We narrowed to 526 results for: sequence

...N-terminal consensus sequence (MG-) and prenylation requires a C-terminal consensus sequence (-CaaX). Both modifications...modifications are disrupted when GFP is fused to these sequences. When N- and C-terminal fusions are not tolerated...alternative is to insert the GFP into the coding sequence of the protein of interest. One of the reasons... reaction requires molecular oxygen and as a consequence GFP remains non-fluorescent under anaerobic conditions...

...segments, each with two fluorescent protein coding sequences in opposite orientations. Cre-mediated excision...the system, the fluorescent protein whose coding sequence is closest to the promoter will be expressed. ...used are fluorescently distinct, their protein sequences are highly homologous, preventing design of antibodies...three with low levels of both fluorescence and sequence overlap (coral mOrange2, jellyfish EGFP, and sea...

...MG1655    Wild type K-12 strain; first published sequence of an E. coli K-12 strain. F- λ- ilvG- rfb-50 ...and retroviral vectors or cloning or repeated sequences with the potential to recombine.  F' proA+B+ lacIq...and retroviral vectors or cloning or repeated sequences with the potential to recombine.  F- endA1 glnV44...and retroviral vectors or cloning or repeated sequences with the potential to recombine. This strain is...E.coli strains Gene(s) Description Functional Consequence dam DNA adenine methylase mutation (GATC) Preparing...

...strange breaks at target sites 5' of the 5'NGG3' PAM sequence as directed by a gRNA. 2A-Puro In this plasmid...into which you can clone your 20 nucleotide guide sequence of choice (for generation of a gRNA that will ...

...phosphorylatable residues and the bNLS and NES sequences, which have previously been characterized. See...more details on the design of the bNLS and NES sequences. Disadvantages of KTRs KTRs also have some disadvantages...

...using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive genomic locus...

...These plasmids are created by your colleagues, sequence-verified, and widely cited in publications. All...

...assessment, sterility testing, and viral genome sequencing (VGS). In-stock AAV preps are distributed as ...

...strategies: Strategy #1 (ProA): Create promoters with sequences upstream of the start codon of retinal cell-type...Create promoters using phylogenetically conserved sequences that are upstream of the transcription initiation... factor binding sites interspaced with random sequences.   Strategy #4 (ProD): Create promoters with the...

...in place of SpCas9. gRNA A gRNA targeting a DNA sequence of interest is expressed in 293T cells and loaded... HDR allows for the precise insertion of a DNA sequence ranging from a single nucleotide change to large...utilize HDR, a donor template containing the desired sequence flank by homology arms must be delivered to cells...

...approaches: single-cell RNA-sequencing to identify neuronal class and deep sequencing of neurons from each class...of a GAL-4 driver that can bind to activation sequences upstream of genes to stimulate expression (Wang...

...In the O'Connor lab, we recognized that genome sequencing projects were generating huge amounts of data...available. Then, staff or students clone similar sequences from a less-well studied organism into overexpression...experiments We used TOPO cloning to insert the coding sequences for ten different MET/Met genes (Figure 1) from...

... RNA sensors that can detect virtually any RNA sequence (Green et al, 2014). The second was an in vitro...laboratory. The detection threshold for these RNA sequences (3 nM), however, was quite high and above what...and prototyping pipeline that can take pathogen sequence information from public databases to the production...

...that recognize, cut, and alter specific target sequences in DNA resulting in a variety of outcomes including... requirement. For instance, for Casɸ-2 the PAM sequence is 5’-TBN-3’ (where B is G, T, or C). The authors...scientists seeking a wider range of targetable genomic sequences could benefit from CRISPR-Casɸ. Pausch et al....

...expression that do not involve any changes to DNA sequence (Epigenetics literally means ‘on top of the genome...with reduced life-span, but no changes in the DNA sequence could be found. Angelman syndrome and Prader–Willi...methyltransferase identification, and bisulfite sequencing. However none of these techniques can be used...

...Weinstein RNA aptamers, which are nucleic acid sequences that fluoresce upon binding a small molecule, ...From considering protein size and shape, linker sequences, and fusion location, there are many choices to...

...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...

...considered "scarless", as the transposon homology sequences are integrated along with the donor DNA. The two...

...gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and clone in the homology arms...2A-PuroR cassette, flanked by microhomologous sequences and gRNA target sites. The first deposited PITCh...

...editing experiment. I was in my office and opened a sequence file. Then I saw it, the chromatogram showing ... signs of a mutation introduced by CRISPR. The sequence started clean and then, about 100 bps into the...the run, it got “dirty”, with multiple sequence traces overlapping each other! When the count was done,... a reporter gene, or replacement of the murine sequence with a human gene. Advantages of using CRISPR ...