We narrowed to 398 results for: aga

1. Summer SciComm Series: Cognitive Load

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   ...you!)  Amount of information and level of detail Again, this is an obvious one on the surface, but it’s...

2. Antibodies 101: Polyclonal Antibodies

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   ...full-length protein that you want to generate antibodies against, or a unique peptide sequence derived from this...

3. Addgene: The First Twenty Years

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   ...in r/molecularbiology.   Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production...

4. To Each HIS Own

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   ...from a complex mix using affinity chromatography against an amino acid sequence, or tag, on the protein....

5. All in a Twist: dsRNA

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   ...found (typically RNA that the RNAi is designed against), RISC can repress translation and/or degrade target...

6. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...localization of the hM4Di, we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold...

7. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ...of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the Addgene Blog Learn How...

8. Addgene's Tips for Plasmid Quality Control

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   ...enable you to quickly BLAST your sequencing result against the expected sequence for quick verification, which...

9. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ...tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells with GFP, at a ...

10. New Tool for Lineage Tracing: The ClonTracer Library

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    ...the parent cell population using the library, propagated it, separated it into roughly identical replicate...

11. Working with Nuclear Receptors

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    ...acquisition, storage, and utilization - and on self-propagation by reproduction. Both physiological processes...

12. Adenoviral Vector Production and Troubleshooting

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    ...ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent adenovirus...

13. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...and selectable markers that allow students to propagate the plasmids in either E. coli (ori and ampR) ...

14. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    ...with an antibody. This can artificially select against PPIs that aren't stable outside the native cell...

15. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...multiple genes to generate a cellular phenotype. Again ABE8.20-m was the winner. It could simultaneously...

16. Open Resources and Plasmid Tools For Studying C. elegans

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    ...the 22nd International C. elegans meeting, I was again reminded of the extent that C. elegans researchers...

17. Plasmids 101: The Wide World of Natural Plasmids

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    ...., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock,...

18. Hot Plasmids February 2024

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    ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

19. Viral Vectors 101: Chemogenetics

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    ...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...

20. Antibodies 101: Stripping and Reprobing Western Blots

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    ...from your membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample...