We narrowed to 64 results for: sequence

...meningitidis Cas9, or Cas12a from your input sequence or list of sequences. Developed by the George Church Lab ...new window) Identifies gRNA target sequences from an input sequence and checks for off-target binding....window) Identifies candidate gRNA target sequences in an input sequence, which can be an accession number, ..., genomic location, pasted nucleotide sequence, or a sequence text file you upload. Currently supports...program that identifies gRNA target sequences from an input sequence, checks for off-target binding and...window) Identification of gRNA target sequences from an input sequence and checks for off-target binding....

...perfect world, your gRNA sequence would be an exact complement to your target sequence with no homologous sites... the desired target sequence, or on-target activity. Two gRNA targeting sequences with 100% homology to... selected target sequence. Additionally, any mismatches between a given gRNA sequence and your genomic...customization Usually paired with a sequencing method Can’t provide specific sequence information alone Digital ...rare edits Many options like RNA-sequencing, Perturb-seq, deep sequencing, etc. qPCR Knockouts Activation...and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate your genetic edit...within a very narrow window proximal to the PAM sequence Base Edit Prime Edit Generate targeted insertions...

...target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream of...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...they have recognized and cleaved the target RNA sequence, they adopt an enzymatically active state and ... of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed cell death...

... be any ~20 nucleotide sequence, provided it meets two conditions: The sequence is unique compared to ...(PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends on which... target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting sequence) will begin to anneal...matters. Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...determined by both the gRNA sequence and the Cas9 enzyme. Ideally, a gRNA targeting sequence will have perfect ...require PAM sequences in very close proximity to the region to be edited, making the PAM sequence (NGG) limiting...target sequence in your donor DNA with a silent mutation that does not change the amino acid sequence. HDR...

...additional 5' clamping sequence such as GTTTCTTC). Note that all prefix/suffix sequences are given for the ...Assembly standards define standard prefix and suffix sequences that make it easy to combine components for synthetic...sites which preserve a standard prefix and suffix sequence at either end of the assembly, even as more parts...registries, including the specific prefix and suffix sequences for each (these can be built into PCR primers ...prohibited sites and must be omitted from the coding sequence of the part and backbone. For some standards, ...Thr-Gly; Use N-parts prefix to preserve N-term sequence. Prohibited Restriction Sites: EcoRI, XbaI, NgoMIV...

...-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation...express dCas9-VPR for activation. Usage Guide sequences can be designed using Benchling or using similar...Golden Gate cloning to insert the desired guide RNA sequence by digestion of the backbone with BbsI followed...phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can be expressed from the same... Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence Derivative Sources Original pX458 backbone ( Addgene...

...Palmitoylation sequence from GAP43 EGFP Connie Cepko 61099 Lck-GFP Membranes Palmitoylation sequence from Lck...4xmts-mNeonGreen Mitochondria 4x Mitochondrial targeting sequence (COX8) mNeonGreen Dorus Gadella 98819 4xmt-mTurquoise2...4xmt-mTurquoise2 Mitochondria 4x Mitochondrial targeting sequence (COX8) mTurquoise2 Dorus Gadella 98818 4xmts-mScarlet-I...4xmts-mScarlet-I Mitochondria 4x Mitochondrial targeting sequence (COX8) mScarlet-I Dorus Gadella 19031 pABCb10-...pLV-mitoGFP Mitochondria Mitochondrial targeting sequence (COX8) GFP Pantelis Tsoulfas 80000 pMito-miRFP703...pMito-miRFP703 Mitochondria Mitochondrial targeting sequence (COX8) miRFP703 Vladislav Verkhusha 36208 pmTurquoise2...pmTurquoise2-Mito Mitochondria Mitochondrial targeting sequence (COX8) mTurquoise2 Dorus Gadella 26673 pEGFP-C1...

... DNA sequence complementary to the crRNA. In Type II CRISPR systems, a potential target sequence is only...Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in the E. coli genome...loci to generate CRISPR RNAs (crRNAs). Through sequence homology, these crRNAs guide a Cas nuclease to...material, which must also contain a species-specific sequence known as a protospacer adjacent motif (PAM). The...CRISPR array contains many unique protospacer sequences that have homology to foreign DNA. Protospacers...Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed to make the...genomes, are highly diverse, with varying PAM sequences and number/types of Cas proteins. Makarova et ...

...target site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found...target site sequence (30bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 ...target site sequence (21-22bp) and need to be followed on the 3' end by NGRRT or NGRRN PAM sequences. We have... - Contains BbsI sites for insertion of guide sequences Addgene plasmids #61425 - #61427 are intended .... Contains BsmBI sites for insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return...phosphorylated oligos designed based on the target site sequence (20bp). CRISPR-Cas9 Cre expression vectors for...phosphorylated oligos designed based on the target site sequence (20bp). #60230 - AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre...

...details including the protein and plasmid sequence, the antigen sequence, and cross-species reactivity. Reactivity... trusted partner labs or is predicted based on sequence homology with the antigen. Addgene supplies a ...

...DNA prep, the plasmid sequence has been validated by next generation sequencing. The DNA concentration...Quality Control Plasmid verified by next generation sequencing Plasmids Available as Cloning Grade DNA ID Plasmid...

...Use the target site sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-...recombinases and tyrosine recombinases, based on sequence similarity and the amino acid residue used in ...DNA strand (in cis) in the same direction, the sequence between the sites is excised as a circular piece...recombinase systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT...target sites. Codon optimization: Modifying the DNA sequence of the recombinase to align with your organism's...

...transcription initiation when targeted to DNA sequences within core promoter regions. pTAL5-BB contains... place of pTAL1, 2, 3, or 4. For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into ...visualize subcellular positioning of target DNA sequences in living cells (TALE-mediated Genome Visualization...white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient TALEN expression in mammalian...vectors for use in labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for...

...please contact us . Full sequencing of the Viral Genome Next-generation sequencing is performed on viral ...genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient...genomes isolated from the final AAV preparation. Sequencing results are analyzed to confirm the identity ...

... purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence to confirm ...protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody preps contain low...

...produced by all three methods are identical in sequence. TAL effector repeat arrays produced on this framework... ZiFiT Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional...

...Mismatches between the guide RNA and the target sequence lower the repression level. By controlling the...motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP concentration...

...other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists have identified a large...incentives are high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers...Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating mammalian...

... for plasmids that contain a zebrafish gene or sequence . The table below highlights plasmids that contain...Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish Model...zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish...

...of target sequence in the 3' UTR David Bartel 12179 pIS1 Renilla Insertion of target sequence in the 3'... Bartel 12177 pIS2 Renilla Insertion of target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla... PCD FL0X Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly...psi-CHECK3 Firefly, Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly luciferase...