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...called “Teaching Your Research.” This course has participants take their research from the bench to the... level course based on their research interests. “So for me, I researched G-proteins at the time. This...program takes what you know about it [your area of research], and teaches you how to design a course, how ...off: “I spent about 6 months just editing and searching for a job and I stumbled upon eScience by luck...happenstance. It was on Indeed or one of the job search sites. It's  a rare trek these days to go in cold...

... this position because I want to help make the research enterprise work better and because I really like...back on. While doing and presenting undergraduate research at BU, I realized that I’m energized by talking...difficulties behind great discoveries by tackling a research project and getting a PhD myself. To continue ...graduate student group, Science in the News. While research experience is key to my current position, I think...Kentucky where I spent a couple of days talking to researchers about their work, picking up plasmid deposits...ways Addgene can improve in its mission to make research easier. I’m hoping my story demonstrates that ...mine, that help support academia but the skills researchers gain doing PhDs (i.e. technical writing, teaching...

...capable of at the same time. Barcoding libraries Just as physical barcodes help librarians track individual...individual books, genetic barcodes allow researchers to identify specific cells — as well as their descendants...sequencing the unique DNA barcodes. Figure 1: Schematic of barcoding with a pooled library. Created...from the Samantha Morris lab use a combinatorial barcoding approach to observe clonal dynamics and cell lineage...

...promote reproducibility, Addgene will have unique Research Resource Identifiers (RRIDs) for each antibody...connects colleagues and is invested in improving research infrastructure, from our educational materials...funded by a generous grant from the NIH’s Brain Research Through Advancing Innovative Neurotechnologies...continue to add more antibodies, for neuroscience research and other disciplines, over time. The resource...Resources As part of our commitment to supporting your research, Addgene is creating an array of educational materials... that you will find useful antibodies for your research. Please consider sharing your experiences with...Reproducibility: Standardize antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a...

...fans of all forms of research sharing and with Open Data Day 2020 coming up on March 7th, what better way...resource. Instead of painstakingly searching for experimental details in research articles, scientists can use...experiments using a specific AAV tool. They can search the database by certain features like serotype,...the dedicated community of data-contributing researchers who use AAV tools. This word cloud (using...open data resource and accelerating reproducible research. Share your data on AAV data hub! Related resources...

...scientists have been coming to the Addgene website in search of plasmids. Now, scientists are beginning to see...classification This inquiry started when Neil Thompson, a research scientist at MIT’s Computer Science and Artificial... MIT labs in the Synthetic Biology Engineering Research Consortium. That is how Aditya Kunjapur, a MIT... gene sequences as natural or synthetic, the researchers looked at intrinsic properties (GC content or...85% correlates with synthetic sequences. The researchers then used their classifier to identify synthetic...lab-of-origin of engineered DNA This isn’t the only time researchers have used Addgene as a large data set. Alec A...Addgene.org Check out Addgene's synthetic biology page Search for a plasmid Find Addgene's most popular plasmids...

...year. While we love academic research, we hope we can help all researchers achieve happiness in their careers... on a diverse array of topics pertinent to the research community, two eBooks (Management for Scientists... science and the plasmids, technologies, and researchers behind them. As part of our thanks to you for...and CRISPR 101 series. These posts aim to give researchers the background they need to get started using... bring about the next revolution in biological research. We’re incredibly happy to see that one of our...deletions, and translocations. It also provides researchers with many different ways to control gene expression...

...localization. HiBiT-tagged targets in translational research Researchers can incorporate HiBiT with a wide range ...with knock-in models, ectopic models provide researchers with a means to quickly iterate, work across ...use of HiBiT provides a means to quickly answer research questions before creating knock-ins.  ... Cracking the code of 'undruggable' targets Researchers can design drugs to bind to well-defined ligand-binding...CRISPR knock-in HiBiT-tagged EGFR cells allowed researchers to track endogenous activity of EGFR, including...advances detection of endogenous proteins As researchers continue to improve more physiologically and ...the heterogeneity and spread of hepatocellular carcinoma in human tissue and in vitro. Oncology Reports...

...represent different research areas, target readers, methodologies, or qualities of research our adjustment ...influence the direction of scientific research. View her research on her SSRN Author page: https://ssrn.com... Management. His research focuses on a range of topics including biotech and research tools, computing...Biochemistry & Molecular Biology, and Biochemical Research Methods. We also pulled the big interdisciplinary...

...he helps researchers in a variety of fields develop RNAi screens to answer their own research questions...guide and promote Addgene in its mission to make research easier. Addgene is excited to announce that we...ended... it was actually something bordering on research, rather than 'here’s the protocol, get a result...later realizing that he wanted to continue doing research, Doench went on to apply to graduate school at...incredible place.” Doench has spent most of his research career working on miRNAs and RNAi. He first heard...specificity in the miRNA regulatory process. This early research is still relevant to his work at the Broad Institute...

...wondering “What is this PI looking for in a new researcher?,” “How do I convince the PI to choose me?” We...about awesome science. Important factors in the search for a lab Studies show that job satisfaction (especially...graduate student or postdoc. Although the chosen research field is important, the end goal during this time...tasks like a scientist. Of course, the specific research field should be interesting; however, this should...biotech, this is one case where the topic of your research can make your transition easier – choose a lab...Posts in this Blog Series: Advice for Choosing a Research Project 9 Tips to Achieve Success in Academia... search for a lab...

