We narrowed to 358 results for: plat

1. Plasmids 101: Terminators and PolyA signals

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   Blog Post

   ...100% efficient at halting transcription of the template and initiating the desired cleavage event, although...

2. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown...

4. 3D Printing Meets CRISPR Cas9

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   ...cleavage”. Jacob computationally added the non-template strand to the Jinek structure so that the resulting...

5. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

6. Plasmids 101: Protein Expression

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   ...expression consists of transfecting cells with your DNA template of choice and allowing these cells to transcribe...

7. Tips to Make the Most of a Scientific Conference

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   Blog Post

   ...’s best to turn to an established social media platform. During the conference If the conference has a...

8. Truncated gRNAs for Regulating Gene Expression

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   ... 26436575. PubMed Central PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for...

9. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...

10. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post

    ...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...

11. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...chromosome and its own gene is used as a repair template, thereby converting the heterozygote into a homozygote...

12. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...

13. Viral Vectors 101: The Retroviral Lifecycle

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    ...Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology, 79(1), 163–183....

14. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...Cre-expressing tissues. This inversion creates a template that’s suitable for PCR amplification using pre-designed...

15. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

16. Isolating Genomic Regions of Interest with the CRISPR System

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    ...., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression...

17. CRISPR 101: RNA Editing with Cas13

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    ...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...

19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...

20. Negative Can Be Positive: Open AAV Data with Addgene

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    .... (2020). Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally...