We narrowed to 352 results for: plat

1. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

2. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ... 26436575. PubMed Central PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for...

3. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   ... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown...

5. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...cleavage”. Jacob computationally added the non-template strand to the Jinek structure so that the resulting...

6. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...

7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   Blog Post

   ...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...

8. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   Blog Post

   ...chromosome and its own gene is used as a repair template, thereby converting the heterozygote into a homozygote...

9. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   ...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...

10. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    ... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

11. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ...., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression...

12. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post

    ...Cre-expressing tissues. This inversion creates a template that’s suitable for PCR amplification using pre-designed...

13. MXS Chaining

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    Blog Post

    ...Neveu PA. MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian...

14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...

15. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

16. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...

17. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    .... (2020). Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally...

18. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    ...Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology, 79(1), 163–183....

19. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ... strain for screening), or arrayed on a 96 well plate where each well contains a defined prey fusion.  ...

20. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...confirmed. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Comparing Cpf1...