Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 561 results for: Spr;

Showing: 261 - 280 of 561 results

  1. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...and improved. The CRISPR posts written by our guest bloggers, the experts in the CRISPR/Cas field, have ...past year. A quick glance shows that interest in CRISPR continues to grow as the genome editing technique...Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest Blogger) ...Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered! By Caroline LaManna Plasmids...

  2. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...piggyBac™ Transposon Catch up on Everything CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ... to the development of site-specific nucleases (CRISPR/Cas9, TALENs, and ZFNs). However, several challenges...piggyBac™ transposon system can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations...interest, along with a site-specific nuclease (such as CRISPR/Cas9) to create a double-stranded DNA break. After...technologies including Zinc Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized ...

  4. Hot Plasmids - February 2022

    Type
    Blog Post
    ... Penn State Protein Ladder Engineered miniature CRISPR-Cas system for mammalian genome regulation and ...gene perturbation by: Gabrielle Clouse While many CRISPR-based genetic screening methods have primarily ...10.1038/s41598-021-96051-x Engineered miniature CRISPR-Cas system for mammalian genome regulation and ...and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion proteins, such as Cas9 and...engineering applications. CasMINI, a new miniature CRISPR-Cas system for editing and gene activation, is ...

  5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog series Resources... Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier this month...Addgene.org Find base editors for your research Read our CRISPR guide Find plasmids for your research ...

  6. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics and Editing...Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does this...

  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of choice after...can then be used both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts... Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed...

  8. Design Tips for Prime Editing

    Type
    Blog Post
    ...Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by ...editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer binding site ...Prime Editing: Adding Precision and Flexibility to CRISPR Editing PRIDICT: Predicting Efficiencies of Prime...

  9. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... to. When it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred...1990s and quickly rose to popularity. Compared to CRISPR, RNAi methods are often quicker and the repositories...vast and difficult to beat in terms of size. Like CRISPR, there is a variety of small RNA types to choose..., it was the go-to choice for many years before CRISPR burst on to the scene. shRNAs reliably knock down...in Plant Biology: New Tools for an Old Favorite CRISPR 101: Targeting RNA with Cas13a (C2c2) Plasmids ...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...genomes using exogenous enzymes: Lambda red and CRISPR Developed over the past 20 years, Lambda Red recombineering...used  (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation ... "RNA-guided editing of bacterial genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): ...15109831. Pyne, Michael E., et al. "Coupling the CRISPR/Cas9 system with lambda red recombineering enables...blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn more about Lambda Red recombineering...

  11. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...(Mouse IgG2a) Goat anti-mouse IgG2a CASPR anti-CASPR mAb K65/35 (Mouse IgG1) Goat anti-mouse...Neocortex labeled with anti-pan-Nav (magenta), anti-CASPR (green), and anti-Kv2.1 (cyan). (B) Cerebellum labeled...Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex Immunofluorescence on Fresh...Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935...

  12. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...our collection. CRISPR/Cas9 - At Addgene, 2013 will be known as the "Year of the CRISPR." The newest genome...many different purposes that we had to create a CRISPR page to keep up with all the new plasmids. So far... far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,000 labs around the world. ...

  13. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...applying temporary tattoos on the spot with our trusty spray bottles! We also raffled off one of our highly sought-after...morning, John Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this... Institute lab is doing creating well-optimized CRISPR screening technology. Addgene got several shout-outs...Use Addgene's Microbiology Pages Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...

  14. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...Service, we’re distributing lentivirus (with many CRISPR tools included among the preps that are currently...lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral... activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like to knockout... in experiments other than just those involving CRISPR and so we also have lentivirus for delivering control...

  15. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...Resources at Addgene.org Check out our CRISPR resources and CRISPR for plants Bacterial Expression Systems...PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic engineering ...more information on bacterial engineering using CRISPR on our blog). Her research should provide us with...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    ...And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs....org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online course...

  18. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...assemble and test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered...etc. MoClo is especially useful for more complex CRISPR experiments (e.g., many guides/parts delivered)...delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, ...

  19. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...important role in helping to monitor and slow this spread until vaccine programs can be put in place to provide... people globally live in areas conducive to the spread of the virus by the Aedes mosquito (Messina et ...done as quickly as we could. From the outset, we spread out the risk for the project by initially designing... RNA amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic...
Showing: 261 - 280 of 561 results