We narrowed to 457 results for: nsf

...identical targeting vectors delivered through transfection of naked DNA. Other investigators who had been...then packaged into AAV virions via transient transfection of 293T cells with helpers, and used to infect...

...hour or so, the sample is immediately ready to transform into competent cells. The master mix of enzymes...Gibson assembly master mix for 1 hour, and then transform into competent cells. The success rate of this...

...Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR constitutive expression of...+), λ– EPI300   Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC...

.../cJ and CBA/J mice. AAV1-X1 was developed by transferring the 7-mer peptide region from variable region... viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Nature...selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology, 34(2...

... (AAV) Serotype 9 Provides Global Cardiac Gene Transfer Superior to AAV1, AAV6, AAV7, and AAV8 in the ...to 2,3-linked sialic acid is required for gene transfer. The Journal of Biological Chemistry, 276(23),...

...careers?    Will you be encouraged to develop transferable skills by attending career events or being involved...resources on the Addgene blog Check out our transferable skills guide Learn about careers in science ...

...rabbits (Sandoval-Villegas et al., 2021).  Gene transfer Transposons are an alternative to viral vector...(mutagenesis screens, transgenic animals, gene transfer). The discovery of RNA guided transposons in bacteria...

... modified cells are transferred into an animal and the behavior of the transferred cells is followed. ...

...accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to have compatible...and emission profiles to enable effective energy transfer, which is key to observing interactions and conformational...

...molecular weight by SDS-PAGE, and are subsequently transferred to a membrane. A primary antibody recognizing...showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common ...

.... But that doesn’t mean you have to clone and transfect multiple promoter-gRNA constructs in order to ...transient expression and Agrobacterium-mediated transformation are available. For more information on this...

...activation domain (SAD), which recruits histone acetyltransferase to open chromatin, improved editing efficiencies...HMCES, either as a fusion construct or by co-transfection. HMCES forms a chemical crosslink with DNA abasic...

...plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for these methods...Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell,...

...facilities management to website development, tech transfer, lab work, and more. It's this diverse group of...

...science support to our efficient and easy tech transfer process to our website and educational resources...

...you could directly start your digest, PCR, or transformation?  Great news. A subset of our collection has...

...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...

...Sikora’s lab Matthew Sikora’s lab went all out to transform their lab into a walking tour of Buddy the Elf...

...Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA Chapter 4 - Developing...

...developments. These abrupt changes threaten to slow transformative biological discoveries and life-saving medical...