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  1. Of Myc and Men

    Type
    Blog Post
    ...and monoclonal antibodies. However, it was (and still is) infeasible to generate highly specific antibodies...EQKLISEEDL) - larger than the substance P peptide, but still quite small. However, unlike the substance P tag...

  2. Transferable Skills: Negotiation

    Type
    Blog Post
    ... related to the actual data collection task but still be beneficial to both.  Coming up with creative ...with the company so they know I’m excited while still being able to take my vacation? I want to learn ...

  3. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can.... When complexed with a truncated gRNA, Cas9 is still able to bind DNA but is no longer competent to cut...

  4. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...(Figure 4). When recombination occurs, the gene still functions normally because one of its exons has ...are located in intronic regions, this gene will still be expressed. You'd first screen for this specific...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...predominantly fluoresce one color but slowly mature until they predominantly fluoresce a second color. This...multiple systems Terskikh et al. showed that their versatile timer could be used in vitro, in mammalian cells...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Restriction digest screening for ITR deletion is still necessary. To save time and money, I like to miniprep...on the Addgene blog Find out what makes AAV a versatile tool for gene expression in mammals Use the PEI...

  9. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...impressive, full-color images of proteins. But they are still flat. To truly appreciate the 3D structure of these... will understand the power of a physical model. Until then, you can only wonder what I am talking about...

  10. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...expression of the gene it follows. For increased versatility, it would be advantageous to adapt PITCh to insert...-independent knock-ins, but this possibility is still being explored. More broadly, Sakuma et al.’s use...

  13. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...its first steps! However, retroviral vectors can still direct the translation of proteins because they ...and transduction by vectors (which we sometimes still call “infection” as a shorthand, although it is ...

  15. DNA Purification Without a Kit

    Type
    Blog Post
    ...regeneration, trace amounts of DNA (3 femtograms/ul) could still be eluted from the column, as detected by qPCR, ...break-up with your DNA purification kit, you can still reduce the number of spin columns you need to complete...

  17. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...and well-documented history of racial bias, which still impacts and influences current research. Understanding...five-team response to address this issue, but it still points to a far larger and more complex problem ...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...Additionally, AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression...and gene therapy AAVs for genome editing AAV: A versatile tool for gene expression in mammals Resources...
Showing: 261 - 280 of 607 results