We narrowed to 362 results for: tac

1. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   ...all disposable items and surfaces that come into contact with the rAAV and apply common cell culture practices...

2. PhD Applications After COVID

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   Blog Post

   ...other. Before COVID-19, financial and logistical obstacles could make touring potential programs and universities...

3. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   Blog Post

   ...mimicry and camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to ...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...cultures and later maxiprep only the cultures with intact ITRs. I use a spreadsheet similar to this one to...

5. Anatomy of a Plasmid Page at Addgene

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   Blog Post

   ...about our plasmid pages, please don't hesitate to contact us at [email protected] Resources on the Addgene...

6. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   ...labels (green circles in figure 2 below) can be attached to each antibody. But being big is a double-edged...

7. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ...schools have dedicated science teachers. I tried contacting our school district’s science curriculum coordinator...

8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...randomly, or at fixed genomic target sites.  This obstacle was recently surpassed with the discovery of CRISPR-transposons...

9. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ...rational mutagenesis on the ADAR2DD residues that contact the RNA substrate resulted in 15% C-to-U editing...

10. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...performance) will be assigned citable DOIs through DataCite before being made publicly available through the...

11. Getting to Know Expansion Microscopy

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    Blog Post

    ...sample (i.e. cultured cells or tissues).  Step 2: attach anchors to your sample that will link biomolecules...

12. DNA Purification Without a Kit

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    Blog Post

    ...capture up to 150 ug of DNA, and filters can be stacked together in series to increase binding capacity...

13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ...coming up with no results at all, let us know! Contact our scientific technical support at [email protected]...

14. Tips for arabidopsis transformation

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    Blog Post

    ...transfer DNA (tDNA) into the genomes of the plants it attacks Growing the transformed agrobacterium culture Dipping...

15. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...

16. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...carefully inspected to verify that the samples arrived intact. Some deposits are sent from labs nearby in Boston...

17. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...merit of depositing plasmids to Addgene has been spectacular. We've got more than one hundred requests, and...

18. Your Lentiviral Plasmid FAQs Answered

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    Blog Post

    ...retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing...

19. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ...purified viral DNA as the template instead of the intact AAV particle. Finally, the precision of the assay...

20. Plasmids 101: Gateway Cloning

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    Blog Post

    ...reactions are facilitated by the recombination of attachment sites from the phage (attP) and the bacteria ...