarity. Our content covers a lot of very ...

  12. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters commonly used in plant research. Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit ... releases heat shock factor 1 (HSF-1), which subsequently binds to heat shock elements in the promoter...

  15. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...experiments. These background proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications...

  17. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4. This can be important...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...

  20. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the 2’ position...advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient and less toxic to...
Showing: 261 - 280 of 578 results