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We narrowed to 416 results for: plasmids 101

Showing: 261 - 280 of 416 results

  1. Technical Design of a Western Blot

    Type
    Blog Post
    ...Antibodies 101: The Basics of Western BlottingAntibodies 101: Selecting the Right AntibodyAntibodies 101: Secondary...recombinant antibodies (rAbs), which are produced from plasmids and have very little batch-to-batch variation.... of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal, recombinant...Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.00...

  4. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ... Addgene.org Browse CRISPR plasmids for base editing Read our CRISPR 101 eBook Brush up with our CRISPR...Additional resources on the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors Progress Towards...of CRISPR. Cell, 164(1), 18–28. https://doi.org/10.1016/j.cell.2015.12.041  Musunuru, K., Grandinette, ...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies... readily accessible recombinant antibodies and plasmids. To encourage collaboration and education in protein.... 2024.01.26.577394). bioRxiv. https://doi.org/10.1101/2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé...

  6. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...transforming both fusion plasmids, or mating two single haploid yeast fusion plasmid transformants into a ...reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example...protein interactions. Proc Natl Acad Sci U S A. 2004; 101(33):12242–12247. Pubmed PMID: 15299147. Pubmed Central...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our...

  7. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...summer or just a bright new plasmid. One area with lots of bright new plasmids is our Fluorescent Biosensors...Proteins 101: Fluorescent Biosensors Resources on Addgene.org Fluorescent protein plasmids and resources...SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3 plasmids here! Find viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.... 2022, under CC-BY-NC license.   Find JEDI-2P plasmids here! Find viral preps here: pAAV-EF1a-DIO-JEDI...et al. 2022, under CC-BY license.   Find pYtags plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal... suitable for AAV packaging.    Find iGECInano plasmids here!   Matlashov ME, et al. Design and Initial...CC-BY-NC-ND license.   Find KRaION and Kbp sensor plasmids here!   Torres Cabán CC, et al. Tuning the Sensitivity...

  8. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis...amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence of an insert...

  10. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ... Genome Engineering Guide Plant Plasmids and Resources CRISPR Plasmids and Resources ...for ωRNA (reRNA) expression (see Figure 1). Both plasmids contain a hygromycin resistance gene as the plant-selectable... 2–3 positive colonies, grow them, and extract plasmids. Confirm the correct guide insertion by Sanger...primer. Step 7: Transformation Your confirmed plasmid is now ready for Agrobacterium-mediated, biolistic...Additional Resources on the Addgene Blog CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 RNA...

  11. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria." Plasmid 51.3 (2004): 246-255. PubMed...Additional resources on the Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn ...exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations...a mutated allele introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile... These two steps are (Figure 1): The initial plasmid integration step (the so-called “single crossover... an allelic exchange experiment? Find the pTOX plasmids here! Continued development of new allelic exchange...recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our molecular biology...

  12. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...your plasmid; separate plasmid DNA from unwanted cellular “debris”; and then clean-up the plasmid DNA ...debris from the plasmid, and then clean-up the plasmid so that you are left with pure plasmid DNA. Image created...Addgene blog Plasmid Preps: Different Purity, Different Quantities, Different Uses Plasmids 101: Optimizing... help you choose the plasmid prep kit that is right for you. What type of plasmid prep do you need? As...As we covered in a previous post on plasmid preps, not all plasmid preps are the same. Your downstream...arise during the plasmid purification process. One is the possibility of damaging the plasmid DNA itself, ...carrying over contaminants from the plasmid prep process into the final plasmid eluate. Possible contaminants...

  13. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...chemical biology 9.10 (2014): 2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid ...

  14. Caltech Systemic Capsids

    Type
    Collection
    ...use (Link opens in a new window) . Viral Vectors 101: Systemic Capsids PHP.eB These viral vector preparations...preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and ...preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ...preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847) . Browse Available PHP.V1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136) . Browse Available MaCPNS1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137) . Browse Available MaCPNS2 AAV...were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004) . Browse Available CAP-B10 AAV...

  16. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ... CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...of sterile truncated pipet tips (USA Scientific #1011-8410) is also placed in the hood. Finally, a sterile...

  17. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial...

  18. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Validation Resources on addgene.... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...

  19. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting...the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in ..., the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected...provided (in the form of an oligonucleotide or plasmid), precise changes can be made in the genome via...mice carrying the intended mutation. With a donor plasmid, the outcome is less predictable. In our best case.../srep32565 Resources on the Addgene Blog CRISPR 101: Mammalian Expression Systems and Delivery Methods...Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci.2018.10.030 Qin W, Kutny PM, Maser RS, Dion...

  20. Design Tips for Prime Editing

    Type
    Blog Post
    ...the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by function...function Browse our entire plasmid collection ... Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph, P. ...Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P....
Showing: 261 - 280 of 416 results