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  1. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...the secondary nanobody toolbox Are you ready to start using nanobodies for your own research? Great! Pleiner...Görlich, D., Lee, C., Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies...

  2. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...curriculum. It’s through the PTA that I recently started coordinating the annual Math & Science Night, a...scientist. I gave a timeline of my science life, starting back to Mr. Kelley, my 7th grade biology teacher...

  3. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...specialized equipment and/or expertise from the start, or if you can begin simply and build up to more...potential of your preferred platform before you start can save a lot of trouble in the long run.  Selecting...

  5. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...primer pair. Ct values vary based on the amount of starting material in the PCR because the sample must fall..., which is a factor in AAV titration since the starting template is the purified viral vector solution...

  6. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... Again, attending one of these fairs is a great start, but you need to do more than just show up. Do your... particular employer in mind, you may consider starting with indeed.com or monster.com, where you’ll find...

  7. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... we hope that these tips might help to get you started. Once you have established a well working protocol...Leibniz Universität Hannover (Germany), before starting his work in the Lee-Parsons Lab.     References...

  8. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...may be preferentially charged during amino acid starvation thus resulting in higher production of amino ...charging of tRNA isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID...

  9. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...depend on the host cell’s initiation proteins to start their own replication. In general, these plasmids...binding of the low-affinity versions is required to start the replication process. When enough copies of ATP-DnaA...

  10. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post
    ...SHERLOCK to detect SARS-CoV-2 RNA. The test is started using RNA purified from patient samples and can...applications. The company Mammoth Biosciences has been started based on DETECTR technology with the mission to...

  11. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...is our process for working on a new feature. We start with a story that describes the feature and what...closely with each other and with other teams. We start with writing demo details, step-by-step instructions...

  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...homology to the crRNA or presence of a PFS. This is in stark contrast to Cas9, which requires that each DNA target... we know about its structure and function? For starters, Cas13a can be used to bind and cleave target ...

  14. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...screening experiments as well). Therefore, if you’ve started down the CRISPR path, you’ve probably got a gene... them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing in mice, they...

  15. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...means exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic... gene Although this list is a great place to start, the tables above do not delve into the tissue or...

  17. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... a mutation introduced by CRISPR. The sequence started clean and then, about 100 bps into the run, it ...CRISPR for mouse genome engineering From the very start, generating mouse models using CRISPR is easier ...

  19. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... annually advertise for Group Leader positions starting in August, the year before the position would ...PFs are in committed relationships and/or have started a family. Those people are your support network...

  20. Antibodies 101: Validation

    Type
    Blog Post
    ... is all it will bind to - particularly when you start using complex samples such as whole cell lysate ...be aware of any possible alternative translation start sites or alternative splicing that could lead to...
Showing: 261 - 280 of 562 results