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...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview...

...populations of interest. Many of the calcium sensors described are also already packaged into viral vectors and...

...ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details...

... use a modified version of the Python program described by Joung et al. 2017, which we hope to make available...

...cloning, the Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly...

...Between SpRY and several of the other Cas variants described, nearly all genomic landscapes should be editable...

...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ...

...for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of...

...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...

...organizations, I asked audience members to stand and describe unique nonprofit models that differed from the...

... assemblies per plate using outgrowth volumes described in Figure 3, with calculated yields from entire...

...sense, models can be both useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students...

...(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila...

...Maria were encouraged by previous reports that described magnetic responses of cryptochrome proteins or...

...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...

...chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored protein from the jellyfish...

...proteasome for degradation. Like several other tags described here, the dTAG uses a small molecule, a tag, and...

...that prevents RNA polymerase from efficiently transcribing the gene. This results in gene silencing, with...

...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in...

...complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex...