...efficiency! Ensure that no arcing is taking place during electroporation. Arcing would manifest as a loud...single sample. They are often used for screening, barcoding, or other high throughput multiplexed experiments...

...Plasmid Collections KLF Plasmids Plasmids for KLF Research KLF Depositing Labs Juan Belmonte Linzhao Cheng... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...

...Collections Plasmids for Optogenetics Research Plasmids for Optogenetics Research You may also like... Optogenetics...membrane channels and pores derived from algae and archaebacteria, that can be opened and closed in response ...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps that...Guide . Interested in more news about optogenetics research? Find related posts on Addgene's blog. Microbial...Microbial Opsin Plasmids Browse, sort, or search the table below for the optogenetics plasmids for neuronal...neuronal excitation and inhibition. For example, search for "ChR mCherry", "Cre inhibition", "Boyden 2014", ...to top Optical Switch Plasmids Browse, sort, or search the table below for plasmids that use light to ...

... be useful for neurodegeneration research.... Neurodegeneration Research Collection Welcome to Addgene's ...Addgene's Neurodegeneration Research Landing Page! Here you’ll find information and plasmid collections that may... may help with your neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools iPSC Differentiation...studies (GWAS) or other large scale studies. Researchers are now investigating the role that these additional...additional genes may play in Alzheimer's disease. Research Tools Neurodegeneration Plasmid Collection A collection...AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent expression of diphtheria...

... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You May Also ... TDGF1 teratocarcinoma-derived growth factor 1 CR, CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived...Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional...immune response. Browse Plasmids for Immunology Research Genes have been grouped into tables based on the...CD32B, FCG2, FCGR2, IGFR2 FOS v-fos FBJ murine osteosarcoma viral oncogene homolog AP-1, C-FOS GSK3B glycogen...synthase kinase 3 beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1...oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog C-K-RAS, K-RAS2A, K-RAS2B...

...Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research Reprogramming Plasmids Differentiation...plasmids that have been used in Stem Cell research. You can search or sort this table based on plasmid name...Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells are proliferative, unspecialized...have been studied for over 30 years. All of this research led to Kazutoshi Takahashi and Shinya Yamanaka...helping readers choose a strategy suited to their research. Once you know which method you would like to ...create iPSCs or another cell type. You can also search the iPSC Neurodegenerative Disease Initiative (...browse Addgene’s entire collection of Stem Cell Research Plasmids . For more information on stem cells,...

... Special Collections Cancer Research Cancer Research Plasmids...cancer research, organized by signaling pathway. Special...plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR mTOR is a protein kinase...Wnt Coming soon! Kits and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection ..., focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types...oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier Lab RAS Pathway Clone...gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from...

... CRISPR Calarco Lab CRISPR Plasmids Calarco Lab CRISPR Plasmids Available...regarding the protocol please contact Dr. John Calarco: jcalarco@fas.harvard.edu These plasmids are descirbed...plasmids from Dr. John Calarco's lab. CRISPR...Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco JA. Nat. Methods . 2013 Jun. Nature Methods . PubMed...

...see these vectors, visit Addgene’s website and “search for “pLKO”“. Back to Top B. Designing shRNA Oligos...example, the Whitehead Institute for Biomedical Research hosts an siRNA Selection Program that can be accessed...Starting at 25nt downstream of the start codon (ATG), search for 21nt sequences that match the pattern AA(N ...N 19 ). If no suitable match is found, search for NAR(N 17 )YNN, where N is any nucleotide, R is a purine...

...-Bsa4 as an example of LIC experimental design. Search Addgene's collection of empty LIC cloning vectors...transformation, following a standard protocol . Search Addgene's collection of empty LIC cloning vectors...

...adeno-associated virus (AAV) preps for use in your research.... as an intravenous isomolar contrast agent. In research, iodixanol is used as an isomolar density gradient...

.... From a single copy of DNA (the template), a researcher can create thousands of identical copies using...synthesizes and ships them as a lyophilized powder. The researcher then needs to reconstitute their primers in liquid...

...individual cells with cloning cylinders. Last Update: March 21, 2023 Workflow Timeline This protocol begins ...transgene could be lost through recombination. Some researchers choose to maintain cells in selective medium ...

... is mis-called? Open the trace file and use the search feature in the program to locate the incorrect ...

...antibiotic resistance gene on a plasmids allows researchers to easily isolate bacteria containing that plasmid...

...Welch to discuss her career path, differences in research culture between the US and Australia, and advice...

...Introduction Microscopes are emblematic of biological research and are found in many different types of laboratories...

...while the population expands. At this time, some researchers reduce the concentration of the antibiotic in...

...antibiotic resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid...

...reverse-complement using existing software (a quick internet search will lead you to here and many others). If we ...