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We narrowed to 636 results for: erf

Showing: 261 - 280 of 636 results

  1. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...academia, is someone who visits an institution to perform research there temporarily. The length of stay ...relevant literature for the techniques you will be performing to familiarize yourself. Identify reagents you...

  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig...Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments for discovering novel...

  4. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...lentiviral vectors from the repository for you. We perform test infections with all of our viral preps and.... We provide enough lentivirus for you to both perform an optimization-scale infection and at least one...

  5. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ... editing experiments in this publication were performed using a technique called nucleofection, which ...found on our plasmid pages. We encourage any labs performing genome editing experiments to try nucleofection...

  6. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...blue and emission spectra are red. Neither is a perfect match to the filter set but the excitation filter...reported brightness measurements. Here, EGFP outperforms the improved folding variants, presumably due...

  7. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...efficient selection. This is usually achieved by performing a "kill curve" (basically growing cells in various...Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone Read More: Plasmids 101: Antibiotic...

  8. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...intense student engagement and joy, rather than a perfect lesson plan or outstanding test scores.  As a result...Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro, and the...

  9. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9 nuclease, which...generating a monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top ...

  11. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was cloned into a ...frequency within functional light chain sequences. By performing a BciVI digest of the PCR products prior to cloning...

  12. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...access neurons, and study their connections, in a powerful way. Using brain structure to understand brain...exhibit retrograde transport and can therefore perform retrograde tracing, but often at the cost of toxicity...

  13. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...quite different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell... strand to yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur...

  16. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...asked to draft a personal statement, which is the perfect opportunity to paint a comprehensive picture of...technology, background, and lighting for a smooth performance. Most importantly, make certain that everyone...

  17. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...occurs prior to ligation as incompatible DNA ends interfere with that step. Accordingly, NHEJ has a vast toolbox...from the broken end after cleavage, which may interfere with loading of Ku and normal NHEJ activity. Other...

  19. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration....

  20. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...similar rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing ...advancement in our understanding of how CRISPR will perform in embryos but also shows that many more experiments...
Showing: 261 - 280 of 636 results