We narrowed to 376 results for: bre

1. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   ...transduced at all by this serotype. Nichole Beebe in Brett Schofield’s lab demonstrated that pathways projecting...

2. Viral Vectors 101: Viral Applications

   Type

   Blog Post

   ...Zaunders JJ, Gabuzda D, Crowe SM, Mills J, Lewin SR, Brew BJ, Cunningham AL, Churchill MJ. Pathogenicity and...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce...

4. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   ..., as their access to the host genome requires breakdown of the nuclear envelope, which occurs during mitosis...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   ...offers high efficiency of inducing double strand breaks and more opportunity for homologous recombination...

6. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   ... When expressed, the β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo...

7. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   ... you are interested in and this is a great ice breaker. Of course, real relationships go further and grow...

8. 3 Challenges in Plant Synthetic Biology

   Type

   Blog Post

   ...the day cannot be developed through conventional breeding; synthetic biology is required to achieve this...

9. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

   Type

   Blog Post

   ...(A) Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible. ...

10. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    ...schedule might work. If not, perhaps a 3 hour breakfast once a month. All participants must commit to ...

11. dTAG - You're it!

    Type

    Blog Post

    ...not necessary for the proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693....

12. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    ... tags influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific...

13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    ...study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors...

14. RNA Extraction Without A Kit

    Type

    Blog Post

    ...broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late 1970’s, Chirgwin...

15. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    ...obvious explanation, but more often than not I had to break down exactly what I had done in order to identify...

16. What's New in CRISPR - November 2021

    Type

    Blog Post

    ...transcriptase (RT), which induces two double-strand breaks and deletes the intervening sequence. As with other...

17. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    ...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...

18. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    ...transcription instead of introducing a DNA double strand break. Transient introduction of CRISPRi or inducible ...

19. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    ...function of guiding Cas9 to generate a targeted break. These advances in gRNA technology have enhanced...

20. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    ... different stages of development, ensuring the breadth and versatility of this tool. Find CreLite at Addgene...