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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 538 results for: sequence

Showing: 261 - 280 of 538 results

  1. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...PCR is most commonly used in molecular cloning, sequencing, and genotyping. It’s extremely useful but is...such as in colony PCR, or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase...replicates. ddPCR can be used to quantify DNA sequences that are rare, for example, rare alleles or mutations...

  2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...CRISPRi cassette flanked by left and right Tn7 end sequences. Donor bacteria with a chromosomal copy of the...recipient bacteria due to site selection by TnsD, a sequence-specific DNA binding protein. Selection removes...DNA (purple) flanked by left and right Tn7 end sequences (green) integrates downstream of the glmS gene...

  3. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ... repeating elements anymore. The kit contains sequences for several different fluorescent proteins. Visualizing... of work on wheat rusts and we are collecting sequences that will provide a similar resource for those...they aren’t sure how to convert their own coding sequence to the MoClo system. It’s quite straightforward...

  4. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...pH can denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds...backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination. In depurination...

  6. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...addition to peer review than having your plasmids sequenced and openly registered for other scientists to ...we won’t distribute materials with unresolved sequencing issues or if you’ve asked to cancel the deposit...

  9. Antibody Production

    Type
    Collection
    ... purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence to confirm ...protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody preps contain low...

  11. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...the cap plasmid library by inserting short DNA sequences that contain all possible combinations into a ...plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid pool ... animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues...

  12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...ten base pairs in length. An emerging family of sequencing techniques function as a kind of “GPS for the...over time, we periodically use next-generation sequencing to measure the relative proportion of guide RNAs...insulators, and more. I then identified valid gRNA sequences without off-target effects for the 2+ million ...

  13. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular gRNA. In the enChIP ...For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding ...

  16. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...Rosella. When fused with a mitochondria-targeting sequence, Rosella can be used to track the targeted autophagy..., but the addition of a mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It...

  18. TALEN Engineering

    Type
    Collection
    ...produced by all three methods are identical in sequence. TAL effector repeat arrays produced on this framework... ZiFiT Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional...

  19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs into one... systems: St1Cas9 requires a very specific PAM sequence that limits the number of targetable loci, and...enrichment on streptavidin and next-generation sequencing). Using this sensitive method, they found that...

  20. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...edits (St. Martin et al., 2018). To circumvent sequencing and the need for a DSB in their previous reporter...need for single cell isolation and downstream sequencing (Standage-Beier et al. 2019). TREE is a real-...codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized that if they created...
Showing: 261 - 280 of 538 results