We narrowed to 600 results for: arl

...creates chimeric circles of DNA that are distant linearly but close together in space. The chromatin “picture...enhancer is interacting with. Super enhancers are particularly good models for building chromatin interaction...

... close to the hood so you can view your cells regularly (both before entering the hood and when putting...Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You...

...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7... and base editing, to more loci. Although these early data are exciting, there’s a lot we still don’t ...

...cytometry (“spectral flow”) has emerged as a particularly useful tool for those wishing to run larger ...Mayo Clinic studying host-microbe interactions in early life.    Resources More resources on the Addgene...

...may be too constraining for some experiments, particularly in vivo ones. In those cases, AAV vector screens...your CRISPR screen to a high degree. This is particularly helpful when investigating tissue-specific diseases...

...experience, the more I heard that getting into industry early would only help. Like any major life decision, ...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

...seen some major advances in light microscopy, particularly related to the neurosciences.  These developments...tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl Deisseroth’s lab developed CLARITY, which is a hydrogel-based...

...The maturation of the chromophore on many FPs (particularly those derived from GFP) requires oxygen. Therefore...interested in everything related to gene expression, particularly at the RNA level. He maintains the greenfluorescentblog.wordpress.com...

...didn’t attend. For example, we got a good bit of early feedback from those who wanted, but were unable ...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed...

...experiences. STEM in elementary schools - catching them early I’m fortunate to live in a town with a good public...means it was the best kind of networking! Since nearly every talk I’ve given in the past 20 years has ...

...conjugate, and the complexity of the system. A particularly desirable property for small-molecule labeling...removing excess dye from the sample, which is particularly important for samples where washing is difficult...

...information while using less resources and are particularly useful when trying to screen a limited number...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...

...mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly distant regions close...

...Lentiviral vector history and components In the early 1990s, researchers developed viral vector systems... but this research is still in the pre-clinical/early clinical stages. Researchers are also working to...

...the introduction of this mutation can be any site early in the coding region of the gene of interest. This...Repressing target genes using dCas9-based repressors Early experiments using dCas9 in bacteria demonstrated...

... to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions of ...us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal software ...

... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, ...

...CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however...DETECTR. How does DETECTR work? DETECTR works similarly to SHERLOCK. Cas12a is targeted to a specific ...

...conferences should strive to provide support to early career scientists, students, and those from historically...Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael...

...reprogramming from 0.001% to nearly 0.1% [7]. In terms of hiPSC colonies, nearly 16 hiPSC colonies can now...