We narrowed to 388 results for: nans

1. Summer SciComm Series: A PhD in Science Communication

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   ...little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990). More specifically, they were engaging...

2. Revamp Your Lab Meetings With Creative Virtual Collaboration

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   ...Wuchty S, Jones BF, Uzzi B (2007) The Increasing Dominance of Teams in Production of Knowledge. Science 316...

3. Cultivating Community Science at BosLab

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   ...experiments. For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept...

4. Plasmids 101: NGS Quality Control for Pooled Libraries

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   ...proper use requires careful amplification and maintenance of good representation. Addgene wishes you luck...

5. Plasmids 101: Mammalian Vectors

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   ...is typically more (double) than that used for maintenance of a transfected cell line.  Keep these tips ...

6. Targeted Mutagenesis with EvolvR

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   ...Nature 560.7717 (2018): 248. PubMed PMID: 30069054. Sadanand, Saheli. "EvolvR-ing to targeted mutagenesis."...

7. Why and how to start studying chromatin (with Addgene's help)

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   ...JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive...

8. Plasmids 101: Inducible Promoters

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   ...expression and largely defines the amount of recombinant protein that will be produced. Many common promoters...

9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical...

10. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    ...Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319...

11. AAV Q&A with Tim Miles

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    Blog Post

    ... he kindly took some time to address all your unanswered questions via text! (well, maybe not all of them...

12. Antibodies 101: ChIP

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    ... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

13. Antibodies 101: Buffers, Storage, and Conjugates

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    ... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics: Antibodies...

14. Antibodies 101: Monoclonal Antibodies

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    ...from the hybridoma into a plasmid to create a recombinant antibody.     Figure 2: Changes to the...

15. Clear and Accessible Writing

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    ... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ...

16. Viral Vectors 101: Viral Vector Elements

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    ... it to evade detection by the host. Of note, recombinant adenovirus is missing the endogenous E1 gene,...

17. Viral Vectors 101: Types of viruses

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    ...is not currently known to cause any diseases. Recombinant AAV largely does not integrate, and the addition...

18. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    ...homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge SJ. Nat Methods....

19. GPCRs: How Do They Work and How Do We Study Them?

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    ...detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog ...

20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    ...minimized with high powered research designs. Unanticipated discoveries Finally, there are good reasons ...