...Pennsylvania independently searched for this unique sequence of DNA and both identified lymphocyte antigen ...as a receptor for PHP.B by looking for a unique sequence of DNA that was present in mice whose CNS was ...alleles that was driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to...

...the cap plasmid library by inserting short DNA sequences that contain all possible combinations into a ...plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid pool ... animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues...

...ten base pairs in length. An emerging family of sequencing techniques function as a kind of “GPS for the...over time, we periodically use next-generation sequencing to measure the relative proportion of guide RNAs...insulators, and more. I then identified valid gRNA sequences without off-target effects for the 2+ million ...

...antibodies will come with access to the antibody sequences, in line with the rest of Addgene’s recombinant...

...Rosella. When fused with a mitochondria-targeting sequence, Rosella can be used to track the targeted autophagy..., but the addition of a mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It...

... predefined DNA sequence that uses trprimers and enzymes that will amplify a sequence only if present....

...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

...please contact us . Full sequencing of the Viral Genome Next-generation sequencing is performed on viral ...genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient...genomes isolated from the final AAV preparation. Sequencing results are analyzed to confirm the identity ...

...Does my transcription factor bind to a particular sequence? Do these two proteins interact? What happens ...localization: Contains a 2x array of the binding sequence for RNA-binding protein, MS2. Can be used in combination...localization: Contains a 2x array of the binding sequence for RNA-binding protein MS2 fused to dsRED. Can...Tac (lactose/IPTG inducible) MBP, 6x His, signal sequence for localization to the periplasm David Waugh ...N-terminal 6x His tag and an MBP tag. Contains a signal sequence to localize the protein of interest to the periplasm...of N and C-terminal epitope tags, localization sequences, and cleavage sites that can be used to express...Assembly Accessory Reagents Kit Various Plasmids Sequence chosen by researcher Beta-galactosidase Keith ...

...of target sequence in the 3' UTR David Bartel 12179 pIS1 Renilla Insertion of target sequence in the 3'... Bartel 12177 pIS2 Renilla Insertion of target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla... PCD FL0X Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly... a T7 promoter for normalization. FMDV StopGo sequences flank the MCS for improved normalization. John... mpCHECK2 Firefly/Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly luciferase...psi-CHECK3 Firefly/Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly luciferase...

...However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs into one... systems: St1Cas9 requires a very specific PAM sequence that limits the number of targetable loci, and...enrichment on streptavidin and next-generation sequencing). Using this sensitive method, they found that...

...edits (St. Martin et al., 2018). To circumvent sequencing and the need for a DSB in their previous reporter...need for single cell isolation and downstream sequencing (Standage-Beier et al. 2019). TREE is a real-...codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized that if they created...

...targeting system could be used to routinely deliver sequences of interest to cells with efficiencies of 1-40%...CRISPR is remarkably effective for introducing non-sequence specific frameshift mutations through aberrant...template. It is this application - the creation of sequence-specific knockins - for which the AAV approach...currently focuses on determining the mechanisms and consequences of cohesin gene inactivation in human cancer...

... however, Cas9 allows you to easily change the sequence targeted by the activator by simply providing ... consist of plasmids that over-express coding sequences from a given cell type or organism, have been ...several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.     References ...

... purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence to confirm ...protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody preps contain low...

...pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published a case study...

...host machinery to make additional copies. ori sequences are generally high in As and Ts. Why, you ask?...that affect plasmid copy number  Although the sequence and regulation of the ori dramatically affect ...

...often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, fluorescence-based...method is often used preceding next-generation sequencing or microarray studies and is not used as much...

.... Different antibodies bind different protein sequences called epitopes and scientists can use these to...different targets, the scientists fused them to sequences called complementarity determining regions (CDRs...

...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...