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  1. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post
    ...of common E. coli strains. Increase the volume Starting with a larger culture volume can improve plasmid...serve to increase cell density and plasmid yield. Start with a single, fresh colony Sub-culturing directly...
  2. Anatomy of a Plasmid Page at Addgene

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    Blog Post
    ...in the example above that the bread crumbs trail starts in the browse page, continues through the depositing...the plasmid in your experiments: Backbone: The starting vector that the depositing lab modified to create...
  3. Summer SciComm Series: Modes of Communication

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    Blog Post
    ...specialized equipment and/or expertise from the start, or if you can begin simply and build up to more...potential of your preferred platform before you start can save a lot of trouble in the long run.  Selecting...
  4. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post
    ...the secondary nanobody toolbox Are you ready to start using nanobodies for your own research? Great! Pleiner...Görlich, D., Lee, C., Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies...
  5. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post
    ...curriculum. It’s through the PTA that I recently started coordinating the annual Math & Science Night, a...scientist. I gave a timeline of my science life, starting back to Mr. Kelley, my 7th grade biology teacher...
  6. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post
    ...SHERLOCK to detect SARS-CoV-2 RNA. The test is started using RNA purified from patient samples and can...applications. The company Mammoth Biosciences has been started based on DETECTR technology with the mission to...
  7. An Inside Look into Addgene's Dev Team

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    Blog Post
    ...is our process for working on a new feature. We start with a story that describes the feature and what...closely with each other and with other teams. We start with writing demo details, step-by-step instructions...
  8. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ...primer pair. Ct values vary based on the amount of starting material in the PCR because the sample must fall..., which is a factor in AAV titration since the starting template is the purified viral vector solution...
  9. Transferable Skills Guide: Career Planning Resources

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    Blog Post
    ... Again, attending one of these fairs is a great start, but you need to do more than just show up. Do your... particular employer in mind, you may consider starting with indeed.com or monster.com, where you’ll find...
  10. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ... we hope that these tips might help to get you started. Once you have established a well working protocol...Leibniz Universität Hannover (Germany), before starting his work in the Lee-Parsons Lab.     References...
  11. Plasmids 101: Stringent Regulation of Replication

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    Blog Post
    ...depend on the host cell’s initiation proteins to start their own replication. In general, these plasmids...binding of the low-affinity versions is required to start the replication process. When enough copies of ATP-DnaA...
  12. Plasmids 101: Codon usage bias

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    Blog Post
    ...may be preferentially charged during amino acid starvation thus resulting in higher production of amino ...charging of tRNA isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID...
  13. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ...homology to the crRNA or presence of a PFS. This is in stark contrast to Cas9, which requires that each DNA target... we know about its structure and function? For starters, Cas13a can be used to bind and cleave target ...
  14. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...screening experiments as well). Therefore, if you’ve started down the CRISPR path, you’ve probably got a gene... them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing in mice, they...
  15. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...means exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic... gene Although this list is a great place to start, the tables above do not delve into the tissue or...
  16. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post
    ... a mutation introduced by CRISPR. The sequence started clean and then, about 100 bps into the run, it ...CRISPR for mouse genome engineering From the very start, generating mouse models using CRISPR is easier ...
Showing: 261 - 280 of 570 results