We narrowed to 643 results for: ato
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TypeBlog Post...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...
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Cellosaurus: A Cell Line Information Database
TypeBlog Post...line is known to be genetically manipulated, its anatomical origin, if cell line has ever been the target...informative. Spoiler alert: HeLa is the most common contaminator line. For those wanting to access Cellosaurus... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...metadata for each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system...CRISPR database are encouraged to contact BioGRID curators at [email protected] to submit their published... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog Post...through Caitlin’s blood. “I come from a family of educators, mostly elementary school, middle school, and ...school, I actually served as the student course coordinator. With the guidance of one of the faculty members... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...For this series, I’ll interview three science communicators who work in the biotech, education, or nonprofit...job. “I am really more of a multimedia science communicator than a science writer these days. I do some ... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post... more than 100,000 samples to researchers and educators in 60 countries each year. Introducing Arabidopsis...Science and Industry (COSI) and the Franklin Park Conservatory. Do you use Arabidopsis in your research? Are... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that...Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio, a system... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...between the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down... -
Celebrate National Book Month with Addgene
TypeBlog Post...relevant science." - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and ... Tsang, Science Communications and Marketing Coordinator Non-science favorites The Mistborn series by... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...37825 AAV1 pAAV-CAG-GFP 59462 AAV2 pAAV-CAG-tdTomato (codon diversified) 114472 AAV5, AAV8 pAAV-...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120 AAV1 pAAV-ihSyn1... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...serotonin is a well-known but poorly studied neuromodulator, and evidence suggests that it is incredibly...sensor). In preliminary studies with the lab’s collaborators, they were able to use the serotonin sensor ... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog Post... at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity. Ideally, this number should be ...like a PCR product). This method also gives an indicator of DNA or RNA contamination based on the presence... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry, microbiology...the time I was ready to graduate with my BSc in Anatomy, the University of Otago had established the Centre... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation... -
Science Career Options
TypeBlog Post...skills include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor..., including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...bodies do for you? R bodies are robust linear actuators that could be used in a variety of applications...applications, purified R bodies could also perform as actuators in microelectromechanical systems (MEMS) devices...