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We narrowed to 457 results for: nsf

Showing: 261 - 280 of 457 results

  1. Five Popular Model Organisms

    Type
    Blog Post
    ...Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences...

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...aptamers are used to track cellular processes by transforming cells with plasmids encoding aptamer-tagged ...expression vectors to create a library. Bacteria were transformed, and then sorted by fluorescence-activated cell...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR constitutive expression of...+), λ– EPI300   Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    .../cJ and CBA/J mice. AAV1-X1 was developed by transferring the 7-mer peptide region from variable region... viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Nature...selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology, 34(2...

  6. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... (AAV) Serotype 9 Provides Global Cardiac Gene Transfer Superior to AAV1, AAV6, AAV7, and AAV8 in the ...to 2,3-linked sialic acid is required for gene transfer. The Journal of Biological Chemistry, 276(23),...

  7. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...careers?    Will you be encouraged to develop transferable skills by attending career events or being involved...resources on the Addgene blog Check out our transferable skills guide Learn about careers in science ...

  8. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...rabbits (Sandoval-Villegas et al., 2021).  Gene transfer Transposons are an alternative to viral vector...(mutagenesis screens, transgenic animals, gene transfer). The discovery of RNA guided transposons in bacteria...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...molecular weight by SDS-PAGE, and are subsequently transferred to a membrane. A primary antibody recognizing...showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common ...

  12. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...activation domain (SAD), which recruits histone acetyltransferase to open chromatin, improved editing efficiencies...HMCES, either as a fusion construct or by co-transfection. HMCES forms a chemical crosslink with DNA abasic...

  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    .... But that doesn’t mean you have to clone and transfect multiple promoter-gRNA constructs in order to ...transient expression and Agrobacterium-mediated transformation are available. For more information on this...

  15. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...facilities management to website development, tech transfer, lab work, and more. It's this diverse group of...

  16. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...science support to our efficient and easy tech transfer process to our website and educational resources...

  17. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...

  20. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...Sikora’s lab Matthew Sikora’s lab went all out to transform their lab into a walking tour of Buddy the Elf...
Showing: 261 - 280 of 457 results