...efficiency! Ensure that no arcing is taking place during electroporation. Arcing would manifest as a loud...single sample. They are often used for screening, barcoding, or other high throughput multiplexed experiments...

...see these vectors, visit Addgene’s website and “search for “pLKO”“. Back to Top B. Designing shRNA Oligos...example, the Whitehead Institute for Biomedical Research hosts an siRNA Selection Program that can be accessed...Starting at 25nt downstream of the start codon (ATG), search for 21nt sequences that match the pattern AA(N ...N 19 ). If no suitable match is found, search for NAR(N 17 )YNN, where N is any nucleotide, R is a purine...

...-Bsa4 as an example of LIC experimental design. Search Addgene's collection of empty LIC cloning vectors...transformation, following a standard protocol . Search Addgene's collection of empty LIC cloning vectors...

...adeno-associated virus (AAV) preps for use in your research.... as an intravenous isomolar contrast agent. In research, iodixanol is used as an isomolar density gradient...

.... From a single copy of DNA (the template), a researcher can create thousands of identical copies using...synthesizes and ships them as a lyophilized powder. The researcher then needs to reconstitute their primers in liquid...

... is mis-called? Open the trace file and use the search feature in the program to locate the incorrect ...

...antibiotic resistance gene on a plasmids allows researchers to easily isolate bacteria containing that plasmid...

...Welch to discuss her career path, differences in research culture between the US and Australia, and advice...

...Introduction Microscopes are emblematic of biological research and are found in many different types of laboratories...

...while the population expands. At this time, some researchers reduce the concentration of the antibiotic in...

...antibiotic resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid...

...reverse-complement using existing software (a quick internet search will lead you to here and many others). If we ...

...transgene could be lost through recombination. Some researchers choose to maintain cells in selective medium ...

...mM Sodium Citrate, pH 7.0 0.5% (wt/vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol®...