...rather late stages (i.e. postdoc level) and can include the pressure to publish in high-impact journals...for insights from my time in technical support including tips, advantages, and disadvantages of this overall...without being too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting ...

...If you choose this strategy, it’s important to include the proper protein expression elements (ribosome...entry clones available for many popular genes, including Hs.KRAS4a. Use our website to search for your ...Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression Systems...

...the origin and propagation of the line, and can include assays for authentication and contamination) and...International Cell Line Authentication Committee includes a database listing all cross contaminated or misidentified...working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune, and Infors & Applikon...

...expression strains to accommodate high protein levels including:  ompT: Strains harboring this mutation are deficient...Rhamnose-tunable T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have ...insoluble globs of misfolded protein are known as inclusion bodies, and can be recovered and purified using...

...have created synthetic promoters, which typically include some combination of other promoter elements, and...promoters for eukaryotes and prokaryotes We have included two reference tables below listing some of the... simian vacuolating virus 40 Constitutive  May include an enhancer. PGK1 (human or mouse) General expression...

...you realize whether or not you have a natural inclination for this skill that you could strengthen. The...everyone who visits your poster to agree with your conclusions. That conversation you’re having--that conflict...of the conflict. It can be easy to leap to the conclusion that a conflict is personal (”Person A and Person...

...qualitative and quantitative measurement of processes including RNA movement by tagging RNA sequences with an ...Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach...aptamer fluorophores, more are in development, including Carrot and Radish from the lab that developed ...

...chloramphenicol-resistant strains, we have also included versions with gentamicin-resistance (amplified...broad group of Gram-negative Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter...where conjugation may be difficult, we have also included versions of pTOX that encode one of two visible...

...choose a looser gate here. I am not worried about including irrelevant events in my analysis, as these will...Lastly, you learned which gates should always be included in your analysis, regardless of your experiment...Sharpe at Harvard Medical School. His projects include investigating negative regulators of T cells and...

...integrins are key to a diverse array of processes, including cell adhesion, migration, and signaling, as well...available through Addgene’s repository. The collection includes 26 unique antibodies that bind discrete subunits...domains. All bind specific integrin alpha subunits, including αL, αM and α5. IPI’s anti-integrin rAbs are unconjugated...

...have been used to purify a variety of peptides including antibiotic efflux pumps and ankyrin seven-repeat...constructs. Some applications of plasmids from this kit include the production of a suicide switch in cyanobacteria...PUF protein fusions reported in the literature include RNA detection, RNA cleavage, and translational ...

...N-terminus, but you don’t have the tag already included in the vector you want to use. If you inserted...primers to amplify the two fragments while also including regions of homology to the vector or neighboring...sequences between fragments. Some of these products include: GeneArt® Seamless Cloning and Assembly (Thermo...

...resources needed to accomplish your science, and can include: equipment, reagents, animals, glassware, and even...the institution/funding source. Major equipment includes large centrifuges, sequencers and analyzers, and... as many of these tools as possible, this even includes clocks, timers, and phones.  As for reagents and...

...works. Advances in Cre-lox recombination have included inducible Cre, such as the tamoxifen-responsive...immediately following the promoter. The STOP cassette includes a mutant YFP that does not fluoresce, but can ...More variants have been created by other labs, including R26R-Confetti described in Hugo J. Snippert, et...

...phenotype in order to conclude that the phenotype is due to an on-target effect. Conclusions Selection of gRNAs...modification of interest, it would be unwise to make conclusions on the basis of the activity of a single gRNA...

...expression, but causality and mechanisms remain unclear. Today, targeted epigenetic modification at specific...which uses light to regulate transcription, also includes light-regulated histone methyltransferases and...has deposited mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300...

... cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive chimeric GPCRS (OptoXRs) (Tichy...experiments!  First, closely read the publication, including the supplemental data. You’re most likely to find...hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide a powerful way to...

.... Other practices that fall into this category include performing experiments with too few replicates ...institute better experimental design practices including talking to statisticians when necessary and encouraging...Hines Minisymposium on Reproducibility Booklet - Including descriptions of many organizations promoting reproducibility...

...mentioned above. The initial IMP cohort of 78 mentees included: 47 women (60.2%) 20 graduate students (25.6%...directly attributable to participation in the program included improvements in writing fellowships, giving oral...impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary of Research, ...

...Wild-type A549 cells do not express CAs9 and are included as a control (WT). Vinculin is a loading control... assay before, consider following their tips, including: Before digesting the DNA duplexes with Surveyor...PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene...