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We narrowed to 599 results for: arl

Showing: 261 - 280 of 599 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... close to the hood so you can view your cells regularly (both before entering the hood and when putting...Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You...

  2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7... and base editing, to more loci. Although these early data are exciting, there’s a lot we still don’t ...

  3. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...may be too constraining for some experiments, particularly in vivo ones. In those cases, AAV vector screens...your CRISPR screen to a high degree. This is particularly helpful when investigating tissue-specific diseases...

  4. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...experience, the more I heard that getting into industry early would only help. Like any major life decision, ...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...seen some major advances in light microscopy, particularly related to the neurosciences.  These developments...tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl Deisseroth’s lab developed CLARITY, which is a hydrogel-based...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...The maturation of the chromophore on many FPs (particularly those derived from GFP) requires oxygen. Therefore...interested in everything related to gene expression, particularly at the RNA level. He maintains the greenfluorescentblog.wordpress.com...

  7. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...experiences. STEM in elementary schools - catching them early I’m fortunate to live in a town with a good public...means it was the best kind of networking! Since nearly every talk I’ve given in the past 20 years has ...

  9. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...didn’t attend. For example, we got a good bit of early feedback from those who wanted, but were unable ...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed...

  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Lentiviral vector history and components In the early 1990s, researchers developed viral vector systems... but this research is still in the pre-clinical/early clinical stages. Researchers are also working to...

  15. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...the introduction of this mutation can be any site early in the coding region of the gene of interest. This...Repressing target genes using dCas9-based repressors Early experiments using dCas9 in bacteria demonstrated...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    ... to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions of ...us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal software ...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however...DETECTR. How does DETECTR work? DETECTR works similarly to SHERLOCK. Cas12a is targeted to a specific ...

  19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, ... Assays that require recognition of ions or particularly small molecules that do not generate an immune...

  20. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...easier to find materials and learn about them. As early as 2007, we began grouping similar tools together...12 guides available on our website, which are regularly used by scientists around the world. Collectively...
Showing: 261 - 280 of 599 results