We narrowed to 369 results for: epor

1. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   ...of posts explains how to critically assess the reported properties of fluorescent proteins, how to do ...

2. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...

3. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ...detection limits of about 5% mutant targets have been reported for mismatch nuclease assays (2, 6, 7). Ability...

4. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   ...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...

5. Hot Plasmids - February 2022

   Type

   Blog Post

   ...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

6. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   ...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...

7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   ...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   ...knockout screening). As the Zhang lab's Nature paper reports, “Here we have shown that the SAM system is robust...

9. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   ...where particular variants or mutations have been reported in people, we can use this kit to knock in those...

10. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    ...research. But be aware that the fluorescent proteins reported in publications are only characterized under a...

11. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    ...buffered against the effects of copy number on reporter gene expression. On the other hand, using a constitutive...

12. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    ...identify highly biotinylated background proteins reported by other research groups that performed BirA*-...

13. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    ...problem, you might even be expected to write up a report to communicate your findings to your colleagues...

14. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    ...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators...

15. Seven Tips for Using LinkedIn as a Scientist

    Type

    Blog Post

    ...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that...

16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    ...indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893. A MoClo toolkit for...

17. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    ...donors such as fundraising materials and progress reports, in addition to articles for the Fred Hutch website...

18. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    ...rapidly screen thousands of proteins for one that can report electrical activity via imaging. They took the ...

19. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    ...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...

20. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    ...of Research on Women’s Health, emphasizes that reporting the sex of cells and tissues—when that information...