...mission is to Accelerate research and discovery by improving access to useful research materials and information...Can Use the Viral Service in Your Research Using Virus in Your Research - A Primer for Beginners Resources...lab from MIT and Alla Karpova’s lab from Janelia Research Campus have provided valuable insight into AAV...focus on assisting scientists and accelerating research and discovery, while also pushing us to challenge...Visit Our Viral Vector Pages Find Virus for Your Research ...

...selecting which particular AAVs to use for your research. Below I’ll provide some tips that will help you...think about how to get started with AAVs. Since my research is rooted in the CNS, my focus will be mostly ...tolerated, then axonal transport is fine and a researcher should aim to inject a larger amount of vector...kB [9]).     4. The Future What new types of research may be enabled by the use of AAVs? 1) Combined...potential problem in many experiments, a group of researchers has identified this feature of AAVs as a new ... to study neural control. Surprisingly, little research has compared and contrasted optogenetic and chemogenetic... the most appropriate vehicle for this type of research, as they are safe to use in vivo. 3) The use ...

...remarkable technological advances have taken researchers to the threshold of a revolution in modern neuroscience... channelrhodopsins, the first two found in the Archaea, the third in green algae (Oesterhelt and Stoeckenius...In order to express opsins within the brain, researchers inject genetically modified viruses encoding ... functional topography of the neural circuit architecture of the brain regions associated with motivated...7030. PubMed PMID: 18614669. Belzung, Catherine, Marc Turiault, and Guy Griebel. "Optogenetics to study... in pigmentation." Biochemical and biophysical research communications 78.1 (1977): 237-243. PubMed PMID... Chris, and Kimberly R. Thompson. "Overview on research and clinical applications of optogenetics." Current...

...Gary McDowell, executive director of Future of Research. On December 1st 2016, many postdocs working more...reversing these decisions entirely. At Future of Research, we had been collecting data and documenting the...blogpost and more recently in a paper published in F1000Research on November 2016. Due to the nature of the postdoc...was granted nationwide. Again, we at Future of Research believe that, while the mandate has been removed...what their institution is doing from Future of Research over social media, not from their institution)... currently the Executive Director of Future of Research. He is interested in advocating for and with junior... him on Twitter @BiophysicalFrog and Future of Research @FORsymp.   Additional Resources on the Addgene...

...materials, Sonali Roy, a postdoctoral researcher at the Noble Research Institute, co-instructed the ASPB ...organized a panel to discuss reproducibility issues in research. But he realized that it wasn’t enough to discuss... that running interactive workshops to train researchers on tools and best practices could be an actionable... practices being adopted by a wider group of researchers. Generating material to empower others to practice... the materials in one place and better serve researchers at all levels of the scientific community. Lessons...interactive workshops that train and provide researchers with tools and best practices could be an actionable...

...by guest blogger Fredrik Wermeling, leader of a research group at the Centrum for Molecular Medicine (CMM... different gRNAs. It has been suggested that researchers should use ~400X more cells than gRNAs in a screen...are addressing a well-defined, hypothesis driven research question. Examples of custom CRISPR screens Differentially...analyses like RNAseq are getting lower and lower, the research community is generating a massive amount of descriptive...such data. These targeted screens can allow a researcher to test which of the differentially expressed...embedded. These were kindly donated by six different research constellations: Doench/Root (2), Zhang (3), Wu...screen! Final note I am very grateful to the researchers letting us use their reference libraries thereby...

...than cell cultures do, and tired researchers are accident-prone researchers. Note that safety policies will...have become a regular part of basic biological research as well as clinical therapy. These biological ...in non-target cells, including yourself as the researcher.   Figure 1: Separation of retroviral ... delivered, that is a higher risk cargo for a researcher to come into contact with than GFP. Similarly...need to be done in a biosafety cabinet. Many researchers choose to wear eye protection when they are working...likely to want to cut a few corners. For many researchers, viruses might be the most dangerous things they...

... involved in developing tools and solutions to research problems. This movement calls itself the Open ...and is part of a bigger movement in a variety of research sectors to provide open source technologies and...and spaces where interested parties can do research. This interest manifested itself in the creation of...collaboratively to solve, we want to focus our research on worm locomotion, but our worms are constantly...automated microscope  to keep our worms and our research goals in focus. Developing these skills relies...submit our new strains back to the CGC for other researchers to use. Although we haven’t yet made our final...University of Victoria. After a decade at Microsoft Research working on speech recognition, he returned to ...

...show you how Drosophila can be used to push your research in new and exciting directions. Drosophila are...the fourth and Y chromosomes are miniscule, so researchers generally do not manipulate them. Essentially...accessible resource out there to help keep your research moving. Below, you’ll find some great introductory... resources. As you dive deeper into Drosophila research, you’ll find more resources that may be more tailored...look up information about your fly gene using the search box in the upper right hand corner. They have video...Drosophila reagents are generously shared among the research community. They maintain many fly stocks that ...stocks are available at Bloomington, but you can search for various fly reagents on the Bloomington website...